A rapid and accurate method for colorimetric detection of heavy metal ions
A technology of heavy metal ions and metal ions, which is applied in the field of analysis and detection, can solve the problems of complicated operation and difficult preparation of monoclonal antibodies, and achieve the effect of simple and accurate detection methods.
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Embodiment 1
[0056] Embodiment 1: A kind of method for rapid detection of heavy metal ions
[0057] A method for rapid detection of metal ions, said method is based on bathocuproine (BCP), xylenol orange (XO), diphenylcarbazide (DPC), thio-Michler's ketone (TMK), cadmium reagent (Cadion ) and Victoria Blue B (VBB) reagent six kinds of complexing reagents are combined into a complexed colorimetric array sensor, respectively, the sample to be tested is added to bathocuproine (BCP), xylenol orange (XO), diphenylsemicarbazide (DPC), thio-Michler's ketone (TMK), cadmium reagent (Cadion) and Victoria blue B (VBB) reagents are mixed and reacted and then scanned to obtain the spectral data of the liquid to be tested, and linear discriminant LDA analysis is performed on the spectral data , by comparing the positions of the data points of the unknown sample in the LDA spectrum with the data points of the known types of metal ions, the type, composition and content of the metal ions in the liquid to ...
Embodiment 2
[0080] Embodiment 2: a kind of for detecting Pb 2+ Complexation Colorimetric Array Sensing
[0081] One for detection of Pb 2+ The complexation colorimetric array sensing of the described complexation colorimetric array sensing is made up of two kinds of complexation reagents of XO and VBB.
[0082] Xylenol orange (XO) system:
[0083] 50 μM XO, 5 mM o-phenanthroline, HAC-NaAC buffer solution (pH 5.5), 20 μM Pb 2+ After mixing, observe its color change after standing for 5 minutes, and the solution turns from yellow to light red. The mixed solution was scanned with a microplate reader to obtain absorbance values at characteristic absorption (maximum absorption wavelengths of 574nm and 435nm) of samples with different concentrations.
[0084] Victoria Blue B (VBB) system:
[0085] Mix 100 mM KI, 0.8 mM ascorbic acid and 70 μM VBB with 20 μM Cd 2+ After mixing and mixing, observe its color change after standing for 5 minutes, and the solution turns from green to blue. T...
Embodiment 3
[0086] Embodiment 3: a kind of for detecting Cu 2+ Complexation Colorimetric Array Sensing
[0087] A method for detecting Cu 2+ The complexation colorimetric array sensor of the described complexation colorimetric array sensor is made up of BCP and VBB reagent.
[0088] Bath copper spirit (BCP) system:
[0089] 0.5 mM BCP solution, 60 mM hydroxylamine hydrochloride solution and 20 μM Cu 2+ After mixing, observe its color change after standing for 5 minutes, and the solution changes from colorless to light yellow. The mixed solution was scanned with a microplate reader to obtain absorbance values at characteristic absorption (maximum absorption wavelength 475 nm) of samples with different concentrations.
[0090] Victoria Blue B (VBB) system:
[0091] Mix 100 mM KI, 0.8 mM ascorbic acid and 70 μM VBB with 20 μM Cd 2+ After mixing and mixing, observe its color change after standing for 5 minutes, and the solution turns from green to blue. The mixed solution was scanned...
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