SNP locus subtype optimization method for neural and mental disease genes
A gene locus and spirit technology, applied in the field of SNP locus typing optimization of neurological and psychiatric diseases, can solve the problems of high cost, harsh experimental conditions, and cumbersome experimental process of CAPs methods.
Pending Publication Date: 2021-02-26
广东瑞昊生物技术有限公司
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Problems solved by technology
However, the above methods all have certain shortcomings: SSCP can only use non-denatured glue, and the experimental conditions are harsh, requiring room temperature conditions, which are difficult to achieve in general laboratories; the experimental process of CAPs is cumbersome, and an experimental procedure takes a long time; MassARRAY mass spectrometry The cost of TaqMan probe method and other chip methods is quite high, and it is difficult for ordinary laboratories to afford
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Embodiment 1
[0114] A method for optimizing the SNP site typing of neurological and mental disease genes, such as figure 1 shown, including the following steps:
[0115] 1) Extract the genomic DNA in the sample to obtain a DNA extract;
[0116] 2) Take 1 μl DNA extract obtained in step 1) for PCR amplification, add 1 μl PCR buffer solution, 3.0mmol Mg 2+ Solution, 0.3mmol dNTP, 1U DNA polymerase (from Qiagen Inc.) and 1μl of the first PCR primer mixed reaction, then add 1μl PCR buffer solution (GC-I buffer from Takara. Company), 3.0mmol Mg 2+ solution, 0.3mmol dNTP, 1U DNA polymerase (from Qiagen Inc.) and 1μl of the second PCR primer to obtain the PCR amplification product; the amplification conditions are: 95°C, 5min pre-denaturation; then sequentially at 94°C, 20s, 65°C , 40s, 72°C, 1.5min, for 11 cycles; then at 94°C, 20s, 59°C, 30s, 72°C, 1.5min, for 20 cycles; then keep at 72°C for 2min; finally store at 4°C .
[0117] Among them, the first PCR primer is composed of 21 SNP gene l...
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The invention discloses an SNP locus subtype optimization method for neural and mental disease genes. The method comprises the following steps: 1) extracting DNA; 2) carrying out PCR amplification onthe DNA; 3) purifying a PCR amplification product; 4) extending the purified PCR amplification product; 5) purifying an extension product; 6) carrying out sequencing by a sequencer; and 7) analyzing afluorescent mark and length information on the extension product. According to the method, based on a technology SNaPshot, PCR amplification primers and extension primers of different lengths are designed for different SNP loci, thus, a plurality of SNP loci are subjected to typing in one reaction system, and typing of 21 loci can be carried out simultaneously. Due to four-color fluorescent marking, various SNP types can be classified, and meanwhile, insertion and deletion can be analyzed. After gene subtypes of neural and mental disease sufferers are analyzed, the condition that doctors prescribe a medicine special for diseases is facilitated.
Description
technical field [0001] The present invention relates to the technical field of gene identification, and in particular relates to a method for optimizing the SNP site typing of neurological and mental disease genes. Background technique [0002] Neuropathy refers to diseases of the nervous system that occur in the central nervous system, peripheral nervous system, and autonomic nervous system, and are mainly manifested by sensory, motor, consciousness, and autonomic dysfunction. The nervous system is related to the spirit. A person’s emotional problems can easily lead to diseases of the nervous system. The nervous system is also a part of the body and is closely related to other systems. When improving the nervous system, it can improve bad emotions. Physical fitness, to achieve the purpose of physical and mental health. [0003] Mental illness, also known as mental illness, refers to the disorder of brain function under the influence of various biological, psychological and...
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IPC IPC(8): C12Q1/6869C12Q1/6883
CPCC12Q1/6869C12Q1/6883C12Q2600/156C12Q2600/172C12Q2600/106C12Q2521/319C12Q2521/525C12Q2563/107C12Q2531/113
Inventor 廖华黄宇东米丁丹
Owner 广东瑞昊生物技术有限公司