Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of CDC20 in osteogenic differentiation of mesenchymal stem cells

A technology of 1. CDC20 and mesenchymal stem cells, applied in the field of stem cells, can solve problems such as increased expression and unclear effect of CDC20 on bone formation

Active Publication Date: 2021-04-09
PEKING UNIV SCHOOL OF STOMATOLOGY
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many studies have reported the biological functions of CDC20 in brain development, cilia disassembly and regulation of apoptosis, and the expression of CDC20 is elevated in many cancers, which are associated with poor prognosis in lung, bladder, colon, breast cancer and oral squamous cell carcinoma Related, but role of CDC20 on bone formation is unclear

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of CDC20 in osteogenic differentiation of mesenchymal stem cells
  • Application of CDC20 in osteogenic differentiation of mesenchymal stem cells
  • Application of CDC20 in osteogenic differentiation of mesenchymal stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Changes in the expression level of CDC20 during the osteogenic differentiation of human bone marrow mesenchymal stem cells

[0032] Cell culture:

[0033] Human bone marrow mesenchymal stem cells were purchased from ScienCell Research Laboratories (Carlsbad, CA, USA). Human bone marrow mesenchymal stem cells were collected from three different donors and were approved by the Ethics Committee of Peking University Stomatological Hospital. Proliferation medium is α-MEM medium (including 10% fetal bovine serum, 100 U / ml penicillin, 100 μg / ml streptomycin). Osteogenic differentiation medium includes proliferation medium plus 0.2mM vitamin C, 10mM sodium β-glycerophosphate and 100nM dexamethasone. Cells were seeded in culture dishes at 37°C, 5% CO 2 Cultured under conditions, the medium was changed once every 3 days. When the cells grew to 80% confluent state under an inverted microscope, they were digested and passaged with 0.25% trypsin, and added to the mediu...

Embodiment 2

[0062] Effects of inhibiting or overexpressing CDC20 on the osteogenic differentiation of human bone marrow mesenchymal stem cells

[0063] Plasmid expression, siRNA and lentiviral transfection:

[0064] The overexpression plasmid vector Vector and the overexpression CDC20 plasmid WT-CDC20 were purchased from ADDGENE Company. When the cells grew to 70%-80% confluence, the plasmid was transferred into the mesenchymal stem cells using the transfection reagent Lipo3000 according to the instructions, and the cells were collected after 24-36 hours for subsequent experiments. siRNA (small interfering RNA) was used to silence the expression of genes. The control siRNA and CDC20si were purchased from Shanghai Gemma Company. For mesenchymal stem cells, cells were collected at an appropriate time for subsequent experiments. The lentivirus can be integrated into the genome for gene interference. The knockdown control and CDC20sh were purchased from Shanghai Gemma Company. 72h after tr...

Embodiment 3

[0096] Knockdown of CDC20 regulates downstream genes of NF-κB pathway

[0097] Experiments were performed using qRT-PCR methods as described above. The primer information used for amplification is as follows:

[0098]

[0099]

[0100] image 3 It shows that the low expression of CDC20 provided by the embodiment of the invention promotes the high expression of NF-κB downstream genes. image 3 A-3D shows that after TNF-α was treated for 0.5 hours, the qRT-PCR results showed that the expression levels of NF-κB downstream genes IL-8, IL-6, and ICAM1 in the CDC20 knockdown group were significantly increased.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an application of CDC20 in an osteogenic differentiation process of mesenchymal stem cells. According to the invention, CDC20 has the effect of positively regulating and controlling an osteogenesis function of the mesenchymal stem cells, an application basis of CDC20 in the process of promoting osteogenic differentiation of the mesenchymal stem cells is provided, and the action mechanism of CDC20 on degradation of NF-kappa B active subunits p65 through a proteasome pathway is identified. The brand-new theoretical and experimental basis is provided for inhibiting activity of the target protein by using a small molecular compound in a targeted manner in the aspect of molecular treatment of bone regeneration.

Description

technical field [0001] The invention relates to the technical field of stem cells, in particular to the application of CDC20 in the osteogenic differentiation of mesenchymal stem cells. Background technique [0002] Bone defects caused by trauma, congenital malformations, infection, and tumors require bone tissue regeneration and reconstruction. As the population ages, there is an increasing need for regenerative repair of bone defects. In recent years, the application of mesenchymal stem cells combined with tissue engineering technology to promote tissue regeneration has become a frontier and important scientific issue. Human mesenchymal stem cells are an attractive source of adult stem cells in bone tissue engineering technology, and have important application value and good application prospects in the repair of various bone defects and bone regeneration in the oral and maxillofacial region. Human mesenchymal stem cells have the potential to differentiate into bone, car...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077C12N5/0775
CPCC12N5/0654C12N2501/405C12N2506/1353
Inventor 周永胜张萍杜杨格刘云松吕珑薇张晓张敏
Owner PEKING UNIV SCHOOL OF STOMATOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com