Biocontrol strain YW-1 as well as preparation of biocontrol inoculant and application thereof

A technology of YW-1 and biocontrol strains, applied in the direction of biochemical equipment and methods, applications, biocides, etc., can solve the problems of limited control range, single mode of action of pathogens, and inability to adapt to the field environment, etc., to achieve good control Effect, overcoming single disease prevention and control, large practical application potential and application range

Active Publication Date: 2021-02-12
HUAIYIN TEACHERS COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the biggest obstacle hindering the development of biological control is the limited range of control and the instability of field biological

Method used

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  • Biocontrol strain YW-1 as well as preparation of biocontrol inoculant and application thereof
  • Biocontrol strain YW-1 as well as preparation of biocontrol inoculant and application thereof
  • Biocontrol strain YW-1 as well as preparation of biocontrol inoculant and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The invention relates to a biocontrol strain YW-1 for preventing and treating various plant soil-borne diseases, which was isolated from the soil of pepper greenhouses in Wudun Town, Huai'an City, Jiangsu Province in 2015, and is Myroides odoratimimus. On September 9, 2020, it was deposited in the General Microbiology Center of China Committee for Culture Collection of Microorganisms (Address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Chinese Academy of Microbial Sciences), and the culture preservation number is CGMCC NO.20620 .

[0025] 1. Isolation and purification of biocontrol strain YW-1

[0026] (1) First use a shovel to dig out the soil at the base of the plant to a depth of 10 cm, then use a small brush to collect the soil attached to the surface of the plant root, put it into a small ziplock bag, mark the number and quickly bring it back to the laboratory for further processing.

[0027] (2) In this study, LB medium was use...

Embodiment 2

[0047] Preparation of YW-1 Biocontrol Agent

[0048] The biocontrol strain YW-1 was cultured in LB culture medium (tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, pH=7.2) at 28°C and 180rpm for 12-16h to obtain the total concentration of viable bacteria 1×109-1×1010CFU / mL bacterial suspension, then centrifuge the bacterial suspension at 6000rpm for 10min to collect the bacterial cells, dilute with sterilized water to make a concentration of 10 7 CFU / mL biocontrol agents.

Embodiment 3

[0050] Verification of the effect of YW-1 biocontrol fungicide on various plant soil-borne diseases in greenhouse pots

[0051] 1. Greenhouse pot test to detect the control effect of YW-1 biocontrol fungicide on tomato and pepper bacterial wilt

[0052] Cultivate tomato seedlings and pepper seedlings using plug trays, transplant when they grow to 3-4 true leaves, and use 20mL 10 7 CFU / mL bacterial agent was used to irrigate the roots, and the control group was treated with clean water. One week after transplanting, 10 7 CFU / mL Ralstonia solanacearum 3721 bacterial suspension 20mL. 24 strains in each treatment group, repeated three times. Under the conditions of temperature 25-28°C, relative humidity 60%, and light 12h / 12h, the disease progression was investigated after 4 weeks of cultivation, and the disease severity and control effect were calculated.

[0053] According to the disease classification standard proposed by Kempe and Sequeria in 1983, the calculation formula o...

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Abstract

The invention discloses a biocontrol strain YW-1 as well as preparation of a biocontrol inoculant and an application thereof, and belongs to the technical field of plant protection. The bacterial strain is Myroides odoratimimus, and the preservation number of the bacterial strain is CGMCC NO. 20620; the biocontrol strain YW-1 is subjected to shaking culture in an LB culture solution for 12-16 hours at 30 DEG C and 180 rpm to enable the total concentration of viable bacteria in a bacterial suspension to be 1*10<9>-1*10<10> CFU/mL, then the bacterial suspension is centrifuged for 10 min at 6000rpm to collect thalli, and the thalli are diluted with sterilized water to prepare the biocontrol microbial agent with the concentration of 10<7> CFU/mL. When the host plants are transplanted, the fungus is used to irrigate the roots of 20mL/plant, which can effectively preventvarious soil-borne diseasessuch asmelon wilt, tomato and pepper bacterial wilt and pepper phytophthora blight, compared with a blank control group, the prevention and treatment effects can reach 50% or above.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and in particular relates to the preparation and application of a biocontrol strain YW-1 and a biocontrol agent. Background technique [0002] Facility agriculture is a modern agricultural production mode that comprehensively applies engineering equipment technology, biotechnology and environmental technology to create a suitable environment for the growth and development of animals and plants, and realize the production of animals and plants. The development of facility vegetables not only alleviates the contradiction between production season and consumption balance, but also can use facility cultivation to produce high-grade vegetables, famous and high-quality vegetables, and seasonal vegetables, and increase the variety of vegetables to meet consumption at different levels of society. need. my country has become the country with the largest facility area in the world, and it is stil...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/20A01P3/00A01P1/00C12R1/01
CPCC12N1/20A01N63/20C12R2001/01C12N1/205
Inventor 杨威闫海霞董光辉邱雨生朱跃龙张孜郭为李师默张磊罗玉明
Owner HUAIYIN TEACHERS COLLEGE
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