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Enzyme-containing composition and method for improving the stability of liquid bacterial agents

A technology of liquid bacterial agent and enzyme composition, applied in the field of agricultural biology, can solve the problems of poor application effect, decreased product quality, increased microbial mortality, etc., and achieves improved storage stability, extended shelf life, and reduced microbial mortality. Effect

Active Publication Date: 2018-05-08
SHANDONG ZOETICLAND BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that the conventional amount of preservatives is not enough to completely inhibit the metabolism of microorganisms, and the application effect is not good. High concentrations of preservatives will lead to increased mortality of microorganisms and a decline in product quality.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: the preparation of enzyme composition

[0017] The preparation method of the enzyme-containing composition of the present invention comprises the following steps:

[0018] 1) Preparation of enzyme preparations: the enzyme preparations used to prepare the enzyme-containing composition were purchased from Taian Xindeli Biotechnology Co., Ltd. except for sucrase. The enzyme activity of glucose oxidase was 100,000 U / g, and the implemented product standard It is DB13 / T1444-2011 "Feed Additive Glucose Oxidase"; the enzyme activity of catalase is 10000U / g, and the product standard implemented is QB / T 4614-2013 "Industrial Catalase Preparation"; glucoamylase The enzyme activity is 50000U / g, and the product standard implemented is GB 8276-2006 "Food Additive Glucoamylase Preparation". Sucrase was purchased from Novozymes (China) Biotechnology Co., Ltd., and the product specification was 20000U / g.

[0019] 2) Preparation of other raw materials: trehalose is produc...

Embodiment 2

[0022] Embodiment 2: the application in the production of Bacillus subtilis liquid bacterial agent.

[0023] 1) Activation of strains: transfer Bacillus subtilis into the slant of NA medium test tube, and incubate at 35° C. for 24 hours for activation. The formula of the NA medium is: beef extract 3g, peptone 10g, NaCl 5g, agar 20g, water 1000ml, pH 7.0.

[0024] 2) Triangular flask seed preparation: Scrape activated Bacillus subtilis with an inoculation loop, inoculate in NB medium, and culture at 35° C. for 24 hours. The NB medium is nutrient broth medium, and its formula is 3g of beef extract, 10g of peptone, 5g of NaCl, 1000ml of water, and pH 7.0.

[0025] 3) Preparation of seed tank strains: Transfer the seeds of the triangular flask to a 10L seed tank equipped with 6L NB medium with an inoculation amount of 2%, and cultivate them at 35°C for 20h, with a stirring speed of 200rpm throughout the whole process, and a ventilation volume of 0-6h. 3L / min, 6-20h ventilation r...

Embodiment 3

[0032] Embodiment 3: the preparation of enzyme composition

[0033] Raw material preparation is with embodiment 1.

[0034] Mix the above raw materials uniformly according to the following parts by weight: 29 parts of glucose oxidase, 9 parts of catalase, 19 parts of glucoamylase, 9 parts of sucrase, 12 parts of trehalose, 6 parts of chitosan, 1 part of natamycin Parts, 10 parts of potassium sorbate, 5 parts of propyl gallate, totally 100 parts.

[0035]In the enzyme complex prepared according to the above ratio, each 1g contains the following ingredients: glucose oxidase 29000U, catalase 900U, glucoamylase 9500U, sucrase 1800U, trehalose 0.12g, chitosan 0.06g, natamycin Sodium 0.01g, potassium sorbate 0.10g, propyl gallate 0.05g.

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Abstract

The invention discloses an enzyme-containing composition and a method for improving the stability of a liquid microbial agent. The enzyme-containing composition contains the following components: glucose oxidase, catalase, glucoamylase, sucrase, trehalose, chitosan, natamycin, potassium sorbate and propyl gallate. The method for improving the stability of the liquid bacterial agent is as follows: after the fermentation of the bacillus-like microorganisms, add hydrochloric acid to the fermentation broth to acidify to pH 5.0-5.5; then add the above-mentioned enzyme-containing composition, and then keep the fermentation broth at 40-45°C About 6 hours; after cooling down to room temperature, add EDTA, mix well, and fill. The present invention can reduce the residual sugar content in the liquid inoculum produced by the liquid fermentation process using sugary raw materials as the carbon source, inhibit the metabolic activity of microorganisms, and at the same time have a protective effect on microbial cells, and can reduce the bulging of bottles during product storage ( Bag) rate and microbial mortality, improve storage stability, and extend shelf life.

Description

technical field [0001] The invention relates to an enzyme-containing composition and a method for improving the stability of a liquid microbial agent, belonging to the field of agricultural biotechnology. Background technique [0002] Microbial fertilizers refer to biological products containing active microorganisms, which can promote plant growth, reduce diseases, improve soil, and improve the quality of agricultural products. It is one of the new fertilizers that are prioritized by the country and has broad development prospects. There are two types of commercially available microbial fertilizers: solid and liquid. Among them, the liquid bacterial agent can be directly formulated from the fermentation broth, without solid-liquid separation and drying and other production links. The process is simple, the cost is low, and it is easy to be miscible with water and easy to use. However, compared with solid microbial agents, liquid microbial agents have poorer storage stabili...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/04C12R1/125C12R1/10C12R1/11C12R1/07
Inventor 辛寒晓孙中涛王维超王泽利范学明
Owner SHANDONG ZOETICLAND BIOLOGICAL TECH
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