Method for extracting effective component in verbena and application of effective component in dysmenorrhea treatment

An active ingredient, the technology of verbena, applied in a method and its application field in the treatment of dysmenorrhea, can solve the problems of waste of medicinal value of verbena, insufficient research on verbena, and no clear understanding of the effect, etc., and achieve low price , easy to popularize and realize the effect of industrialized production and low equipment cost

Inactive Publication Date: 2021-03-05
连江仁泽生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, people's research on verbena itself is not deep enough, and there is no clear understanding of its effect when used alone, which makes the medicinal value of verbena itself greatly wasted

Method used

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  • Method for extracting effective component in verbena and application of effective component in dysmenorrhea treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Take Verbena medicinal material, remove the root, dry and chop it, pass it to 20-30 mesh, add 5 times the amount of 80% ethanol to soak for 6 hours, heat to 50 ° C, reflux extraction 3 times, extract After 1 hour, the extracts were combined, the filtrate was collected after suction filtration, and the filtrate was concentrated under reduced pressure to produce an ethanol extract of verbena equivalent to 1 g / mL;

[0024] (2) Get 1 part of the Verbena ethanol extract, extract 3 times with 1 part of petroleum ether and 1 part of chloroform successively, combine the extracts to obtain the Verbena extract, after the Verbena extract is evaporated to dryness, water Dissolved to obtain a 1g / mL verbena extract liquid;

[0025] (3) Take 40mL of 1g / mL Verbena extract medicinal solution, add 500mL of water, and decoct with slow fire for 120 minutes to make an oral solution.

[0026] Usage and dosage: twice a day, 30mL each time.

Embodiment 2

[0028] (1) Take Verbena medicinal material, remove the root, dry it and chop it, pass through a 30-mesh sieve, add 10 times the amount of 90% ethanol to soak for 12 hours, heat to 60 ° C, reflux extraction 4 times, extraction time 2 hour, combined the extracts, collected the filtrate after suction filtration, concentrated the filtrate under reduced pressure, and made the Verbena ethanol extract equivalent to 2g / mL;

[0029] (2) Get 2 parts of the Verbena ethanol extract, extract 4 times with 1 part of ethyl acetate and 2 parts of n-butanol successively, combine the extracts to obtain the Verbena extract, and evaporate the Verbena extract to dryness Finally, dissolve in water to obtain the verbena extract liquid of 2g / mL;

[0030] (3) Take 40mL of 2g / mL Verbena extract liquid, add 500mL of water, decoct with slow fire for 120 minutes, and make oral liquid.

[0031] Usage and dosage: twice a day, 30mL each time.

Embodiment 3

[0033] (1) Take Verbena medicinal material, remove the root, dry and chop it, pass it to 20-30 mesh, add 5 times the amount of 80% ethanol to soak for 6 hours, heat to 50 ° C, reflux extraction 3 times, extract After 1 hour, the extracts were combined, the filtrate was collected after suction filtration, and the filtrate was concentrated under reduced pressure to produce an ethanol extract of verbena equivalent to 1 g / mL;

[0034] (2) Get 1 part of the Verbena ethanol extract, extract 3 times with 1 part of petroleum ether and 1 part of n-butanol successively, combine the extracts to obtain the Verbena extract, and evaporate the Verbena extract to dryness , dissolved in water to obtain a 1g / mL verbena extract liquid;

[0035] (3) Take 40mL of 1g / mL Verbena extract liquid, add 100mL of water, decoct with slow fire for 120 minutes, cool to room temperature naturally, add dextrin to a total of 500g, mix, granulate, dry, pass 60-mesh sieve, whole grain, packaged, 10g per bag, mad...

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Abstract

The invention discloses a method for extracting an effective component in verbena and application of the effective component in dysmenorrhea treatment. The preparation method comprises the following steps that (1) verbena medicinal materials are taken, after roots are removed, the medicinal materials are dried and cut into 20-30 meshes, 5-10 times of 80%-90% ethyl alcohol is added for soaking for6-12 hours, heating is performed to 50-60 DEG C, reflux extraction is performed 3-4 times, extraction time is 1-2 hours, extraction solutions are combined, a filtrates is collected after suction filtration, and the filtrate is subjected to pressure reduction concentration to prepare a verbena ethanol extract of 1-2 g / mL; and (2) 1-2 parts of the verbena ethanol extracts are taken, extraction is sequentially conducted for 3-4 times by using 1-2 parts of different extraction agents, extraction solutions are combined to obtain a verbena extract, and after the verbena extract is evaporated and dried, dissolving is conducted with water to obtain verbena extract liquid medicine of 1-2 g / mL. The verbena extract liquid medicine prepared through the method has definite treatment and relieving effects on dysmenorrhea, and can be used for preparing medicine for treating dysmenorrhea.

Description

technical field [0001] The invention relates to the technical field of pharmacy, in particular to a method for extracting active ingredients in verbena and its application in treating dysmenorrhea. Background technique [0002] Verbena is the dry aerial part of Verbenaceae plant Verbena, which was recorded in the 2010 edition of "Chinese Pharmacopoeia" (Part One). It has the effects of promoting blood circulation, detoxification, diuresis, jaundice, and cutting. Clinically, it is mostly used for the treatment of pain accumulation, dysmenorrhea, amenorrhea, sore throat, pain swelling, edema, yellow plague, and malaria. [0003] Dysmenorrhea refers to lower abdominal pain, bloating, accompanied by backache or other discomforts before and after menstruation or during menstruation. Traditional Chinese medicine believes that the onset of dysmenorrhea is related to factors such as congenital endowment, physical condition, emotion, fatigue, exposure to cold, and sudden changes in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/85A61P29/00A61P15/00
CPCA61K36/85A61K2236/333A61K2236/39A61K2236/55A61P15/00A61P29/00
Inventor 曾鹏程
Owner 连江仁泽生物科技有限公司
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