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A kind of industrialized fermentation production method of raspberry ketone

A production method and technology of raspberry ketone, which are applied in the field of industrialized fermentation production of raspberry ketone, can solve the problems of staying, unable to realize the industrial scale production of raspberry ketone, etc., and achieve the effect of an efficient production process

Active Publication Date: 2022-02-18
XIAMEN OMIC BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] In summary, the above methods are still at the laboratory level, and the industrial scale production of raspberry ketone cannot be realized yet.

Method used

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  • A kind of industrialized fermentation production method of raspberry ketone
  • A kind of industrialized fermentation production method of raspberry ketone
  • A kind of industrialized fermentation production method of raspberry ketone

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Embodiment 1

[0052]The microorganisms involved in this example were isolated and screened from numerous microorganisms in marine soil, among which: Actinomycetes sp. : CCTCC NO: M 2020584; Bacillus sp.OMK-75 was deposited in the China Center for Type Culture Collection on October 16, 2020, and the preservation number is: CCTCC NO: M 2020585; Saccharomycessp.OMK-76 was deposited in 2020 It was deposited in the China Center for Type Culture Collection on October 16, 2010, with the deposit number: CCTCC NO: M2020586.

[0053] Such as figure 1 Shown, a kind of industrialized fermentation production method of raspberry ketone comprises the steps:

[0054] (1) Using natural p-coumaric acid as raw material, p-hydroxybenzaldehyde is produced under the metabolism of actinomycetes sp.OMK-74, specifically including:

[0055] A. Take a tube of Actinomycetes sp.OMK-74 refrigerated in glycerin in a -80°C refrigerator and streak on a fresh, sterile plate, and place the obtained plate in an incubator at...

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Abstract

The invention discloses an industrial fermentation production method of raspberry ketone, comprising the following steps: (1) using natural p-coumaric acid as a raw material to generate p-hydroxybenzaldehyde under the metabolism of actinomycetes sp.OMK-74; 2) with the p-hydroxybenzaldehyde and acetone gained in step (1) as raw materials, generate 4-hydroxybenzylidene acetone under the metabolism of Bacillus sp.OMK-75; Benzylidene acetone is used as a raw material, and the raspberry ketone is reduced under the metabolism of yeast Saccharomyces sp.OMK-76. The invention realizes the high-efficiency biosynthetic production process of raspberry ketone through three-step fermentation.

Description

technical field [0001] The invention belongs to the technical field of raspberry ketone production, and in particular relates to an industrial fermentation production method of raspberry ketone. Background technique [0002] Raspberry ketone, also known as rubus ketone, has a chemical name of 4-p-hydroxyphenyl-2-butanone. Raspberry ketone is the main aroma component of raspberry. It is an elegant and fruity spice widely used at home and abroad. It is widely used in cosmetics and food, and can also be used in synthetic medicine. [0003] In the prior art, the report about the synthetic method of raspberry ketone is specifically as follows: [0004] (1) In 1996, Claudio Fuganti etc. used baker's yeast (Tetrahedron, 1996, 52.4041-4052) and Beauveria bassiana (Tetrahedron: Asymmetry, 1996, 7, 3129-3134) respectively to 4-hydroxybenzylidene acetone (such as figure 1 Shown) the ethylenic bond in the reduction preparation raspberry ketone. And published an article in 1998 (Journ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/26C12N1/20C12N1/16C12R1/04C12R1/07C12R1/85
CPCC12P7/26C12N1/20C12N1/16C12R2001/01C12R2001/07C12R2001/85
Inventor 邢晨光赵希景苗杨利洪英刘伟
Owner XIAMEN OMIC BIOTECH CO LTD
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