Full-automatic nucleic acid extraction hybridization capture and detection device based on magnetic separation

Abstract

The invention relates to an integrated full-automatic nucleic acid extraction liquid-phase hybridization capture and detection device. The device integrates the following core modules of a nucleic acid pretreatment module, a reagent storage module, a nucleic acid hybridization module, a magnetic separation module, a pipetting module and a detection module. All the modules run around task flows ofnucleic acid extraction, nucleic acid denaturation, nucleic acid hybridization, signal generation and detection, and finally the purpose of full-automatic nucleic acid liquid-phase hybridization capture and detection is achieved.

Description

technical field [0001] The invention relates to an integrated fully automatic liquid phase hybridization capture detection device after nucleic acid extraction, which integrates the following core modules: nucleic acid pretreatment module, reagent storage module, nucleic acid hybridization module, magnetic separation module, pipetting module, detection module. All modules revolve around: nucleic acid extraction, nucleic acid denaturation, nucleic acid hybridization, signal generation and detection task flow operation, and finally realize the goal of fully automatic nucleic acid liquid phase hybridization capture detection. Background technique [0002] Nucleic acid hybridization detection is one of the earliest nucleic acid detection methods. The principle is the theory of nucleic acid denaturation and renaturation. During the renaturation process of denatured nucleic acid, complementary nucleotide sequences (DNA and DNA, DNA and RNA, RNA and RNA, etc.) form non-consensus s...

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Problems solved by technology

In wider fields such as infectious diseases and clinical microbial identification, there is no mature development and application of nucleic acid hybridization detection products.

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