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A Method of Using Digoxigenin-labeled Oligonucleotide Centromere Probes for In Situ Hybridization

A technology of digoxigenin labeling and oligonucleotide, applied in the field of molecular biology nucleic acid labeling, can solve the problems of many failures and complicated steps.

Pending Publication Date: 2021-04-20
GUANGZHOU EXONS BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

It is recognized that the traditional nucleic acid labeling uses the 5-end and 3-end of the nucleic acid, random primers, oligonucleotide synthesis, etc., but the steps are cumbersome, and most laboratories fail many times in labeling experiments, and cannot complete the probe well mark

Method used

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  • A Method of Using Digoxigenin-labeled Oligonucleotide Centromere Probes for In Situ Hybridization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] 1. The preparation process of the probe labeling solution:

[0015] A Solution: 0.2mol / L NaHCO 3 (pH8.7)

[0016] First prepare 0.1mol / L Na 2 CO 3 Solution: Weigh 5.3g of sodium carbonate powder with an analytical balance, add 500ml of water, stir and dissolve with a glass rod. Weigh 8.4g of sodium bicarbonate powder with an analytical balance, and add 500ml of medical physiological water for injection. Stir with a glass rod to dissolve. Then add 10-30ml 0.1mol / L Na 2 CO 3 solution to adjust its pH to about 8.8.

[0017] Solution: 3mol / L sodium acetate solution (pH5.5)

[0018] Weigh 4.0 g of sodium acetate trihydrate powder with an analytical balance, and then add 8 ml of distilled water. Stir with a glass rod to dissolve. Then add 1ml of glacial acetic acid. Mix well. Check the pH with pH paper. The pH was adjusted to 5.5 by adding glacial acetic acid drop by drop. Finally add water to make up to 10ml.

[0019] , Digoxigenin probe labeling oper...

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Abstract

The invention provides a method for labeling in situ hybridization centromere probes with digoxin, and belongs to the technical field of nucleic acid labeling in molecular biology. The method of using digoxigenin to label in situ hybridization centromere probes is mainly used to label digoxigenin on centromere probes, and then the centromere probes can be applied to FISH probes to detect centromere purpose of change. The method of using digoxigenin to label in situ hybridization centromere probes provided by the invention can effectively label digoxigenin molecules on oligonucleotides, and can be applied to the research of molecular markers and clinical molecular diagnosis.

Description

technical field [0001] The present invention relates to the field of nucleic acid labeling in molecular biology, in particular to the labeling of oligonucleotide probes, where digoxigenin molecules are labeled on oligonucleotide centromere probes, and then used in in situ hybridization to detect centromere application. Background technique [0002] In the field of molecular biology, nucleic acid probe labeling has been discovered in the 1970s, but when it comes to the internal work of laboratories or reagent research and development, the technology is still immature, there are many links, and it often fails. The main reason is that there are many steps, and the reagents used are many and complicated. From the development of radioactive labels, to the widespread use of non-radioactive techniques today. The need for probe labeling is increasing. Therefore, finding a convenient and rapid probe labeling technology has become an urgent problem to be solved. The genome is crac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6806
CPCC12Q1/6806C12Q2563/179
Inventor 不公告发明人
Owner GUANGZHOU EXONS BIOLOGICAL TECH
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