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Application of hsa_circ_0069922 as a marker

A drug and peripheral blood technology, applied in the clinical screening of SAI, biomedicine and molecular biology, can solve the problems of increased expression, aggravated stroke damage, etc., achieving good sensitivity and specificity, and high diagnostic value.

Active Publication Date: 2022-06-24
NANJING FIRST HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, circHECTD1 can compete with miR-142, leading to an increase in the expression of the downstream target gene TIPARP of miR-142, causing the activation of astrocytes and aggravating stroke damage.

Method used

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  • Application of hsa_circ_0069922 as a marker
  • Application of hsa_circ_0069922 as a marker
  • Application of hsa_circ_0069922 as a marker

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Experimental program
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Embodiment

[0024] The genetic research group of the present invention passed SAI screening in the early stage, selected 5 pairs of SAI and PBMCs of stroke uninfected patients for high-throughput sequencing, and screened out circRNA molecules with high expression differences for screening verification analysis. The present invention selects hsa_circ_0069922, which is used for SAI screening through subsequent verification and statistical analysis.

[0025] Research subjects and samples

[0026] A total of 60 SAI and uninfected patients admitted to Nanjing First Hospital from December 2018 to March 2020 were selected, including 30 SAI and 30 uninfected patients, all confirmed by imaging examination, before treatment and during treatment Use EDTA anticoagulation tube to obtain 3ml-5ml of human peripheral blood, take appropriate methods to store and process samples within 2h (to avoid RNA degradation).

[0027] PBMCs isolation

[0028] Take the peripheral blood and transfer it to a 15ml cen...

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Abstract

The present invention discloses the application of hsa_circ_0069922 as a marker in the preparation of products for screening stroke-related infections. That is, the products used for the screening of stroke-related infections include reagents or drugs for detecting the expression level of hsa_circ_0069922 in single nucleated cells of peripheral blood of patients. The new molecular marker hsa_circ_0069922 developed by the present invention for the screening and diagnosis of SAI provides a new reference for the screening of SAI; the diagnostic value of the marker hsa_circ_0069922 described in the present invention is high, and ROC curve analysis shows that it can better distinguish the When examining SAI patients, the area under the curve is 0.832, which has good sensitivity and specificity, and is expected to become an important indicator for the clinical diagnosis and treatment of SAI.

Description

technical field [0001] The invention relates to the application of hsa_circ_0069922 as a marker in the preparation of products or drugs for stroke-related infection screening, belonging to the technical field of biomedicine and molecular biology, and in particular to the field of clinical screening of SAI. Background technique [0002] As a global public health problem, stroke not only causes permanent disability, but also is the second leading cause of human death. In the past 30 years, the incidence of stroke in my country has risen sharply, and it has become the first cause of death in our country. Stroke includes ischemic stroke and hemorrhagic stroke, among which ischemic stroke accounts for about 85% of all strokes. Although the treatment of ischemic stroke has developed rapidly in recent years, there are still some unresolved clinical problems in the treatment of ischemic stroke, among which stroke-associated infection (SAI) has attracted worldwide attention. SAI is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12N15/11A61K45/00A61P31/00A61P9/10A61P25/00
CPCC12Q1/6883A61K45/00A61P31/00A61P9/10A61P25/00C12Q2600/118C12Q2600/158C12Q2600/166C12Q2600/178
Inventor 邓齐文周俊山施洪超孙慧玲李烁
Owner NANJING FIRST HOSPITAL
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