A carbon catabolism regulatory protein ccpa mutant i42a

A technology for catabolism and regulation of proteins, applied in the field of recombinant expression vectors and recombinant microbial cells, it can solve the problems of destroying the metabolic pathways of bacteria and restricting the growth of bacteria, and achieve the effect of reducing preference and changing the phenomenon of carbon catabolism repression.
CN112062822BActive Publication Date: 2022-02-01JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2022-02-01

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Abstract

The invention discloses a carbon catabolism regulating protein CcpA mutant I42A, which belongs to the technical field of protein engineering. The present invention mutates the CcpA gene cloned from the Bacillus licheniformis genome to obtain a carbon catabolism regulatory protein CcpA mutant I42A, the amino acid sequence of which is shown in SEQ ID NO.2, and the nucleotide sequence of the gene encoding it As shown in SEQ ID NO.1. The present invention has constructed the recombinant expression vector comprising the above-mentioned CcpA mutant I42A gene, and transformed the constructed expression vector into the Bacillus licheniformis CcpA gene deficient strain to obtain the recombinant Bacillus licheniformis; the obtained recombinant Bacillus licheniformis can obviously change the Carbon catabolism repression in the presence of glucose reduces xylose preference in Bacillus licheniformis.
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Description

technical field

[0001] The invention belongs to the field of protein engineering, relates to a carbon catabolism regulating protein CcpA mutant I42A, also relates to a recombinant expression vector and a recombinant microbial cell expressing the mutant, and further relates to the effect of the mutant on Dangwood in the fermentation process of Bacillus licheniformis Effects of sugar on xylose utilization when co-existing with glucose. Background technique

[0002] Bacillus licheniformis (Bacillus licheniformis) is a Gram-positive bacterium with the advantages of heat resistance, rich enzyme system, high enzyme production, moderate growth rate, protein folding, and genome-wide information disclosure. Compared with Escherichia coli, Bacillus licheniformis has the advantages of high heat resistance, low pH tolerance, and high biomass. Therefore, it is not only widely used as a host for exogenous gene expression, but also has great potential in the fermentation industry. potenti...

Claims

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