Application of gastrodin in the use of titanium metal in traditional Chinese medicine in diabetic environment

A technology of diabetes and gastrodin, applied in the fields of pharmaceutical formula, medical science, prosthesis, etc., can solve problems such as poor osseointegration of titanium metal implants, affect cell biological functions, oxidative stress damage, etc., and achieve enhanced osteogenic differentiation ability, increased proliferation level, and improved biological function

Active Publication Date: 2021-12-14
GENERAL HOSPITAL OF THE NORTHERN WAR ZONE OF THE CHINESE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Purpose of the invention: the present invention provides an application of gastrodin in the use of titanium metal implants in traditional Chinese medicine in diabetic environments, and its purpose is to solve the oxidative stress damage caused by diabetic environments to osteoblasts at the "titanium-bone" interface in the prior art , affect the biological function of cells and aggravate the damage of apoptosis, and finally lead to the clinical problems of poor osseointegration and long-term loosening of titanium metal implants, so as to improve the stability of medical titanium metal implants

Method used

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  • Application of gastrodin in the use of titanium metal in traditional Chinese medicine in diabetic environment
  • Application of gastrodin in the use of titanium metal in traditional Chinese medicine in diabetic environment
  • Application of gastrodin in the use of titanium metal in traditional Chinese medicine in diabetic environment

Examples

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Effect test

Embodiment 1

[0041] This example shows the application of gastrodin in the preparation of drugs for improving the adhesion function of osteoblasts at the "titanium-bone" interface in a diabetic environment, that is, the application of gastrodin in improving the adhesion state of osteoblasts on the titanium metal surface in a diabetic environment effect.

[0042] Experimental method: cutting and processing medical titanium metal into titanium sheets, grinding and polishing the surface, cleaning and disinfecting for later use. Use trypsin to isolate and culture the primary osteoblasts from the calvaria of SD rat suckling rats and pass them down, and press 1×10 4 Cells per milliliter were seeded on the surface of titanium metal with the same number to establish an in vitro "titanium-bone" interface model.

[0043] Divided into 5 groups:

[0044] 1) normal serum group (normal group);

[0045] 2) diabetes serogroup (diabetes group);

[0046] 3) Diabetes + gastrodin 1 μM;

[0047] 4) Diabet...

Embodiment 2

[0053] This example gives the application of gastrodin in the preparation of drugs that improve the proliferation and differentiation of osteoblasts at the "titanium-bone" interface in the diabetic environment, that is, the effect of gastrodin on the osteoblasts in the diabetic environment on the titanium metal surface is given. Improvement of proliferation ability and osteogenic differentiation level.

[0054] Experimental method: Osteoblasts were seeded on the surface of the titanium sheet for culture, and the grouping was the same as in Example 1. On the 4th and 7th days of culture, the cells on the surface of the titanium sheet were detected by MTT, and on the 7th day of culture, the cells on the surface of the titanium sheet were detected by ALP.

[0055] Result: if Figure 4 , Figure 5 As shown, compared with the normal control group, the proliferation and differentiation of osteoblasts in diabetic patients were significantly inhibited, and after adding gastrodin to d...

Embodiment 3

[0058] This example shows the application of gastrodin in the preparation of drugs for inhibiting the pathological apoptosis of osteoblasts at the "titanium-bone" interface in a diabetic environment, that is, it shows that gastrodin can reduce the apoptotic damage of osteoblasts on the titanium metal surface in a diabetic environment protective effect.

[0059] Experimental method: Osteoblasts were seeded on the surface of titanium metal for culture, and the grouping was the same as in Example 1. On the 7th day of culture, TUNEL and DAPI were used for double-labeled staining, and confocal microscope was used for observation; Caspase-3 kit was used to detect the activity of apoptosis pathway protein Caspase-3, and the effect of gastrodin on osteoblast apoptosis in diabetic environment was evaluated Impact.

[0060] result: Figure 6 The staining results of apoptotic cells in each group are shown, and the green image is the apoptotic cell nucleus marked by TUNEL. Figure 7 Fo...

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Abstract

The use of gastrodin in the use of titanium metal implants in the diabetic setting. After medical materials are implanted into the human body, the tissue repair at the "material-bone" interface mainly involves the integration of new bone tissue and materials, in which the bioactivity and function of osteoblasts responsible for osteogenic differentiation determine the effect of material osseointegration . The present invention establishes an in vitro "titanium-bone" interface model by attaching and culturing osteoblasts on the surface of medical titanium metal, and uses a therapeutic dose of gastrodin for additional culture, which proves that gastrodin can affect the osteogenesis of the "titanium-bone" interface in a diabetic environment Improvement of cell biological function. The present invention solves the oxidative stress damage caused by the diabetes environment on the "titanium-bone" interface osteoblasts in the prior art, affects the biological function of the cells and aggravates the apoptosis damage, and finally leads to poor osseointegration and long-term occurrence of titanium metal implants Clinical problems of loosening to improve the stability of medical titanium implants.

Description

technical field [0001] The invention belongs to the field of medical biomaterials, and specifically relates to the application of gastrodin in improving the osseointegration effect and long-term stability of medical titanium metal in diabetes environment. Background technique [0002] Titanium is the most widely used bone implant material so far. However, in some people with systemic metabolic diseases such as diabetes, the long-term instability rate of titanium implants is significantly increased. Studies have shown that the loosening rate of spinal screws in diabetic patients is as high as 30%, which is 10 times that of normal people. The long-term instability of titanium metal implants has caused great family and social burdens to patients. With the increasing number of diabetic patients, the demand for internal fixation of such patients with titanium implants is also increasing. Therefore, it is an urgent clinical problem to elucidate the mechanism of titanium metal im...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/54A61L27/06A61L27/28
CPCA61L27/54A61L27/06A61L27/28A61L2300/232A61L2400/06A61L2300/412A61L2300/40A61L2300/606A61L2300/602A61L2420/04A61L2420/02
Inventor 马翔宇刘兵项良碧周大鹏马一鸣
Owner GENERAL HOSPITAL OF THE NORTHERN WAR ZONE OF THE CHINESE PEOPLES LIBERATION ARMY
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