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Application of ethyl acetate extract of Delphinium in controlling Plutella xylostella
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A technology of ethyl acetate and ethyl acetate, applied in the application, animal repellent, plant growth regulator and other directions, to achieve the effects of easy degradation, environmental safety, and resistance to drugs
Active Publication Date: 2021-08-24
ANHUI AGRICULTURAL UNIVERSITY
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Problems solved by technology
[0006] Aiming at the problem that the existing delphinium ethyl acetate extract has not been applied to the control of diamondback moth, the present invention provides the application of delphinium ethyl acetate extract in the prevention and treatment of diamondback moth. Remarkable insecticidal effect
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Embodiment 1
[0023] Contact killing effect of delphinium ethyl acetate extract on Plutella xylostella as a contact killer
[0024] 1. Insects to be tested
[0025] Plutella xylostella: provided by the insect culture room of Anhui Agricultural University. The diamondback moth was reared in an incubator with a temperature of 25°C, a relative humidity of 75%-80%, and a light condition of L:D=16:8h. Healthy third-instar larvae with consistent individual development were selected for testing.
[0026] 2. Delphinium ethyl acetate extract for testing
[0027] The delphinium ethyl acetate extract can be obtained by operating according to the following method.
[0028] Delphinium seeds were purchased from Bozhou Traditional Chinese Medicine Market. Wash the delphinium seeds with clean water, drain them, and dry them in an oven at low temperature; crush the dried seeds with a pulverizer and sieve them; soak them in ethyl acetate solvent according to the ratio of powder: solvent = 1:10 , ultraso...
Embodiment 2
[0035] The gastrotoxic effect of delphinium ethyl acetate extract on Plutella xylostella as a gastrotoxic agent
[0036] 1. Insects to be tested
[0037] Plutella xylostella: provided by the insect culture room of Anhui Agricultural University. The diamondback moth was reared in an incubator at a temperature of 25°C, a relative humidity of 75%-80%, and a light condition of L:D=16:8h. Healthy third-instar larvae with consistent individual development were selected for testing.
[0038] 2. Delphinium ethyl acetate extract for testing
[0039] The delphinium ethyl acetate extract can be obtained by operating according to the following method.
[0040] Delphinium seeds were purchased from Bozhou Traditional Chinese Medicine Market. Wash the delphinium seeds with clean water, drain them, and dry them in an oven at low temperature; grind the dried seeds with a grinder and sieve them; soak them in ethyl acetate solvent according to the ratio of powder: solvent = 1:10 , ultrasoni...
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Abstract
The invention discloses the application of delphinium ethyl acetate extract in preventing and treating diamondback moth, and belongs to the technical field of biological pesticides. The species of diamondback moth described therein is Plutella xylostella (L.), and the concentration of the delphinium ethyl acetate extract is 3.125mg / ml-50mg / ml, specifically, the delphinium ethyl acetate extract is used as contacticide to control diamondback moth. The invention adopts the delphinium ethyl acetate extract for the first time to prevent and control diamondback moth, and tests show that it has remarkable insecticidal effect on vegetable pest diamondback moth. Specifically, the prepared contacticide uses delphinium seeds as raw materials and is prepared by ultrasonic extraction, which shows a good contact effect on diamondback moth, and is an ideal botanical pesticide for killing diamondback moth in agriculture, and the source of the extract is As for the plant itself, it is easy to degrade in nature, safe for the environment, and it is difficult for pests to develop resistance.
Description
technical field [0001] The invention belongs to the technical field of biopesticides, and in particular relates to the application of delphinium ethyl acetate extract in controlling diamondback moth. Background technique [0002] The diamondback moth (Plutella xylostella) is a pest of the Lepidoptera Plutella family, which mainly harms cruciferous vegetables such as cabbage, Chinese cabbage, and radish. Plutella xylostella larvae often gather in the heart leaves to feed on the mesophyll, causing holes and nicks in the leaves. When the damage is serious, the whole leaf is eaten into a net, which affects the commerciality of vegetables. For a long time in my country, the control of diamondback moth has mainly relied on chemical pesticides. Excessive reliance on a single prevention and control method has caused many problems. For example, the resistance of diamondback moth is increasing year by year. threaten. At the same time, with the development of social economy and the im...
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Application Information
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