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Hydrolysate enriched with biological bioactive peptide for accelerating beer yeast fermenting and its preparation method and application

A bioactive peptide and brewer's yeast technology, which is applied in the field of hydrolyzates rich in bioactive peptides that promote fermentation of brewer's yeast, can solve the problems of low benefit and bioavailability, difficulty in breaking walls, and high residual sugar content, and improve production efficiency. and economic benefits, shorten the fermentation time, and reduce the effect of residual sugar content

Inactive Publication Date: 2011-05-11
SOUTH CHINA UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the thick cell wall of Corynebacterium glutamicum, it is difficult to break the wall, and it is difficult to develop and utilize. At present, Corynebacterium glutamicum is mainly used in fertilizer production, and the benefit and bioutilization value are low.
[0003] The conventional beer fermentation process is relatively long, while the ethanol yield is low, and the residual sugar content in the fermentation liquid is high, which affects the production efficiency of the manufacturer and the quality of the product

Method used

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  • Hydrolysate enriched with biological bioactive peptide for accelerating beer yeast fermenting and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Mix 1 kg of Corynebacterium glutamicum and water in a 1:1 weight ratio, and stir evenly to prepare Corynebacterium glutamicum liquid;

[0024] (2) Adopt high-pressure homogenizer to carry out homogenization treatment to Corynebacterium glutamicum, 30MPa homogenization treatment 2 times;

[0025] (3) At 55°C, adjust the pH to 6.0 with sodium hydroxide solution, first use papain to hydrolyze, and use the pH-Stat method to control the degree of hydrolysis to 5.0%, then add chymotrypsin to hydrolyze, and use pH-Stat method to control the degree of hydrolysis to 10.2%, to obtain the enzymatic solution;

[0026] (4) Keep the enzymatic solution at 85°C for 18 minutes to inactivate the protease;

[0027] (5) Centrifuge the enzymatic solution at 6000r / m for 15min to remove the cell wall of Corynebacterium glutamicum;

[0028] (6) Pass the hydrolyzate through a 5000Da ultrafiltration membrane to remove large molecular weight polypeptides and proteins, and obtain an enzymati...

Embodiment 2

[0032] (1) each 1kg of Corynebacterium glutamicum and water are mixed, stirred evenly to prepare Corynebacterium glutamicum liquid;

[0033] (2) Adopting a high-pressure homogenizer to homogenize the Corynebacterium glutamicum liquid at 35MPa for 2 times;

[0034] (3) At 60°C, use sodium hydroxide solution to adjust the pH to 6.8, first use alkaline protease for hydrolysis, and when the degree of hydrolysis reaches 5.5% (pH-Stat method), add trypsin for hydrolysis, use pH-Stat method Control the degree of hydrolysis to 11.2% to obtain the enzymatic solution;

[0035] (4) Keep the enzymatic hydrolysis solution of (3) at 88°C for 15 minutes to inactivate the protease;

[0036] (5) Centrifuge the enzymatic solution at 6000r / m for 15min to remove the cell wall of Corynebacterium glutamicum;

[0037] (6) Pass the hydrolyzate through a 5000Da ultrafiltration membrane to remove large molecular weight polypeptides and proteins, and obtain a hydrolyzate rich in bioactive peptides tha...

Embodiment 3

[0039] (1) Mix Corynebacterium glutamicum and water in a mass ratio of 1:1 to prepare Corynebacterium glutamicum liquid;

[0040] (2) Adopt high-pressure homogenizer to carry out homogenization treatment to Corynebacterium glutamicum liquid, under the condition of 45MPa homogenization treatment 2 times;

[0041] (3) At 65°C, use sodium hydroxide solution to adjust the pH to 7.5, first use papain to hydrolyze, use the pH-Stat method to control the degree of hydrolysis to 5.8%, add trypsin for hydrolysis, and use the pH-Stat method to control the hydrolysis to 11.8%;

[0042] (4) Keep the enzymatic hydrolysis solution of (3) at 90°C for 15 minutes to inactivate the protease;

[0043] (5) The hydrolyzate was centrifuged at 6000r / m for 15min to remove the cell wall of Corynebacterium glutamicum;

[0044] (6) Pass the hydrolyzate through a 5000Da ultrafiltration membrane to remove large molecular weight polypeptides and proteins, and obtain a hydrolyzate rich in bioactive peptide...

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Abstract

The present invention discloses one kind of hydrolysate containing rich bioactive peptides capable of promoting beer yeast fermentation. The hydrolysate is prepared with the glutamic acid corynebacteria, and the bioactive peptides have molecular weight of 500-2000 Da and account for 50-60 wt% of the total nitrogen. Using the hydrolysate in beer production can shorten the fermentation period, raise ethanol content in the fermented liquid and lower the residual saccharide content in the fermented liquid obviously.

Description

technical field [0001] The invention relates to the field of food biotechnology, in particular to a hydrolyzate rich in biologically active peptides for promoting beer yeast fermentation, its preparation method and application. Background technique [0002] Corynebacterium glutamicum, a Gram-positive bacterium, is strictly aerobic and does not produce spores. It is the main component of the waste obtained by ultrafiltration after glutamic acid fermentation. Corynebacterium glutamicum is rich in protein, nucleic acid, carbohydrate, ash, vitamins and trace elements necessary for microbial growth, and its protein content is more than 70% on a dry basis. The amino acid composition of Corynebacterium glutamicum protein has obvious characteristics, among which glutamic acid accounts for 30.60% of the total protein, aspartic acid accounts for 8.47%, alanine accounts for 7.45%, glycine accounts for 3.92%, and a total of 50.44%. , these four amino acids are organic nitrogen sources ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12C11/00C12P21/02C12R1/15
Inventor 赵谋明黄立新崔春徐正康
Owner SOUTH CHINA UNIV OF TECH
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