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Composition and kit for early detection of cervical high-grade lesions and cervical cancer

An early detection, high-level technology used in the field of nucleic acid in vitro diagnostics

Active Publication Date: 2020-12-08
BEIJING ORIGIN-POLY BIO-TEC CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] The purpose of the present invention is to provide a composition and kit for early detection of cervical high-grade lesions and cervical cancer, which solves the problem that false positive results are prone to occur in conventional detection

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  • Composition and kit for early detection of cervical high-grade lesions and cervical cancer
  • Composition and kit for early detection of cervical high-grade lesions and cervical cancer
  • Composition and kit for early detection of cervical high-grade lesions and cervical cancer

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Embodiment Construction

[0061] In order to enable those skilled in the art to better understand the solutions of the present invention, the present invention will be further described in detail below in conjunction with specific embodiments.

[0062] This patent involves three markers related to cervical cancer:

[0063]The FAM19A4 (family with sequence similarity 19member A4) gene is a member of the TAFA gene family; the TAFA gene family mainly encodes small molecular proteins, which are related to stress and inflammation. FAM19A4 is a ligand of formyl peptide receptor 1, which can promote phagocytosis and increase the release of reactive oxygen species from macrophages. Usually upregulated in lipopolysaccharide-stimulated monocytes and macrophages. In recent years, some research reports have shown that FAM19A4 is closely related to cervical cancer. It is an important marker of cervical cancer and an important means of triage for patients with high-risk HPV infection in cervical cancer screening. ...

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Abstract

The invention discloses a composition and kit for early detection of cervical high-grade lesions and cervical cancer. The composition for early detection of cervical high-grade lesions and cervical cancer comprises an FAM19A4 gene methylation primer, a probe corresponding to a methylation site and a methylation blocking primer, a JAM3 gene methylation primer, a probe corresponding to the methylation site and a methylation blocking primer, a PAX1 gene methylation primer, a probe corresponding to the methylation site and the methylation blocking primer, and a pair of primers of an internal reference gene GAPDH and a probe corresponding to the methylation site. According to the method, the methylation sites of FAM19A4, JAM3 and PAX1 genes are accurately detected by adopting multiple multi-channel fluorescence and blocking technical means, accurately identifying specific primer probes and methylation sequences, fully releasing a methylation template under the action of a plurality of blocking primers and optimizing special methylated DNA polymerase.

Description

technical field [0001] The invention belongs to the technical field of nucleic acid in vitro diagnosis, and specifically relates to a composition and a kit for applying specific gene methylation markers to cervical high-grade lesions and early detection of cervical cancer. Background technique [0002] At present, cervical cancer is the fourth most common cancer among women in the world. According to statistics, there were 570,000 new cases of cervical cancer and 311,000 deaths in 2018. Cervical cancer has become the main cause of cancer death among women in most low- and middle-income countries. The etiology of cervical cancer is clear, and persistent infection of high-risk human papillomavirus (HPV) is a necessary condition. The process of developing from cervical precancerous lesions to invasive cervical cancer can take up to ten years, so early cervical cancer screening can Effectively block the progression of precancerous lesions. In recent years, the incidence of cerv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6858C12N15/11
CPCC12Q1/6886C12Q1/6858C12Q2600/154C12Q2600/166C12Q2600/16C12Q2531/113C12Q2537/143C12Q2561/101C12Q2563/107C12Q1/6816C12Q1/686C12Q2537/164C12Q1/6848
Inventor 刘沛王林海王紫芸
Owner BEIJING ORIGIN-POLY BIO-TEC CO LTD