Detection method and application of fingerprint spectrum of Zhibai Dihuang pill

A technology of Zhibai Dihuang Pill and fingerprint spectrum, applied in the field of traditional Chinese medicine component detection, can solve the problem of lack of qualitative and quantitative research of Zhibai Dihuang Pill and so on.

Pending Publication Date: 2021-01-29
HEHUANG PHARMA SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is a lack of systematic qualitative and quantitative research on Zhibai Dihuang Pills

Method used

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  • Detection method and application of fingerprint spectrum of Zhibai Dihuang pill
  • Detection method and application of fingerprint spectrum of Zhibai Dihuang pill
  • Detection method and application of fingerprint spectrum of Zhibai Dihuang pill

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Experimental program
Comparison scheme
Effect test

preparation example Construction

[0102] Preparation of the test solution: crush the crude drug of Zhibai Dihuang Pills and pass through a No. 4 sieve to obtain the crude drug powder of Bai Dihuang Pills, and carry out at a dose of 3-10KGy 60 Co-γ-ray irradiation, 0 The maximum absorbed dose of Co-γ-ray irradiation is ≤6KGy. Take 1.0g of crude drug powder of Baidihuang Pills, put it in a stoppered conical flask, precisely add 15-25ml of methanol aqueous solution with a concentration of 50-100% by volume, weigh it, seal it with an ultrasonic instrument at a power of 80- Ultrasonic extraction at 120W and frequency of 30-50kHz for 15-60min, cooling and reweighing, make up the lost weight with 50-100% methanol aqueous solution, take the supernatant, and filter it with a microporous membrane with a pore size of 0.45μm , Take the continued filtrate to obtain the test solution.

[0103] Preparation of reference substance solution: Accurately weigh morroniside, loganin, mangiferin, berberine hydrochloride and paeono...

Embodiment 1

[0133] 1. Sample pretreatment

[0134] Preparation of the test solution: crush the crude drug of Zhibai Dihuang Pills of 200301 batches and pass through a No. 4 sieve to obtain the crude drug powder of Bai Dihuang Pills, which is carried out at a dose of 6KGy. 60 Co-γ-ray irradiation, 0 The maximum absorbed dose of Co-γ-ray irradiation is 6KGy. Take 1.0 g of crude drug powder of Baidihuang Wan, put it in a conical flask with a stopper, add 20ml of methanol aqueous solution with a volume percentage concentration of 80% precisely, weigh it, and seal it with an ultrasonic instrument at a power of 100W and a frequency of 40kHz. Ultrasonic extraction for 30 minutes, cooled, weighed again, supplemented the lost weight with 80% methanol aqueous solution, took the supernatant, filtered it with a microporous membrane with a pore size of 0.45 μm, and took the subsequent filtrate to obtain the test solution.

[0135] Preparation of reference substance solution: Accurately weigh morroni...

Embodiment 2

[0149] Adopt the detection method of Zhibai Dihuang Pills fingerprint spectrum set up in above-mentioned embodiment 1 to detect many batches of Zhibai Dihuang Pills, obtain the fingerprint spectrum of need testing solution and the fingerprint spectrum of reference substance solution, the obtained need testing substance The fingerprint data was imported into the "Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System" 2012 version software issued by the National Pharmacopoeia Committee, and compared with the standard fingerprint of Zhibai Dihuang Pills obtained under the same fingerprint detection conditions, the Zhibai Dihuang The similarity between the fingerprints of the pills and the standard fingerprints of Zhibai Dihuang pills ≥ 0.991 is taken as the standard, and the median method is used for the generation of the comparison spectra, and the time window width is 0.2. image 3 . The standard fingerprints of Zhibai Dihuang Pills were calibrated, includin...

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Abstract

The invention provides a detection method of a fingerprint spectrum of a Zhibai Dihuang pill. The invention further provides an application of the fingerprint spectrum detection method of the Zhibai Dihuang pill in quality detection of components in the Zhibai Dihuang pill and a quality detection method of the Zhibai Dihuang pill. The invention further provides a method for determining the contentof the five components in the Zhibai Dihuang pill. The invention further provides a screening method of the fingerprint spectrums of the multiple medicinal materials in the Zhibai Dihuang pill. According to the detection method and application of the fingerprint spectrum of the Zhibai Dihuang pill, the high performance liquid fingerprint spectrum of the Zhibai Dihuang pill is established, five chemical components in the Zhibai Dihuang pill can be quantitatively analyzed, affiliation confirmation is carried out on chromatographic peaks of single medicinal materials in the Zhibai Dihuang pill,effective monitoring can be carried out from a raw material source, the quality of raw medicinal materials is strictly controlled, and the amount of effective components in the Zhibai Dihuang pill isensured to be relatively stable, so that the safety and effectiveness of clinical medication are ensured.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine component detection, and relates to a fingerprint detection method of Zhibai Dihuang Pills and its application, in particular to a fingerprint detection method of Zhibai Dihuang Pills and its application in quality detection. Background technique [0002] Zhibai Dihuang Wan is a variety included in the 2020 edition of "Chinese Pharmacopoeia". It is made of fine powder of 8 traditional Chinese medicinal materials such as Anemarrhena, Cortex Phellodendron, Rehmannia glutinosa, Chinese yam, Cornus officinalis, Poria cocos, Moutan bark and Alisma, sieved, mixed, and dried with refined honey and watered into pills. Black water honey pill. It has the effect of nourishing yin and reducing fire. It is mainly used clinically for symptoms such as yin deficiency and hyperactivity of fire, hot flashes and night sweats, dry mouth and sore throat, tinnitus and spermatorrhea, and short red ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8809
Inventor 田禾苗温方方姜鹏詹常森张正光
Owner HEHUANG PHARMA SHANGHAI
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