Method and apparatus for providing an isolated single cell

A single-cell, cell-based technology that can be used in chemical instruments and methods, biochemical equipment and methods, laboratory containers, etc., and can solve problems such as reducing image quality around the edge of droplets
CN112672825AActive Publication Date: 2021-04-16埃德蒙沃尔什
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
埃德蒙沃尔什
Publication Date
2021-04-16

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Abstract

Methods and apparatus for providing an isolated single cell are provided. In one disclosed arrangement, a test body of liquid is formed on a substrate surface. A contact angle between the test body of liquid and the substrate surface is lower than an equilibrium contact angle. An optical image of the test body of liquid is analysed to determine whether one and only one cell is present in the test body of liquid.
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Description

technical field

[0001] The present invention relates to methods and apparatus for providing isolated single cells, eg for monoclonal cell culture. Background technique

[0002] A wide range of applications involving monoclonal cell culture requires the generation of cell colonies from single cells with high reliability. These applications include, for example, the production of therapeutic monoclonal antibodies, stem cell therapy and gene editing. On well plates, this is challenging and time-consuming, and often impossible due to the so-called "edge effect" in which the solid walls of conventional microtiter plates interfere with the assays used for detection. Optical measurement of the presence of cells. Microplates (or microtiter plates / microwell plates) are widely used in liquid handling; each plate is essentially an array of microscopic test tubes. Plates have acceptable standard dimensions (127.76 x 85.48 x 14.22 mm); those with 96, 384, and 1536 wells / plate are comm...

Claims

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