t-PAI-C marker detection kit and preparation method thereof

A technology for detection kits and markers, which is applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of complex operation, low value repeatability, and low sensitivity, and achieve high detection sensitivity, wide linear range, and specificity. good performance

Pending Publication Date: 2020-11-24
深圳市巨东生物医学工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the steps of radioimmunoassay are cumbersome, the reagents are expensive, and supporting instruments are required and radioactive contamination exists.
Enzyme-linked immunosorbent assay has long detection time, complicated operation, poor repeatability, and is not suitable for emergency and clinical patients in time for diagnosis.
Latex-enhanced immunoturbidimetric method is simple and fast to operate, but has low sensitivity and poor reproducibility at low values

Method used

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  • t-PAI-C marker detection kit and preparation method thereof
  • t-PAI-C marker detection kit and preparation method thereof
  • t-PAI-C marker detection kit and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0011] A method for preparing a t-PAI-C marker detection kit is as follows:

[0012] 1.1. Preparation of calibrator 1:

[0013] The tissue-type plasminogen activator-inhibitor 1 complex was prepared into a calibrator with 1%-5% bovine serum albumin solution, freeze-dried, and assigned, and the prepared concentration was 5 ng / mL.

[0014] 1.2. Preparation of calibrator 2:

[0015] Prepare the tissue-type plasminogen activator-inhibitor 1 complex with 1% to 5% bovine serum albumin solution to make a calibrator and lyophilize it for assignment. The prepared concentration is 50ng / mL

[0016] 1.3. Preparation of Reagent 1

[0017] Take 1 mg tissue-type plasminogen activator-inhibitor 1 complex antibody, and ultrafilter it with PB buffer (pH7.0-8.5) at 4°C or room temperature; the tissue-type plasminogen activator after ultrafiltration - Add 0.05 mg of biotin to the inhibitor 1 complex antibody, and then add PB buffer (pH 7.0-8.5) to make the final antibody concentration 0.05-1 μ...

Embodiment 2

[0027] A method for preparing a t-PAI-C marker detection kit is as follows:

[0028] 2.1. Preparation of calibrator 1:

[0029] The tissue-type plasminogen activator-inhibitor 1 complex was prepared with 1%-5% horse serum albumin solution as a calibrator and freeze-dried for assignment. The concentrations were respectively 5 ng / mL.

[0030] 2.2. Preparation of calibrator 2:

[0031] Prepare the tissue-type plasminogen activator-inhibitor 1 complex with 1% to 5% horse serum albumin solution to make a calibrator and lyophilize it for assignment, the concentration is 50ng / mL

[0032] 2.3. Preparation of reagent 1:

[0033] Take 1 mg tissue-type plasminogen activator-inhibitor 1 complex antibody, and use Tris buffer (pH7.0~8.5) to ultrafilter at 4°C or room temperature; the tissue-type plasminogen activator after ultrafiltration - Add 0.05 mg of biotin to the inhibitor 1 complex antibody, then add Tris buffer (pH 7.0-8.5) to make the final antibody concentration 0.05-1 μg / mL, a...

Embodiment 3

[0042] A method for preparing a t-PAI-C marker detection kit is as follows:

[0043] 3.1. Preparation of calibrator 1:

[0044] The tissue-type plasminogen activator-inhibitor 1 complex was prepared into a calibrator with 1%-5% goat serum albumin solution, freeze-dried, and assigned a concentration of 5 ng / mL.

[0045] 3.2. Preparation of calibrator 2:

[0046] Prepare the tissue-type plasminogen activator-inhibitor 1 complex with 1% to 5% goat serum albumin solution to make a calibrator and lyophilize it for assignment. The prepared concentration is 50ng / mL

[0047] 3.3. Preparation of Reagent 1

[0048] Take 1 mg tissue-type plasminogen activator-inhibitor 1 complex antibody, and ultrafilter it with HEPES buffer (pH7.0-8.5) at 4°C or room temperature; the tissue-type plasminogen activator after ultrafiltration - Add 0.05 mg of biotin to the inhibitor 1 complex antibody, then add HEPES buffer (pH 7.0-8.5) to make the final antibody concentration 0.05-1 μg / mL, and mix for 5...

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Abstract

The invention discloses the technical field related to biological immune in-vitro diagnosis of medical instruments, and particularly relates to a t-PAI-C marker detection kit and a preparation methodthereof. The kit comprises a reagent 1, a reagent 2, a magnetic particle reagent, a calibrator 1 and a calibrator 2 which are independently packaged, and an acridinium ester chemiluminescence technology is combined with immune magnetic particles, so that the kit is high in detection sensitivity and precision, wide in detection range, short in reaction time, economical and effective; a plurality ofsamples can be simultaneously determined on a full-automatic chemiluminiscence instrument, high-throughput rapid determination of a tissue-type plasminogen activator-plasminogen activator inhibitor-1compound is realized, the performance is reliable, the sensitivity is high, the linear range is wide, and the kit can be matched with semi-automatic and full-automatic instruments for use.

Description

Technical field: [0001] The invention relates to the related technical field of in vitro diagnosis of biological immunity of medical devices, in particular to a t-PAI-C marker detection kit and a preparation method thereof. Background technique: [0002] t-PAI-C (tissue plasminogen activator-plasminogen activator inhibitor-1 complex) is a tissue plasminogen activator (tissue plasminogen activator, t- PA) combined with physiological inhibitor PAI-11:1 to form a complex, tissue plasminogen activator-plasminogen activator inhibitor-1 complex is not only a marker of fibrinolytic system activation, but also Markers of vascular endothelial injury. In patients with coronary heart disease, the contents of t-PA and PAI-1 are significantly unbalanced, and are correlated with the severity of the disease. Clinically, tissue plasminogen activator-plasminogen activator inhibitor-1 can The complex judges the prognostic status of the disease. Disseminated intravascular coagulation (DIC) ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/564G01N33/543
CPCG01N33/54326G01N33/564G01N33/6893G01N2333/9726G01N2800/324G01N2800/52
Inventor 颜克亮邹刚军陈文华
Owner 深圳市巨东生物医学工程有限公司
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