Application of stilbene glucoside compound in preparation of medicine for preventing and/or treating coronavirus infection diseases

A coronavirus, infectious disease technology, applied in antiviral agents, drug combinations, respiratory diseases, etc., can solve the problem of lack of coronavirus coronavirus S protein and ACE2 receptor binding drugs, etc. Therapeutic, fast absorption, direct effect of pulmonary administration

Inactive Publication Date: 2021-10-12
昆明翔昊科技有限公司
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The application provides the application of a stilbene compound in the preparation of drugs for the prevention and / or treatment of coronavirus infection, which is used to solve the problem in the prior art that can effectively inhibit coronavirus 3CL protease, inhibit coronavirus S protein and ACE2 receptors. Technical issues with body-bound drugs

Method used

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  • Application of stilbene glucoside compound in preparation of medicine for preventing and/or treating coronavirus infection diseases
  • Application of stilbene glucoside compound in preparation of medicine for preventing and/or treating coronavirus infection diseases
  • Application of stilbene glucoside compound in preparation of medicine for preventing and/or treating coronavirus infection diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The preparation of embodiment 1 tristilbene glycoside

[0056] Take 2000g of Lhasa rhubarb crude powder, reflux extraction with 16L of 95% ethanol for 3 times, 2 hours each time, filter, combine the filtrates, concentrate to dryness under reduced pressure, add 2000ml of water to the residue and stir to dissolve. Measure 1600m1 of the liquid supernatant, load the sample onto D101 macroporous adsorption resin, wash 5-6 times the column volume with water, then flush the column with 25% ethanol aqueous solution, collect 2 times the volume of the effluent, mix and concentrate, 15 % ethanol repeated recrystallization to obtain an extract containing 99% tristilbeside.

Embodiment 2

[0057] The preparation of embodiment 2 sargenin A

[0058] Include the following steps:

[0059] 1) After pulverizing the dried rhizome of Rhubarb Zangbian, mix it with 75% ethanol which is 10 times of the rhizome quality of rhubarb, and make a mixed liquid whose particle size is 0.5mm;

[0060] 2) coarse filtration: carrying out the filter diameter of mixed solution is the filtrate of 0.2mm;

[0061] 3) Fine filtration: the filtrate obtained by step 2) was centrifuged for 5 minutes with a rotating speed of 3000rpm and a centrifugal radius of 8cm to obtain an extract containing sarcinxanthin A.

[0062] 4) Extracted by D101 macroporous resin column chromatography, the sample loading flow rate is 1BV / h, and the distilled water is eluted at a flow rate of 2BV / h, and then eluted with a volume concentration of 35% ethanol solution, ethanol eluted The flow rate was 1BV / h, the collected ethanol eluate was concentrated under reduced pressure, and recrystallized with 30% ethanol to ...

Embodiment 3

[0063] The preparation of embodiment 3 Polygonum multiflorum stilbene glycosides

[0064] 1) Pulverize Radix Polygoni Multiflori to 80 mesh, add ethanol solution with a volume concentration of 60% according to the ratio of material to liquid ratio of 1g:100mL, and extract under reflux at 30°C for 1 hour to obtain an ethanol extract;

[0065] 2) The ethanol extract is recovered under reduced pressure to an extract with a relative density of 1.0 at 40° C., the extract is dissolved in water 10 times its volume, filtered, and the filtrate is taken for subsequent use;

[0066] 3) Put the filtrate on the S-8+ macroporous resin, the flow rate of sample loading is 1BV / h, first elute with distilled water, the elution flow rate of distilled water is 2BV / h, then elute with 50% ethanol solution with volume concentration, wash with ethanol The flow rate is 1BV / h, the ethanol eluate is collected, recrystallized with 30% ethanol to obtain the extract containing 99% stilbene glycosides of Polyg...

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Abstract

The invention discloses application of a stilbene glucoside compound in preparation of a medicine for preventing and/or treating coronavirus infection diseases. The medicine prepared from the stilbene glucoside compound shows an inhibition effect on the SARS-CoV 3CL protease activity and an effect of inhibiting combination of an SARS-CoV S protein and host cells ACE2. Meanwhile, the medicine also has the effects of preventing and/or relieving cytokine storm caused by coronavirus infection and preventing and/or relieving acute inflammatory injury of multiple organs caused by coronavirus infection.

Description

technical field [0001] The application relates to the application of a stilbene glycoside compound in the preparation of a drug for preventing and / or treating a coronavirus infection disease, belonging to the field of drugs for a coronavirus infection disease. Background technique [0002] Coronavirus (Coronaviridae) has the tropism of the gastrointestinal tract, respiratory tract and nervous system, and it has repeatedly caused severe acute respiratory syndrome that has ravaged the world, such as severe acute respiratory syndrome (Severe Acute Respiratory Syndrome, SARS, infectious disease) from the winter of 2002 to the spring of 2003. SARS), the Middle East Respiratory Syndrome (MERS) in 2012, and the recent global outbreak of novel coronavirus (2019-nCoV, SARS-CoV-2, CoVID-19) outbreaks. The infection outbreak of CoVID-19 has now been classified as a public health emergency of international concern. Coronavirus outbreaks occur every winter and spring, which has a huge i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7034A61P31/14A61P11/00
CPCA61K31/7034A61P31/14A61P11/00
Inventor 胡琳任杨帆李清清胡群尹开云方震东
Owner 昆明翔昊科技有限公司
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