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Method and apparatus for providing an isolated single cell

A single-cell, cell-based technology that can be used in chemical instruments and methods, biochemical equipment and methods, laboratory containers, etc., and can solve problems such as reducing image quality around the edge of droplets

Active Publication Date: 2021-04-16
埃德蒙沃尔什
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The curvature of the upper interface of each droplet degrades the image quality around the droplet edge

Method used

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  • Method and apparatus for providing an isolated single cell
  • Method and apparatus for providing an isolated single cell
  • Method and apparatus for providing an isolated single cell

Examples

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Embodiment Construction

[0036] As described in the Background section of the specification, edge effects can interfere with reliable determination of the presence or absence of single cells in a well of a well plate. exist figure 1 The problem is illustrated in the optical image of , where the presence of a wall makes cells adjacent to the wall optically unclear. In this example, cells can only be identified by using expensive optics and fluorescent or other cell markers. Even with expensive optics and labels, the presence of walls makes cell identification less reliable and potentially more time consuming. This can be done by passing figure 1 image with figure 2 with image 3 images for comparison to understand the size of the edge effects, figure 2 with image 3 respectively shows how single cells and adherent cells are more easily identified when solid walls are replaced by liquid walls.

[0037] Embodiments of the present disclosure provide methods and apparatus that allow the verifiable...

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Abstract

Methods and apparatus for providing an isolated single cell are provided. In one disclosed arrangement, a test body of liquid is formed on a substrate surface. A contact angle between the test body of liquid and the substrate surface is lower than an equilibrium contact angle. An optical image of the test body of liquid is analysed to determine whether one and only one cell is present in the test body of liquid.

Description

technical field [0001] The present invention relates to methods and apparatus for providing isolated single cells, eg for monoclonal cell culture. Background technique [0002] A wide range of applications involving monoclonal cell culture requires the generation of cell colonies from single cells with high reliability. These applications include, for example, the production of therapeutic monoclonal antibodies, stem cell therapy and gene editing. On well plates, this is challenging and time-consuming, and often impossible due to the so-called "edge effect" in which the solid walls of conventional microtiter plates interfere with the assays used for detection. Optical measurement of the presence of cells. Microplates (or microtiter plates / microwell plates) are widely used in liquid handling; each plate is essentially an array of microscopic test tubes. Plates have acceptable standard dimensions (127.76 x 85.48 x 14.22 mm); those with 96, 384, and 1536 wells / plate are comm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00C12Q1/24
CPCB01L3/50857B01L3/5085B01L3/5088B01L3/52B01L2200/10B01L2300/025B01L2300/161B01L2300/168B01L3/5025B01L2200/0668B01L2300/0609B01L2300/0819
Inventor 埃德蒙·沃尔什
Owner 埃德蒙沃尔什
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