A carbon catabolism regulatory protein CCPA mutant K31A

A technology for catabolism and regulation of proteins, which is applied in the field of carbon catabolism regulation protein CcpA mutant K31A, recombinant expression vectors and recombinant microbial cells, which can solve the problems of destroying bacterial metabolic pathways and restricted bacterial growth, so as to reduce preference sex, altering the effect of carbon catabolism repression
CN112062821BActive Publication Date: 2022-02-01JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2022-02-01

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Abstract

The invention discloses a carbon catabolism regulating protein CcpA mutant K31A, which belongs to the technical field of protein engineering. The present invention mutates the CcpA gene cloned from the Bacillus licheniformis genome to obtain a carbon catabolism regulatory protein CcpA mutant K31A, whose amino acid sequence is shown in SEQ ID NO.2, and the gene nucleotide sequence encoding it is shown in Shown in SEQ ID NO.1. The present invention constructs a recombinant expression vector containing the above-mentioned CcpA mutant K31A gene, and transforms the constructed expression vector into a CcpA gene-deficient strain of Bacillus licheniformis to obtain the recombinant Bacillus licheniformis. The obtained recombinant bacillus licheniformis can obviously change the phenomenon of carbon catabolism repression caused by the presence of glucose, and can reduce the preference of the bacillus licheniformis to xylose.
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Description

Technical field

[0001] The present invention belongs to the field of protein engineering, and involves a carbon decomposition metabolic regulatory protein CCPA mutant K31A, and further relates to recombinant expression vectors expressing the mutant and recombinant microbial cells, and further relates to the mutant. Effects of Sugar and Glucose Co - co - co - co - co - co - co - co - co - co - coordination. Background technique

[0002] Bacillus licheniformis is a Gram-positive bacteria, which has the advantages of heat resistance, rich enzyme, high enzyme, moderate growth rate, moderate protein fold, and all genome information disclosure. Compared with E. coli, the bacillus undergarment has high heat resistance, low pH tolerance, high biomass, not only used for expression hosts of exogenous genes, but also has huge in the fermentation industry. potential.

[0003] As a high-quality carbon source, xylose can be obtained by hydrolysis of lignocellulose, with the current environment...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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