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Staphylococcus nepalensis resistant to high-salt environment and its culture method

A staphylococcus and culture method technology, applied in the directions of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., to achieve the effects of reducing fermentation costs, enriching nutrients, and excellent resistance to high-salt environments

Active Publication Date: 2022-02-18
JINAN HANGCHEN BIOTECHNOLOGY CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] There are few publications about the culture method of Staphylococcus nepalis strains and its ability to act on wastewater, especially high-salt fermentation wastewater, and to effectively treat wastewater
[0003] As for the high-salt fermentation waste water, CN109136095A discloses a kind of monosodium glutamate fermentation waste water, adopts three kinds of microorganisms, can effectively utilize waste water to prepare microbial cell protein, purify waste water simultaneously, but whether these three kinds of microorganisms have high salt tolerance Performance and whether it has a good ability to purify wastewater in a high-salt environment, the above-mentioned patents do not involve

Method used

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  • Staphylococcus nepalensis resistant to high-salt environment and its culture method

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Experimental program
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Effect test

Embodiment 1

[0036] The salt-tolerant bacterium Staphylococcus nepalensis (Staphylococcus nepalensis) A-A10-1 was preserved in the China Center for Type Culture Collection on September 16, 2020, with the preservation number: CCTCC NO: M 2020505; preservation address: Wuhan University, Wuhan, China. Its characteristics are as follows:

[0037] After the strain was cultured in LB medium at 37°C for 18 hours, the culture medium showed a light yellow opaque single colony with a moist and shiny surface, smooth edges, and a diameter of 3-4mm; Gram staining was positive for G+, and the cells were round or round. Oval, often grape-like arrangement, no flagella, no capsule, no spores.

[0038] The 16sRNA sequence list of the aforementioned Staphylococcus nepalensis (Staphylococcus nepalensis) A-A10-1 is shown in Sequence 1.

[0039] Above-mentioned Staphylococcus nepalensis (Staphylococcus nepalensis) A-A10-1, its shaking flask culture method is as follows:

[0040] The seed medium of Staphylococ...

Embodiment 2

[0044] The seed culture medium of Staphylococcus nepalensis (Staphylococcus nepalensis) A-A10-1 is composed of the following raw materials in weight percentage: 0.35% of yeast powder, 0.75% of peptone, and 0.75% of sodium chloride;

[0045] The shake flask fermentation medium of Staphylococcus nepalensis (Staphylococcus nepalensis) A-A10-1 is composed of the following raw materials in weight percentage: high-concentration glutamic acid waste liquid containing 14.56wt% ammonium sulfate, and the pH value is adjusted to 7.2 by using ammonia water;

[0046]Staphylococcus nepalensis (Staphylococcus nepalensis) A-A10-1 activation culture method is: the single bacterium colony on the LB plate is inoculated in the seed culture medium, at temperature 40 ℃, 180rpm shaking table shaking culture 16h, after that according to 5% The inoculation amount was inserted into the fermentation medium, and the temperature was 37° C., 180 rpm shaker cultured for 72 hours, the residual sugar was reduce...

Embodiment 3

[0048] The seed medium of Staphylococcus nepalensis (Staphylococcus nepalensis) A-A10-1 is composed of the following raw materials in weight percentage: yeast powder 0.45%, peptone 1.2%, sodium chloride 0.65%;

[0049] The shake flask fermentation medium of Staphylococcus nepalensis (Staphylococcus nepalensis) A-A10-1 is composed of the following raw materials in weight percentage: high-concentration glutamic acid waste liquid containing 10.23wt% ammonium sulfate, and the pH value is adjusted to 5.0 by using ammonia water;

[0050] Staphylococcus nepalensis (Staphylococcus nepalensis) A-A10-1 activation culture method is: the single bacterium colony on the LB plate is inoculated in the seed culture medium, at temperature 40 ℃, 180rpm shaking table shakes and cultivates 18h, and then according to 4% The inoculation amount was inserted into the fermentation medium, and the temperature was 40° C., and the shaker was cultured at 180 rpm for 82 hours. The residual sugar was reduced ...

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Abstract

The invention belongs to the technical field of microorganism culture, and in particular relates to a method for cultivating staphylococcus nepalis capable of enduring a high-salt environment. The Staphylococcus Nepalis tolerant to a high-salt environment is characterized in that the Staphylococcus Nepalis was preserved in the China Center for Type Culture Collection on September 16, 2020, and the preservation number is CCTCC NO: M 2020505. The method for cultivating Staphylococcus Nepali provided by the present invention comprises the following steps: S1: preparing a culture medium for Staphylococcus Nepali; S2: preparing a fermentation medium for Staphylococcus Nepali; S3: fermenting and cultivating. The beneficial effects of the present invention are: the Nepalese staphylococcus provided by the present invention has excellent high-salt resistance, can adapt to the treatment of wastewater in a high-salt environment, and provides a new microbial treatment method for the treatment of high-salt fermentation wastewater; The invented strain has excellent effect in treating high-salt wastewater; it can reduce the residual sugar content in fermentation wastewater to 0.3-0.8%.

Description

technical field [0001] The invention belongs to the technical field of microorganism culture, and in particular relates to a staphylococcus nepalese tolerant to a high-salt environment and a culture method of the strain. Background technique [0002] There are few literatures about the cultivation method of the Staphylococcus nepallius strain and its ability to act on wastewater, especially high-salt fermentation wastewater, and to effectively treat wastewater. [0003] As for the high-salt fermentation waste water, CN109136095A discloses a kind of monosodium glutamate fermentation waste water, adopts three kinds of microorganisms, can effectively utilize waste water to prepare microbial cell protein, purify waste water simultaneously, but whether these three kinds of microorganisms have high salt tolerance Performance and whether it also has a good ability to purify wastewater in a high-salt environment, the above-mentioned patents do not involve. In fact, in organic acid ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/44
CPCC12N1/20C02F3/34C12R2001/44C12N1/205
Inventor 赵晨李昆仑赵林李宝君岳秋林苏乐
Owner JINAN HANGCHEN BIOTECHNOLOGY CO LTD
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