Construction and detection method of characteristic map of Boziren medicinal materials, decoction pieces, standard decoction and formula granules
A technology of formula granules and feature map, applied in the field of feature map construction, can solve the problems of inability to detect components, low accuracy, poor repeatability of test results, etc., and achieve stable overall quality, high accuracy, and feature peak information. rich effects
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Embodiment 1
[0082] A method for constructing a characteristic map of Boziren medicinal materials, decoction pieces, standard decoction and formula granules, comprising the following steps:
[0083] A. Preparation of reference substance solution: respectively take α-linolenic acid reference substance and linoleic acid reference substance, add methanol to dissolve, and make a solution containing 0.2 mg per 1 mL, that is;
[0084] B. Preparation of the test solution: take the medicinal material of Boziren, decoction pieces, standard decoction or / and formula granules, add methanol, and ultrasonically process to obtain;
[0085] C. Determination: respectively take the obtained reference substance solution and the obtained test solution, 10 μL of each, and inject them into a high performance liquid chromatograph, measure, and establish an HPLC-ELSD characteristic map;
[0086] The measurement conditions in the high performance liquid chromatograph meet:
[0087] Chromatographic column: with oc...
Embodiment 2
[0101] A method for detecting the characteristic spectrum of Boziren medicinal materials, decoction pieces, standard decoction and formula granules, comprising the following steps:
[0102] S1. Preparation of Boziren medicinal material test solution: take Boziren medicinal material powder, pass through No. 2 sieve, weigh 0.5 g, put it in a stoppered conical flask, add 10 mL of methanol, weigh it, and ultrasonically treat it (power 600W, Frequency 40kHz) 30min, let it cool, weigh the weight again, supplement the lost weight with methanol, shake well, filter, and take the continuous filtrate, that is;
[0103] S2. Preparation of Boziren decoction piece test solution: take Boziren decoction piece powder, pass through No. 2 sieve, weigh 0.5 g, put it in a stoppered conical flask, add 10 mL of methanol, weigh it, and ultrasonically treat it (power 600W, Frequency 40kHz) 30min, let it cool, weigh the weight again, supplement the lost weight with methanol, shake well, filter, and tak...
Embodiment 3
[0122] Based on Example 1, this example investigates the type of extraction solvent, extraction method, extraction time, and material ratio in the preparation of the test solution, and further illustrates with this technical solution.
[0123] First, the extraction solvent inspection
[0124] Take six parts of the powder to be tested, pass through No. 2 sieve, weigh 0.5g, put them in a stoppered conical flask, add 10mL of petroleum ether, 10mL of dichloromethane, 10mL of ethyl acetate, 10mL of water, 10mL of methanol, and ethanol. 10mL, weighed, ultrasonically treated (power 600W, frequency 40kHz) for 30min, let cool, weighed again, used the corresponding solvent to make up the lost weight, shake well, filter, and take the subsequent filtrate, the results are as follows figure 1 shown.
[0125] The results show that when the extraction solvent is methanol, the information of the chromatographic peaks is large and the resolution is good.
[0126] Second, the extraction method...
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