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Separation and characterization method of ginseng polysaccharide with immunomodulatory and anti-tumor activity

A technology of ginseng polysaccharides and immune activity, applied in the field of separation and characterization of ginseng polysaccharides, to achieve the effects of promoting value-added ability, promoting inhibition, good immunity and anti-tumor activity

Active Publication Date: 2021-02-26
上海中盈经济发展(集团)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows us to determine if we have any benefits from certain compounds found naturally or synthetically produced by plants called ginsanpolymers (GPM). These molecules are able to stimulate specific parts of our body's own defense mechanisms against disease caused by harmful substances like bacteria or viruses. They may be used alone or combined together to create new materials useful for various applications such as medicine development or biomedical researches.

Problems solved by technology

This patented technical problem addressed in this patents relates to studying the properties of certain compounds called pinosylpolygensuccanosilicate polymers derived from plants like panaceans or licenses therapy powders containing them. These materials exhibiting similar structures include other substances present within the same genus Helicosapenta Laccina Benthisca extractive ingredients obtained from wild species belonging to the generics Group Iliposporaceae which contains five main active principles - ribbon sugars, carboxylic acids, hydroxyproline, amino acids and sugar alcohol.

Method used

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  • Separation and characterization method of ginseng polysaccharide with immunomodulatory and anti-tumor activity
  • Separation and characterization method of ginseng polysaccharide with immunomodulatory and anti-tumor activity
  • Separation and characterization method of ginseng polysaccharide with immunomodulatory and anti-tumor activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1 Extraction, Purification and Content Determination of Ginseng Crude Polysaccharide

[0057] The raw material is the rhizome of ginseng from Changbai Mountain in Jilin Province, which is washed, dried, and crushed into powder with a pulverizer for later use. Take 5g of ginseng powder, use water as the extractant, and use microwave extraction method to extract; microwave extraction time is 6min, temperature is 70°C, solid-liquid ratio is 1:30 (mg / ml), power is 550W, extract and concentrate , dried to obtain ginseng crude polysaccharide, named GSP.

[0058] DEAE-cellulose pretreatment: Soak DEAE-cellulose dry powder in distilled water first, soak in 0.5mol / L HCl solution for 1-2 hours, then wash with distilled water until the pH value of the filtrate is neutral, drain the The DEAE-cellulose was soaked in 0.5 mol / L NaOH solution for 1-2 hours, then washed with distilled water until the pH value of the filtrate was neutral, and then packed into a column.

[0059] C...

Embodiment 2

[0065] The separation of embodiment 2 ginseng polysaccharides

[0066] DEAE-52 loading and elution. Specifically, weigh the purified ginseng polysaccharide, dissolve it with 5mL of distilled water, and slowly add it along the wall of the column when loading the sample, so as to prevent the filler from flying up and causing uneven sample loading. The neutral sugar fraction was first eluted with distilled water; the acidic sugar fraction was sequentially eluted with 0.1mol / L, 0.2mol / L, and 0.3mol / L NaCl aqueous solution at a flow rate of 1mL / min, collected in separate bottles, and collected every 5 minutes One bottle, each bottle is 5ml. Use the phenol-sulfuric acid method to measure the absorbance of each bottle of liquid, take the serial number of the vial as the abscissa, and take the absorbance A as the ordinate to draw the elution curve. The results are as follows: figure 1 shown.

[0067] Such as figure 1 As shown, the elution curve was obtained after being purified by...

Embodiment 3

[0068] The structural characterization of embodiment 3 ginseng polysaccharide

[0069] 1. Measurement and analysis of ultraviolet spectrum

[0070] Take 2.00 mg of isolated ginseng polysaccharide and dissolve it in 2 ml of distilled water, then scan it with a UV spectrophotometer in the range of 200-800 nm, and record the UV absorption spectrum.

[0071] Ultraviolet analysis: the scanning range is in the range of 200-800nm. from figure 2 It can be seen from the figure that GSP-0 and GSP-1 detected protein absorption peaks, indicating that there may be glycoproteins in these two polysaccharides, which is consistent with the speculation of the physicochemical properties in the purification stage. from figure 2 It can be seen that, GSP-2 and GSP-3, the spectral curves are relatively smooth, indicating that protein and nucleic acid impurities in GSP-2 and GSP-3 have been reduced to a minimum or do not exist.

[0072] 2. Determination and analysis of infrared spectrum and sec...

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Abstract

The invention relates to the field of medicine development functional food, in particular to a characterization method of ginseng polysaccharide with immunoregulation and anti-tumor activity. The ginseng polysaccharide GSP is extracted through a microwave extraction method, cotton DEAE is adopted for purification, DEAE-52 column chromatography is adopted for further separation, and ginseng monomerpolysaccharide is obtained. Instruments such as UV, IR, HPLC, NMR and the like are adopted to characterize different components of the ginseng polysaccharide. Congo red experiments prove for the first time that the four components all have a triple helix structure. Infrared display shows that the GSP2 contains an alpha-type characteristic absorption peak; the second derivative analysis polysaccharide is formed by connecting alpha-glucosidic bonds and beta-glucosidic bonds. Immunoregulation and anti-tumor effects of the components are studied in vitro, and the GSP-2 component is good in immunocompetence, is expected to be used as a potential immunomodulator and is used for development of functional foods or medicines.

Description

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Claims

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Application Information

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Owner 上海中盈经济发展(集团)有限公司
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