Plant endophytic fungus Setophoma terrestris D2G24 and application thereof

A plant endophytic fungus, D2G24 technology, applied in plant growth regulators, plant growth regulators, applications, etc., can solve problems such as hindering plant root respiration and absorption of water and nutrients, cell membrane damage, phytotoxicity, etc. Oxidizing ability, low cost, obvious effect

Active Publication Date: 2020-12-18
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Excessive heavy metal concentration in the soil will also hinder the respiration of plant roots and make it difficult to absorb water and nutrients from the soil, thereby affecting the growth and development of plants, making it impossible for agricultural applications in areas polluted by heavy metals, and heavy metals in soil and water in plant growth Continuous enrichment in plants during the development process will have significant toxic effects on plants, such as reduction of plant growth, cell membrane damage, oxidative toxicity, etc.

Method used

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  • Plant endophytic fungus Setophoma terrestris D2G24 and application thereof
  • Plant endophytic fungus Setophoma terrestris D2G24 and application thereof
  • Plant endophytic fungus Setophoma terrestris D2G24 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Isolation, screening and identification of Echinococcus solitaryus D2G24

[0029] A. Collect the plant samples of Nepeta nepeta, the dominant plant in the slag area of ​​the heavy metal polluted land, and rinse them under tap water;

[0030] B. Divide the plant sample into three parts: roots, stems and leaves, and carry out surface disinfection respectively. First, soak in ethanol solution with a volume concentration of 75% for 3 to 5 minutes, rinse with sterile water for 3 to 5 times, and then use available chlorine with a concentration of 5%. Soak in sodium hypochlorite solution for 2 to 3 minutes, rinse with sterile water for 3 to 5 times, and place it on sterile filter paper to absorb the water after rinsing; cut the root, stem, and leaf tissue pieces into fragments and paste them on a tissue containing 0.5g / Cultivate on the PDA medium of L streptomycin sulfate and 0.5g / L penicillin, culture at 24-26°C for 40-50 days, observe the next day, pick if there ...

Embodiment 2

[0037] Example 2: Research on the Effect of Echinococcus solitaryus D2G24 on the Accumulation of Low Concentration Heavy Metals

[0038] This example aims to prove that the filamentous fungus D2G24 provided by the present invention promotes plant growth and plant heavy metal pollution restoration in heavy metal pollution; Dysphania ambrosioides ) is the test plant, and the experimental process is as follows:

[0039] A. Preparation of sterile seedlings of Nepeta nepeta: The seed samples of Nepeta nepeta were collected on August 10, 2018 at the abandoned slag pile next to Sanduoduo Village, Zhehai Town, Huize County (26°28′17″ north latitude, 103 east longitude °37′34″, altitude 2273 m), and stored at 4°C for future use. Randomly select several Nepeta seeds from soil, and carry out surface disinfection according to the following procedures: first soak in 75% ethanol solution for 3 minutes, rinse with sterile water for 4 times; then soak in NaClO solution with an available chl...

Embodiment 3

[0054] Embodiment 3: the influence of Acanthosporium acanthus D2G24 on the growth of Nepeta nepeta

[0055] Experimental process and technical method are the same as embodiment 2, and difference is during the growth period of Nepeta nepeta, increased the mensuration of nepeta seedling biomass; Transplanting nepeta nepeta seedlings harvested the 46th day, measured strain Height, root length, dry weight, MDA, chlorophyll, glutathione GSH, etc.; GSH=T-GSH-2GSSG to calculate GSH content;

[0056] Pot experiment results are shown in Table 3 and figure 2 The results showed that the inoculation of Echinacea solitaryus D2G24 significantly promoted the growth of Nepeta nepeta under heavy metal stress; compared with the control group, the treatment group inoculated with D2G24 increased the plant height, root length and dry weight of Nepeta nepeta respectively. Content, plant height, T-GSH (total glutathione), GSH (glutathione), GSSH (oxidized glutathione) and MDA (malondialdehyde) con...

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Abstract

The invention discloses an Setophoma terrestris D2G24 strain. The preservation number of the Setophoma terrestris D2G24 strain in the China General Microbiological Culture Collection Center is CGMCC (China General Microbiological Culture Collection Center) No. 20268. The strain is applied to bioremediation of heavy metals lead, zinc and cadmium. Under the stress of heavy metal pollution, the strain can promote plant growth, promote the transfer capacity of plants to heavy metals, has relatively strong accumulation capacity to heavy metals cadmium and zinc, and is suitable for in-situ remediation of large-area heavy metal cadmium and zinc pollution.

Description

technical field [0001] The invention belongs to the field of environmental management, and in particular relates to a plant endophytic fungus Acanthosporium aeruginosa ( Setophoma terrestris ) D2G24 and its application in bioremediation of heavy metal lead, zinc, cadmium pollution. Background technique [0002] With the rapid development of the economy, more and more heavy metals such as cadmium (Cd), lead (Pb), chromium (Cr), mercury (Hg) and metalloid arsenic (As) with significant biological toxicity are released into the environment. , resulting in a serious deterioration of the domestic environmental quality. As the most basic and main component of the environmental system, soil is an indispensable and important resource for human survival. However, due to the dual effects of natural factors and human activities, the problem of soil heavy metal pollution is becoming more and more serious. Excessive heavy metal concentration in the soil will also hinder the respiratio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/30A01P21/00B09C1/10C12R1/645
CPCC12N1/14A01N63/30B09C1/105C12R2001/645C12N1/145
Inventor 李海燕汤雯婷高永涵李绍仕江悦娟毛文沁
Owner KUNMING UNIV OF SCI & TECH
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