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A strain of Bacillus Velez and its application in the control of plant downy mildew

A technology of Bacillus velei and Bacillus, applied in the direction of application, plant growth regulator, and microorganism-based methods, to achieve stable control effect, simple fermentation process, and low production cost

Active Publication Date: 2022-02-18
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, at present, there is no report that Bacillus Veles is used to control cucumber downy mildew both at home and abroad.

Method used

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  • A strain of Bacillus Velez and its application in the control of plant downy mildew
  • A strain of Bacillus Velez and its application in the control of plant downy mildew
  • A strain of Bacillus Velez and its application in the control of plant downy mildew

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Isolation and screening of antagonistic bacterial strains

[0042] 1. Isolation and primary screening of strains

[0043] (1) Isolation of antagonistic strains

[0044] In July 2019, tomato roots with root-knot nematode root knots were collected in He'an Village, Liugezhuang Town, Haiyang City, Shandong Province. The tomato roots with root knots weighing about 1 gram were sterilized on the surface, and then cut into pieces with sterilized scissors , add 1 mL of sterilized water, grind with a sterilized mortar and pestle, separate the bacterial strains from the grinding juice by the dilution plate method, and the medium is LB solid medium. After the colonies grow, pick a single bacterial colony on LB solid plates were streaked and purified and preserved.

[0045] (2) Preparation of bacterial fermentation supernatant

[0046] Streak the bacterial strains preserved on the slant on the LB solid medium, culture at 30°C for 24 hours, transfer the cultured colony ...

Embodiment 2

[0072] Example 2 Identification of cucumber downy mildew antagonistic bacterial strain HMQAU19044

[0073] 1. Morphological identification

[0074] Transfer the strain HMQAU19044 on the slant to the LB medium plate, draw a three-zone line, and culture it in a 30°C incubator for 48 hours. After the colony has grown into a single colony, observe the colony shape and take pictures, including the colony shape, size, and color. , Surface, Edge, Transparency, Bump Shape. Bacterial strains cultured for 24 hours were picked for Gram staining microscope examination, and the method was referred to "Handbook of Common Bacterial System Identification".

[0075] Strain HMQAU19044 was cultured on LB solid medium at 30°C for 2 days. The diameter of the colony was 2.5-5mm. Produce spores, which are oval in shape. Such as figure 1 shown.

[0076] 2. Physiological and biochemical identification

[0077] Referring to the "Common Bacterial System Identification Manual", the strain HMQAU1904...

Embodiment 3

[0089] Example 3 Inhibitory properties of bacterial strain HMQAU19044 on different developmental stages of cucumber downy mildew

[0090] 1. Preparation of strain HMQAU19044 and its bacterial agent

[0091] Solid LB medium is prepared by the following method: Take 10g tryptone, 10g sodium chloride, 5g yeast extract juice, 15-20g agar powder, dissolve in deionized water, pH6.8, dilute to 1L with deionized water, 121℃ Moist heat sterilization for 25 minutes.

[0092] The liquid LB medium was prepared by the following method: take 10g tryptone, 10g sodium chloride, 5g yeast extract juice, dissolve in deionized water, pH6.8, dilute to 1L with deionized water, and sterilize with damp heat at 121°C for 25min.

[0093] Activated strain: pick a ring of colonies, inoculate in 50mL liquid LB medium, shake at 200rpm, 30°C for 24h to make seed solution.

[0094] Preparation of fermentation broth: 1 / 50 of the prepared seed solution was inoculated in 50 mL of liquid LB medium, 200 rpm, 30...

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Abstract

The present invention provides a Bacillus velezensis strain HMQAU19044, which was deposited in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures on January 19, 2020, with a deposit registration number of CGMCC No.19420. Bacillus velezensis (Bacillus velezensis) strain HMQAU19044 provided by the present invention has significant control effect to cucumber downy mildew, and the research of embodiment shows, bacterial strain HMQAU19044 fermentation filtrate has obvious inhibition to the release of cucumber downy mildew zoospore Effect; the fermentation liquid of strain HMQAU19044 has obvious inhibitory effect on the release of zoospores of cucumber downy mildew; To a certain extent, the lesion expansion of the infected cucumber leaves is inhibited; the fermentation filtrate and fermentation liquid of the strain HMQAU19044 of the present invention have a good control effect on cucumber downy mildew.

Description

technical field [0001] The invention belongs to the technical field of biological control of plant diseases, and in particular relates to a strain of Bacillus velezensis and its application in preventing and controlling plant downy mildew diseases. Background technique [0002] Cucumber is one of the important staple vegetables in our country, and the occurrence of downy mildew leads to severe losses of cucumbers cultivated in open fields and protected areas all year round. Cucumber downy mildew is a serious disease caused by pseudo downy mildew in Cuba. Cucumber downy mildew is highly prevalent, has a fast transmission speed and a high incidence rate. If the environmental conditions are suitable, once it occurs in only 1 to 2 weeks, all the leaves of the cucumber plant will wither and die, and the cucumber fruit will be directly affected. . Under normal circumstances, downy mildew can lead to a 30% to 50% reduction in cucumber production, and in severe cases, the producti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/07
CPCC12N1/20A01N63/22C12R2001/07C12N1/205A01P3/00
Inventor 梁晨肖倩王博李守望
Owner QINGDAO AGRI UNIV
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