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Extrusion blowing method for scattering cells

A cell and dispersal technology, applied in the field of analysis, can solve problems such as low work efficiency

Inactive Publication Date: 2020-12-04
天津速一腾高新技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method of squeezing and blowing for breaking up cells, so as to alleviate the problem of low work efficiency of cell blowing in the prior art by artificial extrusion

Method used

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  • Extrusion blowing method for scattering cells
  • Extrusion blowing method for scattering cells
  • Extrusion blowing method for scattering cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Please also see Figure 1 to Figure 6 , this embodiment provides an extrusion and blowing device for breaking up cells, including a first automatic extrusion module 1 and a second automatic extrusion module 2;

[0038] The first automatic extrusion module 1 includes two extrusion mechanisms 100 that can approach or move away from each other; when the first automatic extrusion module 1 was in an extrusion state, the two extrusion mechanisms 100 approached each other; When the module 1 is in the separated state, the two extrusion mechanisms 100 are far away from each other;

[0039] The second automatic extrusion module 2 includes two extrusion mechanisms 100 that can approach or move away from each other; when the second automatic extrusion module 2 was in an extrusion state, the two extrusion mechanisms 100 approached each other; When the module 2 is in a separated state, the two extrusion mechanisms 100 are far away from each other;

[0040] A gas accumulation chambe...

Embodiment 2

[0067] This embodiment provides a squeeze blowing method for breaking up cells, comprising the following steps:

[0068] S1: gas storage step: the first automatic extrusion module 1 is in the downward extrusion state and the second automatic extrusion module 2 is in the upward extrusion state, so that the first automatic extrusion module 1 and the second automatic extrusion module The two ends of the gas accumulation cavity in the cell blowing tube 3 between the two are closed to accumulate gas;

[0069] S2: deflation step: the first automatic extrusion module 1 maintains the extrusion state and the second automatic extrusion module 2 is in a separated state, and the gas in the gas storage chamber is ejected to the lower end;

[0070] S3: Relaxation step: the first automatic extrusion module 1 changes from the extrusion state to the separation state.

[0071] When it is necessary to perform the blowing operation, the gas storage step is first carried out, the first automatic ...

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Abstract

The invention relates to the technical field of analysis, and particularly relates to an extrusion blowing method for scattering cells, and aims to solve the problem of low working efficiency of a manual extrusion mode adopted for cell blowing in the prior art. The method comprises a first automatic extrusion module and a second automatic extrusion module, wherein the first automatic extrusion module comprises two extrusion mechanisms which can be close to or far away from each other; when the first automatic extrusion module is in an extrusion state, the two extrusion mechanisms are close toeach other and extrude downwards; when the first automatic extrusion module is in a separation state, the two extrusion mechanisms are far away from each other; the second automatic extrusion module comprises two extrusion mechanisms which can be close to or far away from each other; when the second automatic extrusion module is in an extrusion state, the two extrusion mechanisms are close to eachother; and when the second automatic extrusion module is in a separation state, the two extrusion mechanisms are far away from each other. According to the scheme, full-automatic cell blowing can berealized, and the efficiency is high.

Description

technical field [0001] The invention relates to the technical field of analysis, in particular to an extrusion blowing method for breaking up cells. Background technique [0002] At present, pipettes, Pasteur pipettes, pipettes and other utensils are usually used to mix the cells by pipetting. In the specific process of pipetting and mixing, it is necessary to use the front end of such utensils after subtracting the flaring. The most widely used is the Pasteur pipette. [0003] In the step of extracting cells from surgical specimen tissue for culture, it is necessary to repeatedly blow and beat the tissue to make the cells fall off, but the existing blow and blow method usually uses manual extrusion, and the way of repeatedly squeezing the Bartholin tube is inefficient. . Contents of the invention [0004] The object of the present invention is to provide a squeeze blowing method for breaking up cells, so as to alleviate the problem of low work efficiency of cell blowing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/33C12M1/00
CPCC12M47/04
Inventor 鲁春梅
Owner 天津速一腾高新技术有限公司