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Sea antler fucoidan and application thereof in preparation of medicines and functional products for preventing and treating leucopenia

A technology of sea velvet fucoidan sulfate and leukopenia, applied in the field of marine medicine, can solve the problems of high price and unsatisfactory curative effect, and achieve the effects of promoting proliferation, increasing the number of bone marrow nucleated cells, and improving the formation of

Active Publication Date: 2021-04-02
MARINE BIOMEDICAL RES INST OF QINGDAO CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the disadvantages of unsatisfactory curative effect and high price of drugs for treating leukopenia in the prior art, the present invention provides a kind of sea antler fucoidan sulfate and its use in the preparation of drugs and functional products for preventing and treating leukopenia. application

Method used

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  • Sea antler fucoidan and application thereof in preparation of medicines and functional products for preventing and treating leucopenia
  • Sea antler fucoidan and application thereof in preparation of medicines and functional products for preventing and treating leucopenia
  • Sea antler fucoidan and application thereof in preparation of medicines and functional products for preventing and treating leucopenia

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Experimental program
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Effect test

Embodiment 1

[0038] Embodiment 1, the preparation of sea antler fucoidan sulfate ester (method 1)

[0039] Wash the sea velvet, dry it, crush it, and pass it through a 20-mesh sieve. Accurately weigh 200 g of algae powder, add 2 L of 0.05 mol / L dilute hydrochloric acid according to the solid-liquid ratio of 1:10, and extract at 60 ° C for 4 h. Centrifuge the extract at 4000rpm for 15min, collect the supernatant, and remove the algae residue. The extracted supernatant was concentrated under reduced pressure to 1 / 5 of the total volume, and 95% ethanol was added thereto until the ethanol concentration was 20%, and left standing overnight to obtain the precipitated fraction HR-1. Continue to add 95% ethanol to the supernatant to a final concentration of 70%, let it stand overnight, and dry the obtained precipitate in an oven at 50° C. to obtain crude polysaccharide. The crude polysaccharide is prepared into an aqueous solution with a concentration of 2%, and after being classified by a 30kDa...

Embodiment 2

[0040] Embodiment 2, the preparation of sea antler fucoidan sulfate ester (method 2)

[0041] Wash the sea velvet, dry it, crush it, and pass it through a 20-mesh sieve. Accurately weigh 200g of algae powder, add 0.1mol / L CaCl according to the ratio of material to liquid 1:20 22L, extract at 70°C for 4h. Centrifuge the extract at 4000rpm for 15min, collect the supernatant, and remove the algae residue. The extracted supernatant was concentrated under reduced pressure to 1 / 5 of the total volume, 95% ethanol was added thereto until the ethanol concentration was 20%, and the precipitate was left standing overnight. Continue to add 95% ethanol to the supernatant to a final concentration of 70%, let it stand overnight, and dry the obtained precipitate in an oven at 50° C. to obtain crude polysaccharide. The crude polysaccharide is formulated into an aqueous solution with a concentration of 2%, and after being classified by a 30kDa ultrafiltration membrane, the filtrate is concen...

Embodiment 3

[0042] Embodiment 3, the preparation of sea antler fucoidan sulfate ester (method 3)

[0043] Wash the sea velvet, dry it, crush it, and pass it through a 20-mesh sieve. Accurately weigh 200g of algae powder, add 2L of 0.2mol / L calcium chloride-0.05mol / L dilute hydrochloric acid mixed solution according to the solid-liquid ratio of 1:10, and extract at 50°C for 4h. Centrifuge the extract at 4000rpm for 15min, collect the supernatant, and remove the algae residue. The extracted supernatant was concentrated under reduced pressure to 1 / 5 of the total volume, 95% ethanol was added thereto until the ethanol concentration was 30%, and the precipitate was left standing overnight. Continue to add 95% ethanol to the supernatant to a final concentration of 80%, let stand overnight, and dry the obtained precipitate in an oven at 50° C. to obtain crude polysaccharide. The crude polysaccharide was passed through an anion-exchange chromatography column QSepharose Fast Flow, and the concen...

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Abstract

The invention provides a sea antler fucoidan and an application thereof in preparation of medicines and functional products for preventing and treating leucopenia. Cell experiments prove that fucoidancan significantly promote the formation of granulocyte-macrophage hematopoietic progenitor cell colonies, and also can promote the proliferation of bone marrow stromal cells. In-vivo experiments further show that the fucoidan can obviously inhibit mice leukocyte reduction, neutrophil reduction and thrombocytopenia caused by cyclophosphamide, and increase the number of bone marrow nucleated cellsof mice. The fucoidan provided by the invention has the effect of antagonizing myelosuppression caused by chemotherapeutics, and can be used as a functional component for developing medicines and functional products for preventing and treating leucopenia.

Description

technical field [0001] The invention belongs to the field of marine medicines, and in particular relates to sea antler fucoidan sulfate and its application in the preparation of medicines and functional products for preventing and treating leukopenia. [0002] technical background [0003] Leukopenia (Leukopenia) is a common side effect of tumor radiotherapy and chemotherapy, which refers to the number of white blood cells in the peripheral blood continuously lower than 4×10 9 a / L. If not treated in time, severe infection and complications may occur, which seriously threaten human health. Therefore, resistance to leukopenia caused by cancer treatment is a key factor in improving the quality of anticancer treatment. At present, the clinical drugs for the treatment of leukopenia mainly include granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), adenine, batyl alcohol, etc. However, their application Accompanied by high tr...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P7/00
CPCC08B37/0063C08B37/0003A61P7/00
Inventor 胡婷赵峡于广利李建杰杨英杰管华诗辛萌李全才吕友晶
Owner MARINE BIOMEDICAL RES INST OF QINGDAO CO LTD
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