A rapid and accurate method for colorimetric detection of heavy metal ions

A technology of heavy metal ions and metal ions, which is applied in the field of analysis and detection, can solve the problems of complicated operation and difficult preparation of monoclonal antibodies, and achieve the effect of simple and accurate detection methods.

Active Publication Date: 2022-05-06
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, heavy metal ions themselves are not suitable as antigens to stimulate organisms to produce antibodies, and they need to be complexed. In addition, the preparation of monoclonal antibodies required in the detection is difficult and the operation is relatively complicated.

Method used

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  • A rapid and accurate method for colorimetric detection of heavy metal ions
  • A rapid and accurate method for colorimetric detection of heavy metal ions
  • A rapid and accurate method for colorimetric detection of heavy metal ions

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Experimental program
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Effect test

Embodiment 1

[0056] Embodiment 1: A kind of method for rapid detection of heavy metal ions

[0057] A method for rapid detection of metal ions, said method is based on bathocuproine (BCP), xylenol orange (XO), diphenylcarbazide (DPC), thio-Michler's ketone (TMK), cadmium reagent (Cadion ) and Victoria Blue B (VBB) reagent six kinds of complexing reagents are combined into a complexed colorimetric array sensor, respectively, the sample to be tested is added to bathocuproine (BCP), xylenol orange (XO), diphenylsemicarbazide (DPC), thio-Michler's ketone (TMK), cadmium reagent (Cadion) and Victoria blue B (VBB) reagents are mixed and reacted and then scanned to obtain the spectral data of the liquid to be tested, and linear discriminant LDA analysis is performed on the spectral data , by comparing the positions of the data points of the unknown sample in the LDA spectrum with the data points of the known types of metal ions, the type, composition and content of the metal ions in the liquid to ...

Embodiment 2

[0080] Embodiment 2: a kind of for detecting Pb 2+ Complexation Colorimetric Array Sensing

[0081] One for detection of Pb 2+ The complexation colorimetric array sensing of the described complexation colorimetric array sensing is made up of two kinds of complexation reagents of XO and VBB.

[0082] Xylenol orange (XO) system:

[0083] 50 μM XO, 5 mM o-phenanthroline, HAC-NaAC buffer solution (pH 5.5), 20 μM Pb 2+ After mixing, observe its color change after standing for 5 minutes, and the solution turns from yellow to light red. The mixed solution was scanned with a microplate reader to obtain absorbance values ​​at characteristic absorption (maximum absorption wavelengths of 574nm and 435nm) of samples with different concentrations.

[0084] Victoria Blue B (VBB) system:

[0085] Mix 100 mM KI, 0.8 mM ascorbic acid and 70 μM VBB with 20 μM Cd 2+ After mixing and mixing, observe its color change after standing for 5 minutes, and the solution turns from green to blue. T...

Embodiment 3

[0086] Embodiment 3: a kind of for detecting Cu 2+ Complexation Colorimetric Array Sensing

[0087] A method for detecting Cu 2+ The complexation colorimetric array sensor of the described complexation colorimetric array sensor is made up of BCP and VBB reagent.

[0088] Bath copper spirit (BCP) system:

[0089] 0.5 mM BCP solution, 60 mM hydroxylamine hydrochloride solution and 20 μM Cu 2+ After mixing, observe its color change after standing for 5 minutes, and the solution changes from colorless to light yellow. The mixed solution was scanned with a microplate reader to obtain absorbance values ​​at characteristic absorption (maximum absorption wavelength 475 nm) of samples with different concentrations.

[0090] Victoria Blue B (VBB) system:

[0091] Mix 100 mM KI, 0.8 mM ascorbic acid and 70 μM VBB with 20 μM Cd 2+ After mixing and mixing, observe its color change after standing for 5 minutes, and the solution turns from green to blue. The mixed solution was scanned...

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Abstract

The invention discloses a method for rapidly and accurately colorimetrically detecting heavy metal ions, and belongs to the technical field of analysis and detection. The method is to scan the absorption spectrum after the complexation colorimetric array sensor is mixed with the sample to be tested to obtain the spectral data of the liquid to be tested, perform linear discriminant LDA analysis on the spectral data, and analyze the unknown sample data points in the LDA spectrum The position is compared with the data points of known types of metal ions to determine the type, composition and content of the metal ions in the liquid to be tested; the complexation colorimetric array sensing refers to a combination of multiple A colorimetric array sensor composed of complexing reagents for color reaction; the heavy metal ions include Hg 2+ 、Cd 2+ , Pb 2+ and Cu 2+ one or more of. The method of the present invention is to Pb 2+ , Hg 2+ , Cu 2+ and Cd 2+ The four ions can be detected simultaneously and at low concentrations, and the detection method is simple and accurate, with an accuracy rate of 100%.

Description

technical field [0001] The invention relates to a method for fast and accurate colorimetric detection of heavy metal ions, belonging to the technical field of analysis and detection. Background technique [0002] Heavy metals usually refer to metal substances whose specific gravity is greater than 5, and can also be defined as density greater than 4.5g / cm 3 Metal. There are 45 kinds of gold, silver and copper that meet this definition, some of which, such as zinc and magnesium, are essential elements for the human body, but from the perspective of pollution, most of them refer to lead, cadmium, mercury, etc. that are significantly toxic. In the past 20 years, my country's industry has developed rapidly, and more and more industries have produced heavy metal emissions, such as metals, smelting, chemicals, printing and dyeing, etc. In recent years, the IT industry has been emerging, and some enterprises in the Yangtze River Delta and Pearl River Delta regions have discharged...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/78G01N21/3103
Inventor 沈晓芳于丽洪庞月红
Owner JIANGNAN UNIV
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