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Prostate cancer early diagnosis method

A technology for early diagnosis and prostate cancer, applied in the field of clinical diagnosis, can solve the problems of non-specificity of prostate tissue, limited prospects, not a secreted protein, etc.

Pending Publication Date: 2021-01-29
海军军医大学第一附属医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] AMACR can effectively distinguish prostate cancer from benign disease in tissues, but is not a secreted protein and is not prostate tissue specific, which greatly limits its development as a blood marker for prostate cancer

Method used

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  • Prostate cancer early diagnosis method
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Experimental program
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Embodiment

[0014] Example: Using PSMA-positive tumor cells in circulating blood and AMACR expression levels in extracellular microvesicles to identify prostate cancer and benign prostatic hyperplasia.

[0015] Experimental method: add an appropriate amount of biotin-labeled PSMA antibody and appropriate amount of streptavidin-labeled magnetic beads into a 1.5ml EP tube and mix well, and place the EP tube on a rotary mixer overnight; the next day, put the EP tube in On the magnetic stand, wash the magnetic beads 3 times with PBS, and obtain the magnetic beads coated with PSMA antibody for later use. Take 2ml of peripheral venous blood from the patient, let it stand at room temperature for 5 minutes, centrifuge at 4000g for 15 minutes, and take the supernatant for use. Take 200ul of serum in a 1.5ml EP tube, add PBS to dilute to 1ml, add an appropriate amount of PSMA antibody-coated magnetic beads, place the EP tube on a rotary mixer overnight; put the EP tube on a magnetic stand the next ...

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Abstract

The invention relates to an early diagnosis method for prostate cancer. The early diagnosis method is characterized in that the prostate cancer is early diagnosed by using the expression quantity of AMACR (alpha methyl acyl coenzyme A racemase) in tumor cells with positive PSMA (prostate specific membrane antigen) in body fluid and extracellular microvesicles.

Description

technical field [0001] The present invention relates to the field of clinical diagnosis, in particular to an early diagnosis based on the expression of AMACR (α-methylacyl-CoA racemase) in PSMA (prostate-specific membrane antigen)-positive tumor cells in body fluids and extracellular microvesicles Prostate Cancer Approach. Background technique [0002] Prostate cancer is one of the most common malignant tumors in men, and its incidence increases with age. It ranks third in the incidence of male malignant tumors worldwide and sixth in its mortality rate. Compared with developed countries in Europe and the United States, the incidence of prostate cancer in my country is relatively low, but with the continuous development and progress of society, the incidence has continued to rise rapidly in recent years, and it has become one of the important diseases affecting the health of Chinese men. [0003] At present, PSA detection is still the main method for early screening of prost...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/543G01N33/68
CPCG01N33/54326G01N33/57434G01N33/6803G01N33/6848
Inventor 曹智王富博吉进徐欢杨悦许亚龙陈希何静怡孙颖浩
Owner 海军军医大学第一附属医院