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36 results about "Saccharomyces sp." patented technology

Cytosolic isobutanol pathway localization for the production of isobutanol

ActiveUS20110076733A1Increase cytosolic localizationIncrease cytosolic activityFungiBacteriaYeastIsobutanol
The present invention provides recombinant microorganisms comprising isobutanol producing metabolic pathway with at least one isobutanol pathway enzyme localized in the cytosol, wherein said recombinant microorganism is selected to produce isobutanol from a carbon source. Methods of using said recombinant microorganisms to produce isobutanol are also provided. In various aspects of the invention, the recombinant microorganisms may comprise a cytosolically active isobutanol pathway enzymes. In some embodiments, the invention provides mutated, modified, and / or chimeric isobutanol pathway enzymes with cytosolic activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
Owner:GEVO INC

Microbial formulation and method of using the same promote plant growth

A plant growth promoting composition includes beneficial microbes and microbial activators. The beneficial microbes may include Bacillus spp., Azotobacter spp., Trichoderma spp., Saccharomyches spp., or combinations of these, the microtrial activators include enzymes, metabolites, carbohydrate yeast, humic material, staich, amino acids and micornutrients. The plant growth promoting composition may also include organic and inorganic fertilizers.
Owner:ULTRA BIOTECH

Biological deodorant

InactiveCN102732452AImprove the farmImprove the surrounding environmentFungiBacteriaRhodobacter sp.Biotechnology
The present invention discloses a biological deodorant. The biological deodorant is characterized in that the biological deodorant comprises molasses, water and a composite strain, wherein the composite strain comprises Bacillus sp, Actinomyces sp, Saccharomyces sp, Lactobacillus sp and Rhodobacter sp, a weight ratio of the bacteria is 2:2:1:3:2, the bacteria are inoculated into a medium prepared by dissolving molasses in water, and the sugar degree of the molasses is more than or equal to 5%. Compared to the biological deodorant in the prior art, the biological deodorant of the present invention has the following advantages that: various foul gases generated by a large amount of livestock and poultry manures due to breeding can be completely removed so as to improve environmental conditions of farms and farmers, reduce surrounding environmental pollution, and ensure lives and productions of local residents; a good foundation is established for comprehensive recovery and utilization, and harmless treatment of livestock and poultry manures, and strong technical supports are provided for improvement of circulation of agricultural economy.
Owner:绍兴虞林生物科技有限公司

Additive-free cigarette production process using flavored composite microbial agent

The invention discloses an additive-free cigarette production process using a flavored composite microbial agent. In the traditional cigarette production process, the flavored composite microbial agent is sprayed to incised tobacco shreds / leaf groups or surface of baked tobacco shreds / leaf groups, the tobacco shreds / leaf groups are fermented for 24-60 h at constant temperature of 25-45 DEG C and constant humidity of 30%-90%, the fermented tobacco shreds / leaf groups are dried under the condition of 25-180 DEG C, and the cigarette finished product is obtained when the moisture of the tobacco shreds / leaf groups reaches 10-15%. The composite microbial agent used in the process comprises bacillus sp. LBT1.0007, bacillus sp. LBT1.0013 and Saccharomyces sp. LBT1.0038. The bacterial stains contained in the composite microbial agent are separated and screen from alcoholized tobacco surfaces. The composite microbial agent is a new cigarette leaf group flavored composite microbial agent. The cigarette product produced by using the process has the advantages of no additive, good flavoring effect and the like.
Owner:NORTHWEST A & F UNIV +1

Lactic acid-tolerant ester-producing pichia pastoris

The invention discloses a lactic acid-tolerant ester-producing pichia pastoris, and belongs to the technical field of a bioengineering technology and a brewing biotechnology. The strain is preserved at the General Microbiology Center of the China Committee for Culture Collection of Microorganisms on April 24, 2017, the classification is named pichia kudriavzevii (Pichia kudriavzevii) and the preservation number is CGMCC No.14068. The pichia pastoris is high in environmental tolerance, is capable of tolerating 0-15% lactic acid, is capable of metabolizing to generate multiple ethyl ester volatile flavor substances, such as ethyl acetate, ethyl propionate, ethyl caprylate, phenethyl acetate and ethyl phenylacetate in the environment of 0-12% lactic acid, and is a brewing functional strain with excellent performance.
Owner:KWEICHOW MOUTAI COMPANY

Reduced by-product accumulation for improved production of isobutanol

ActiveUS20110201073A1Catalytic efficiency be enhanceEnhance abilityFungiBiofuels2-HydroxybutyrateYeast
The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and / or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
Owner:GEVO INC

Yeast and application thereof

The invention provides yeast Saccharomyces sp.ZB118. The yeast has the preservation number of GDMCC No: 60280. The yeast Saccharomyces sp.ZB118 provided by the invention has a very good aroma increasing effect, can be used for brewing fermented condiments including soy sauce and the like and can be used for generating a lot of small molecular substances including alcohols, esters and the like, andcharacteristic phenols aromatic substances including 4-vinyl-2-methoxyphenol and the like; an obtained product has a rich sauce flavor and the flavor of the product is remarkably improved.
Owner:FOSHAN HAITIAN GAOMING FLAVORING & FOOD +2

Composite microbial agent capable of degrading kitchen waste at normal temperature, its preparation method and its application

The invention discloses a composite microbial agent capable of degrading kitchen waste at normal temperature, its preparation method and its application, the composite microbial agent is composed of composite thalline and carriers. The composite thalline is composed of lactobacillus acidophilus zsq11 and microzyme Saccharomyces sp. zsp13; the carriers are composed of crushed peanut shells, sands, wood chips and wheat brans. The composite thalline accounts for 5-10% of composite microbial agent by weight percentage. According to the invention, high efficiency complex bacterium and carrier materials with good permeability are mixed to prepare the composite microbial agent, the composite microbial agent is placed in the kitchen waste for treating and acquires good operation effect at normal temperature, the degradation rate of the kitchen waste can reach more than 75%, so that high temperature required in the prior art can be solved. The microbiological treatment processes such as moisture filtering and the like can be omitted. The invention has the advantages of simple processing step, stable effect and no environmental pollution.
Owner:广州舒国生物科技有限公司

Microbial agent for composting organic material

The present invention discloses a microbial agent for composting an organic material. The microbial agent is characterized by comprising molasses, water and a composite strain, wherein the composite strain comprises Bacillus sp, Actinomyces sp, Saccharomyces sp, Lactobacillus sp and Rhodobacter sp, a weight ratio of the bacteria is 3:1:1:3:2, the bacteria are inoculated into a medium prepared by dissolving molasses in water, and the sugar degree of the molasses is more than or equal to 5%. Compared to the microbial agent in the prior art, the microbial agent of the present invention has the following advantages that: hundreds of millions of tons of straws, livestock and poultry manures and urban domestic waste are prepared into organic fertilizers each year, such that the pollution problem of hundreds of millions of tons of the waste each year is fundamentally solved, chemical fertilizer application in agriculture is reduced, energy consumption and pollution caused by chemical fertilizer are reduced, and the serious environmental pollution problem is substantially released.
Owner:绍兴虞林生物科技有限公司

Method for the production of heterologous polypeptides in transformed yeast cells

The invention describes industrial fermentation of a Saccharomyces yeast species for production of a heterologous product encoded by a plasmid or DNA contained in said Saccharomyces yeast species which method utilizes the substrate more efficiently and without fermentative metabolism resulting in formation of ethanol and other unwanted primary products of fermentative activity whereby high yields of the heterologous product are obtained. The Saccharomyces yeast species is preferably a Crabtree negative Saccharomyces species in particular Saccharomyces kluyveri.
Owner:NOVO NORDISK AS

Water purification microbial agent for eutrophicated water body

The present invention discloses a water purification microbial agent for a eutrophicated water body. The microbial agent is characterized in that the microbial agent comprises molasses, water and a composite strain, wherein the composite strain comprises Bacillus sp, Actinomyces sp, Saccharomyces sp, Lactobacillus sp and Rhodobacter sp, a weight ratio of the bacteria is 5:1:1:2:1, the bacteria are inoculated into a medium prepared by dissolving molasses in water, and the sugar degree of the molasses is more than or equal to 5%. Compared to the microbial agent in the prior art, the microbial agent of the present invention has the following advantages that: feed utilization rate is improved so as to reduce feed use and save cost; water quality of the aquaculture body is significantly improved, the aquatic product quality is improved, and technical supports are provided for green and ecological development of aquaculture.
Owner:绍兴虞林生物科技有限公司

Method for producing biodiesel by efficiently utilizing corncob residues via Rhodococcus

The invention provides biodiesel, which has low sulfur content, does not contain aromatic compounds and is an environment-friendly renewable energy source. Rhodococcus has a very strong carbon source conversion capability and can be used for highly efficiently converting lignocellulose which is difficult to utilize in daily life into fatty acid; and the oil accumulation amount of Rhodococcusopacus PD630 can be up to 86% of the dry weight of cells when glucose is used as a carbon source and the Rhodococcusopacus PD630 is a high-quality oil-producing strain. The invention provides a method for producing the fatty acid by efficiently utilizing corncob residues via the Rhodococcusopacus PD630. Compared with common saccharomycetes, the Rhodococcus has strong bearing capability on polar conditions and high carbon source conversion capability; and a fermentation condition is simple and the method is an industrial production manner of the biodiesel, which has very great potential.
Owner:ZHENJIANG BIO INNOVA BIOTECH

Solid microbial inoculum for remediating Cd in farmland soil

A solid microbial inoculum for remediating Cd in farmland soil, comprising: curtobacterium luteum, cupriavidus necator, sphingomonas parapaucimobilis, cellulosimicrobium cellulans, azotobacter sp., saccharomyces sp., streptomyces sp. and pseudomonas sp. Acetic acid solubility of Cd, Cu and Zn in soil that is remediated by the solid microbial inoculum disclosed by the invention can be decreased significantly.
Owner:SHANGHAI JIAO TONG UNIV

Recombinant yeast for increasing yield of L-menthol

ActiveCN112175849AMetabolic synthesis is beneficialOvercoming Factors of Low Fermentation YieldFungiMicroorganism based processesSaccharomycesCulture mediums
The invention discloses recombinant yeast for increasing the yield of L-menthol, and belongs to the technical field of metabolic engineering. The yeast is used as a starting strain, an inducible promoter is adopted to express ERG20WW or / and NPPS genes, and an MVA metabolic synthesis path is reconstructed, so that compounds IPP and DMAPP tend to synthesize compounds GPP and NPP more, and metabolicsynthesis of the L-menthol is further facilitated. The inducible promoter is adopted for over-expression of an L3H gene, the expression quantity of an L3H enzyme is increased, the conversion of limonene into trans-isopiperitol is promoted, or tLIS and L3H genes are expressed through protease fusion, and the factor of the too low enzyme activity of the L3H enzyme causing the low fermentation yieldof the L-menthol is overcome. A YPD liquid culture medium is adopted for fermentation for 4 d, a 10% galactose aqueous solution is added in the later period from fermentation to logarithmic phase to induce expression of EEG20WW and NPPS genes and L-menthol metabolic pathway genes, normal growth and reproduction of cells are guaranteed, and the fermentation yield of the L-menthol reaches 30.14 mg / Lat most.
Owner:JIANGNAN UNIV

Heterologous Expression of Fungal Cellobiohydrolase 2 Genes in Yeast

ActiveUS20130217072A1Augment cellulose hydrolysisFacilitate ethanol productionAnimal cellsBacteriaHeterologousEnzyme Gene
The present invention provides for heterologous expression of polypeptides encoded by wild-type and codon-optimized cbh2 genes from the organisms Cochliobolus heterostrophus, Gibberella zeae, Irpex lacteus, Volvariella volvacea, and Piromyces sp. in host cells, such as the yeast Saccharomyces cerevisiae. The expression in such host cells of the corresponding genes, and variants and combinations thereof, result in improved specific activity of the expressed cellobiohydrolases. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.
Owner:STELLENBOSCH UNIVERSITY

Microbial starter for rice straw fermentation and preparation method thereof

InactiveCN109336654AGive full play to the purpose of treating waste with wasteFully utilizeOrganic fertilisersFertilizer mixturesBacillus licheniformisBacillus thuringiensis
The invention relates to the technical field of microbial fermentation, specifically to a microbial starter for rice straw fermentation and a preparation method of the microbial starter for the rice straw fermentation. The starter is prepared from the following raw materials: corn stalks, tomato stalks, Pseudomonas stutzeri, Bacillus thuringiensis, Bacillus mucilaginosus, Phanerochaete chrysosporium Burdsall, Bacillus licheniformis, Trichoderma viride, Trichoderma koningii, Trichoderma reesei, Saccharomyces sp, cellulase, protease and phosphorylase. The microbial starter for the rice straw fermentation and the preparation method of the microbial starter for the rice straw fermentation disclosed by the invention can solve the technical problem of difficult degradation of rice straw, the microbial starter disclosed by the invention has a rapid temperature rise during use, thereby being beneficial to prolonging the high-temperature fermentation time and improving the degradation rate of cellulose and hemicellulose in the rice straw, and fermented final products are rich in nutritional components.
Owner:HENAN UNIV OF SCI & TECH

Composite microbial agent capable of degrading kitchen waste at normal temperature, its preparation method and its application

The invention discloses a composite microbial agent capable of degrading kitchen waste at normal temperature, its preparation method and its application, the composite microbial agent is composed of composite thalline and carriers. The composite thalline is composed of lactobacillus acidophilus zsq11 and microzyme Saccharomyces sp. zsp13; the carriers are composed of crushed peanut shells, sands, wood chips and wheat brans. The composite thalline accounts for 5-10% of composite microbial agent by weight percentage. According to the invention, high efficiency complex bacterium and carrier materials with good permeability are mixed to prepare the composite microbial agent, the composite microbial agent is placed in the kitchen waste for treating and acquires good operation effect at normal temperature, the degradation rate of the kitchen waste can reach more than 75%, so that high temperature required in the prior art can be solved. The microbiological treatment processes such as moisture filtering and the like can be omitted. The invention has the advantages of simple processing step, stable effect and no environmental pollution.
Owner:广州舒国生物科技有限公司

Heterologous expression of fungal cellobiohydrolase 2 genes in yeast

ActiveUS9447398B2Promote productionAugment cellulose hydrolysisBiofuelsFermentationHeterologousBiotechnology
The present invention provides for heterologous expression of polypeptides encoded by wild-type and codon-optimized cbh2 genes from the organisms Cochliobolus heterostrophus, Gibberella zeae, Irpex lacteus, Volvariella volvacea, and Piromyces sp. in host cells, such as the yeast Saccharomyces cerevisiae. The expression in such host cells of the corresponding genes, and variants and combinations thereof, result in improved specific activity of the expressed cellobiohydrolases. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.
Owner:STELLENBOSCH UNIVERSITY

Saccharomycetes strain and application in cassava fermentation

The invention discloses saccharomycetes (Saccharomyces sp.)Ca-mic-26-3. The preservation number of the saccharomycetes is CGMCC No.18284. A microbial preparation is prepared from the saccharomycetes strain, the saccharomycetes strain is used for cassava whole powder fermentation, according to a generated cassava whole powder derivative, the structure characteristics are changed, the viscosity is effectively lowered, the gelatinization temperature is effectively lowered, the gelatinization time is reduced, the filling power is improved, the taste, the palatability and the like of products of the cassava whole powder derivative are improved, the cassava whole powder derivative can be a substitute of other starch, and using value of the cassava whole powder is improved.
Owner:GUANGXI SUBTROPICAL CROPS RES INST GUANGXI SUBTROPICAL AGRI PROD PROCESSING RES INST

Saccharomyces sp. HF-21 and method for preparing D-pantoic acid lactone through biphasic catalysis of Saccharomyces sp. HF-21

The invention relates to a Saccharomyces sp. HF21, which is collected in the China Center for Type Culture Collection (CCTCC) on June 17, 2021, and the collection number is CCTCC No. M 2021728. The invention develops a biphasic catalysis fermentation method, hydrolysis of ketopantoic acid in a water phase is inhibited, inactivation of the Saccharomyces sp. in an organic phase is avoided, and catalysis of the Saccharomyces sp. for a substrate on an organic phase-water phase two-phase interface is achieved.
Owner:HEFEI UNIV

Yeast for synthesizing 3-indolebutyric acid and application of yeast in 3-indolebutyric acid preparation

ActiveCN106967626AMild conditions for the preparation of IBA by fermentationFungiMicroorganism based processesBiotechnologyChemical synthesis
The invention discloses yeast with the function of synthesizing 3-indolebutyric acid and application of the yeast in 3-indolebutyric acid preparation, and solves problems of environmental pollution and complex chemical synthesis processes of 3-indolebutyric acid. The Latin name of the yeast with the function of synthesizing 3-indolebutyric acid is Saccharomyces sp., a depository authority is China general microbiological culture collection center, a depository date is April 11, 2017, and a depository serial number is CGMCC NO.14011. The yeast strain can convert IAA prepared by microbial fermentation into IBA, and fermentation liquid is filtered, concentrated and crystallized to obtain IBA.
Owner:SICHUAN LANYUE SCI & TECH

Probiotic composition, application of composition and auxiliary anti-alcoholism preparation

The invention belongs to the technical field of probiotics, and discloses a probiotic composition, application of the composition and an auxiliary hangover alleviating preparation. Lactobacillus casei powder, lactobacillus paracasei powder, lactobacillus acidophilus powder and lactobacillus rhamnosus powder are used as main components, and the lactobacillus paracasei, the lactobacillus acidophilus and the lactobacillus rhamnosus are used for conditioning intestinal tracts and relieving pressure for the liver; the lactobacillus casei greatly relieves the burden of the liver on alcohol infiltration; under cooperation of saccharomycetes, bifidobacterium and rehydration salt, decomposition of alcohol in the body is assisted, the content of endotoxin and toxic substances harmful to the liver is reduced, fat accumulation in the liver is reduced, intestinal leakage caused by alcohol is improved, damage, aging and inflammatory response of intestinal tracts and the liver are reduced, the liver is protected, and the morbidity of alcoholic fatty liver is reduced. And moisture and electrolyte are supplemented, so that sobering is faster. The auxiliary hangover alleviating preparation is convenient and easy to obtain, and can efficiently achieve the purposes of assisting in hangover alleviating and preventing and relieving hangover symptoms.
Owner:北京海思未来健康科技有限公司

Six divine comedies and preparation method of six divine comedies

The invention provides Liushenqu and a preparation method of Liushenqu. The purpose is to solve the technical problem of slow fermentation speed when preparing Liushenqu in the prior art. The adopted technical scheme is: the preparation method of Liushenqu includes the following steps: S1: pulverize bitter almond and red bean into powder, mix with flour and wheat bran to obtain pre-prepared powder; Decoct in water for 1 hour, take the filtrate and concentrate it into a clear paste; S2: mix the clear paste with the pre-powder to obtain a fermentation substrate; S3: sterilize the fermentation substrate; S4: combine the compound bacterial agent with the sterilized fermentation substrate S5: granulating and drying the fermented product to obtain Liushenqu; wherein, the compound bacterial agent includes: powdered miller's yeast, Rhizomucor parvum, Aureobacter alba, Monascus purpureus, Saccharomyces sp., Pichia burton. In addition, the present invention also provides Liushenqu prepared by the aforementioned preparation method.
Owner:SICHUAN BAISHENG PHARMA
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