Serum substitute for cell culture

A serum substitute, cell culture technology, applied in cell culture medium, cell culture active agent, animal cells, etc., can solve the problems of cell inhibition, toxicity, improper use of animal serum, etc., to repair oxidative damage, promote protein and Effects of Nucleic Acid Synthesis

Inactive Publication Date: 2020-12-08
内蒙古奥普赛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, current studies have shown that improper use of animal serum can inhibit cell growth and toxicity, and the composition of animal serum is not very clear, and its components may affect experimental research, which is not conducive to cell metabolism Basic research and research on the effect of specific components on cells

Method used

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  • Serum substitute for cell culture
  • Serum substitute for cell culture
  • Serum substitute for cell culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A serum substitute for cell culture, comprising the following components per liter of serum substitute:

[0039] Alanine 20mg, Aspartic Acid 25mg, Glutamic Acid 60mg, Proline 30mg, Asparagine 15mg, VC 15μM, Vitamin H 70μM, Tocopheryl Acetate 150μM, Tocopherol 80μM, Vitamin A 10μM, Bovine Pituitary Extract 15g , insulin-like growth factor I 80μg, catalase 250μg, human recombinant insulin 450μg, human transferrin 5000μg, superoxide dismutase 5000000U, corticosterone 5mM, D-galactose 150000mM, ethanolamine hydrochloride 100mM, glutathione 49mM , L-carnitine hydrochloride 80mM, linoleic acid 1mM, progesterone 0.5mM, putrescine hydrochloride 150mM, sodium selenite 0.5mM, ferric citrate 0.8mg, CaCl 2 .2H 2 O140mg, CuSO 4 .5H 2 O0.001mg and FeSO 4 .7H 2 O0.1mg and Pluronic F-685g.

Embodiment 2

[0041] A serum substitute for cell culture, including the following components per liter of serum substitute: 25 mg of alanine, 30 mg of aspartic acid, 70 mg of glutamic acid, 40 mg of proline, 20 mg of asparagine, 25 μM of VC, vitamin H110μM, tocopheryl acetate 200μM, tocopherol 120μM, vitamin A15μM, bovine pituitary extract 25g, insulin-like growth factor I120μg, catalase 350μg, human recombinant insulin 550μg, human transferrin 10000μg, superoxide dismutase 5000000U, corticosterone 20mM, D-galactose 250000mM, ethanolamine hydrochloride 150mM, glutathione 165mM, L-carnitine hydrochloride 120mM, ferric citrate 1.2mg, CaCl 2 .2H 2 O160mg, CuSO 4 .5H 2 O0.003mg, FeSO 4 .7H 2 O0.2mg, linoleic acid 10mM, progesterone 1mM, putrescine hydrochloride 250mM, sodium selenite 1mM and Pluronic F-6815g

Embodiment 3

[0043] A serum substitute for cell culture, including the following components per liter of serum substitute: alanine 22mg, asparagine 19mg, aspartic acid 26.4mg, glutamic acid 66mg, proline 35.2mg, VC 20 μM, vitamin H 100 μM, tocopheryl acetate 185 μM, tocopherol 100 μM, vitamin A 14 μM, bovine pituitary extract 20 g, insulin-like growth factor I 100 μg, catalase 300 μg, human recombinant insulin 500 μg, human transferrin 6000 μg, superoxide Substance dismutase 5000000U, corticosterone 10mM, D-galactose 200000mM, ethanolamine hydrochloride 120mM, glutathione 100mM, L-carnitine hydrochloride 100mM, linoleic acid 5mM, progesterone 0.63mM, putrescine hydrochloride 200mM, Sodium Selenite 0.52mM, Ferric Citrate 1mg, CaCl 2 .2H 2 O154.4mg, CuSO 4 .5H 2 O0.002mg, FeSO 4 .7H 2 O 0.147mg and Pluronic F-68 10g.

[0044] The preparation method of the above-mentioned serum substitute is as follows:

[0045] 1) According to the proportion, weigh alanine, asparagine, aspartic acid, ...

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Abstract

The invention discloses a serum substitute for cell culture. Each liter of the serum substitute comprises the following components: 140-165 mg of amino acid, 15-20 mg of asparagine, 15-25 [mu] M of VC, 70-110 [mu] M of vitamin H, 150-200 [mu] M of tocopheryl acetate, 80-120 [mu] M of tocopherol, 10-15 [mu] M of vitamin A, 15-25 g of a bovine pituitary extract, 80-120 [mu] g of an insulin-like growth factor I, 250-350 [mu] g of catalase, 450-550 [mu] M of insulin human recombinant, 5000-10000 [mu] M of human transferrin, 5000000U of superoxide dismutase, 5mM-20mM of corticosterone, 150000-250000mM of D-galactose, 100-150mM of diethanolamine hydrochloride, 49-165mM of glutathione, 80-120mM of l-carnitinechloride, 140.901-161.403mg of inorganic salts and 5-15g of Pluronic F-68.

Description

technical field [0001] The invention relates to the field of bioengineering, and more specifically relates to a serum substitute for cell culture. Background technique [0002] Animal serum is the most used natural medium in cell culture. It is rich in nutrients necessary for cell growth and is often used for in vitro culture of animal cells. It mainly includes fetal bovine serum, calf serum, adult bovine serum, horse serum, goat serum, chicken serum, rabbit serum, etc. Among them, fetal bovine serum is the most commonly used. Animal serum provides growth factors, hormones, transfer proteins, factors for attachment and spreading on the culture substrate, protease inhibitors and other nutrients required for cell proliferation in cell culture. The advantage of using serum is that serum contains most of the factors that promote cell proliferation and maintenance, and it is almost a universal growth supplement, suitable for cell culture of animal, human and insect cells, etc. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00C12N5/077
CPCC12N5/0037C12N5/0656C12N2500/32C12N2500/38C12N2500/84C12N2501/105C12N2500/25C12N2500/34C12N2500/46C12N2501/71C12N2501/30C12N2500/14C12N2500/20C12N2501/999
Inventor 赵峻岭
Owner 内蒙古奥普赛生物科技有限公司
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