Composition and kit for early detection of cervical high-grade lesions and cervical cancer
A technology for detecting reagents and compositions, applied in the field of compositions and kits for early detection of cervical high-grade lesions and cervical cancer, to achieve the effects of improving sensitivity, intuitive interpretation, and avoiding cross-contamination
Active Publication Date: 2021-11-26
BEIJING ORIGIN-POLY BIO-TEC CO LTD
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Problems solved by technology
[0010] The purpose of the present invention is to provide a composition and kit for early detection of cervical high-grade lesions and cervical cancer, which solves the problem that false positive results are prone to occur in conventional detection
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[0061] In order to enable those skilled in the art to better understand the solutions of the present invention, the present invention will be further described in detail below in conjunction with specific embodiments.
[0062] This patent involves three markers related to cervical cancer:
[0063]The FAM19A4 (family with sequence similarity 19member A4) gene is a member of the TAFA gene family; the TAFA gene family mainly encodes small molecular proteins, which are related to stress and inflammation. FAM19A4 is a ligand of formyl peptide receptor 1, which can promote phagocytosis and increase the release of reactive oxygen species from macrophages. Usually upregulated in lipopolysaccharide-stimulated monocytes and macrophages. In recent years, some research reports have shown that FAM19A4 is closely related to cervical cancer. It is an important marker of cervical cancer and an important means of triage for patients with high-risk HPV infection in cervical cancer screening. ...
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Abstract
The invention discloses a composition and a kit for early detection of cervical high-grade lesions and cervical cancer. The composition for early detection of cervical high-grade lesions and cervical cancer includes FAM19A4 gene methylation primers and methylation sites Corresponding probes and methylation blocking primers, JAM3 gene methylation primers, probes corresponding to methylation sites and methylation blocking primers, PAX1 gene methylation primers, probes corresponding to methylation sites And the methylation blocking primer, a pair of primers of the internal reference gene GAPDH and the probe corresponding to the methylation site. Using multiple multi-channel fluorescence and blocking technology, through the accurate identification of specific primer probes and methylated sequences, coupled with the role of multiple blocking primers to fully release the methylated template, and optimized special methylated DNA Polymerase, accurate detection of methylation sites of FAM19A4, JAM3 and PAX1 genes.
Description
technical field [0001] The invention belongs to the technical field of nucleic acid in vitro diagnosis, and specifically relates to a composition and a kit for applying specific gene methylation markers to cervical high-grade lesions and early detection of cervical cancer. Background technique [0002] At present, cervical cancer is the fourth most common cancer among women in the world. According to statistics, there were 570,000 new cases of cervical cancer and 311,000 deaths in 2018. Cervical cancer has become the main cause of cancer death among women in most low- and middle-income countries. The etiology of cervical cancer is clear, and persistent infection of high-risk human papillomavirus (HPV) is a necessary condition. The process of developing from cervical precancerous lesions to invasive cervical cancer can take up to ten years, so early cervical cancer screening can Effectively block the progression of precancerous lesions. In recent years, the incidence of cerv...
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IPC IPC(8): C12Q1/6886C12Q1/6858C12N15/11
CPCC12Q1/6886C12Q1/6858C12Q2600/154C12Q2600/166C12Q2600/16C12Q2531/113C12Q2537/143C12Q2561/101C12Q2563/107C12Q1/6816C12Q1/686C12Q2537/164C12Q1/6848
Inventor 刘沛王林海王紫芸
Owner BEIJING ORIGIN-POLY BIO-TEC CO LTD