Carbon catabolism regulation protein CcpA mutant I42A

A technology for catabolism and protein regulation, which is applied in the field of recombinant expression vectors and recombinant microbial cells, and can solve the problems of destroying bacterial metabolic pathways and restricting bacterial growth.
CN112062822AActive Publication Date: 2020-12-11JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2020-12-11

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Abstract

The invention discloses a carbon catabolism regulation protein CcpA mutant I42A, and belongs to the technical field of protein engineering. A CcpA gene cloned from a bacillus licheniformis genome is mutated to obtain a carbon catabolism regulation protein CcpA mutant I42A, wherein the amino acid sequence of the mutant I42A is as shown in SEQ ID NO.2, and the nucleotide sequence of the gene for encoding the mutant I42A is as shown in SEQ ID NO.1. According to the invention, a recombinant expression vector containing the CcpA mutant I42A gene is constructed, and the constructed expression vectoris transformed into a bacillus licheniformis CcpA gene defective strain to obtain recombinant bacillus licheniformis. The obtained recombinant bacillus licheniformis can obviously change the carbon catabolism repression phenomenon caused by the existence of glucose, and can reduce the preference of bacillus licheniformis to xylose.
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Description

technical field

[0001] The invention belongs to the field of protein engineering, relates to a carbon catabolism regulating protein CcpA mutant I42A, also relates to a recombinant expression vector and a recombinant microbial cell expressing the mutant, and further relates to the effect of the mutant on Dangwood in the fermentation process of Bacillus licheniformis Effects of sugar on xylose utilization when co-existing with glucose. Background technique

[0002] Bacillus licheniformis (Bacillus licheniformis) is a Gram-positive bacterium with the advantages of heat resistance, rich enzyme system, high enzyme production, moderate growth rate, protein folding, and genome-wide information disclosure. Compared with Escherichia coli, Bacillus licheniformis has the advantages of high heat resistance, low pH tolerance, and high biomass. Therefore, it is not only widely used as a host for exogenous gene expression, but also has great potential in the fermentation industry. potenti...

Claims

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