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A method for extracting functional active substances of Tianshan snow lotus cells and its application

A technology of functional activity and cells, which is applied in the field of extracting functional active substances of Snow Lotus cells, can solve the problems of inactivation or decomposition of functional active ingredients of Snow Lotus, excess organic solvent residues, and difficult removal of organic solvents, etc., to improve industrial production efficiency , high extraction efficiency and simple operation

Active Publication Date: 2022-07-12
DALIAN PRACTICAL BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In the prior art, organic solvents are mostly used for the extraction of functional active ingredients. After the extraction is completed, some organic solvents are difficult to remove, resulting in excessive organic solvent residues. Moreover, the active ingredients of Snow Lotus Tianshan are all easily oxidative and decomposed components, and the process of evaporating the organic solvent is also difficult. It will lead to the partial inactivation or decomposition of the functional active ingredients of Saussurea tianshanensis

Method used

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  • A method for extracting functional active substances of Tianshan snow lotus cells and its application
  • A method for extracting functional active substances of Tianshan snow lotus cells and its application

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preparation example Construction

[0044] Preparation of reference solution: Precisely weigh 10 mg of rutin reference substance dried to constant weight at 120 °C, put it in a 50 mL volumetric flask, add an appropriate amount of methanol, heat in a water bath to dissolve, let cool, add methanol to the mark, and shake well. That is to obtain (the reference substance solution containing 0.2 mg of anhydrous rutin in each mL);

[0045] Preparation of standard curve: Precisely measure 1mL, 2mL, 3mL, 4mL, 5mL, and 6mL of the reference solution, put them in 25mL volumetric flasks, add water to 6mL each, add 1mL of 5% sodium nitrite solution, shake well, and leave for 6min. Add 10% aluminum nitrate 1mL, shake well, leave for 6min, add 10mL sodium hydroxide test solution, add water to the mark, shake well, leave for 15min, take the corresponding reagent without rutin as the blank, measure the absorbance at 500nm, take The absorbance is the ordinate, the concentration is the abscissa, and the standard curve is drawn;

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Embodiment 1

[0051] A. Take fresh Tianshan snow lotus cells and wash the snow lotus cells twice with 5 times the amount of pure water;

[0052] B. After washing the snow lotus cells, put them into a beaker, add 1 times the amount of pure water, and homogenize them with a homogenizer. The rotation speed of the homogenization method is 18000rpm, the homogenization time is 2min / time, and the homogenization is repeated 3 times:

[0053] C. The homogenized snow lotus cell water is filtered with 10 μm, 2 μm and 0.45 μm filter membranes in turn, and finally all passes through a 0.45um filter membrane to obtain the Tianshan snow lotus cell water extract, which is packaged and stored at -5°C.

[0054] It was determined that the extract contained 0.12% of total flavonoids, 0.011% of chlorogenic acid, 0.001% of syringin, 0.013% of 1,5-dicaffeoylquinic acid, and IC of DPPH free radical scavenging experiment 50 = 10.2 μl / mL, IC of ABTS free radical scavenging experiment 50 = 20.3 μL / mL.

Embodiment 2

[0056] A. Take fresh Tianshan snow lotus cells and wash the snow lotus cells 4 times with 3 times the amount of pure water;

[0057] B. After washing the snow lotus cells, add 2 times the amount of pure water, put them in a -20 ℃ refrigerator to freeze, and after the snow lotus cells are completely frozen, thaw and repeat three times;

[0058] C. The thawed snow lotus cells were filtered with 10 μm, 2 μm, and 0.45 μm filters in sequence, and finally all passed through a 0.45um filter to obtain the Tianshan snow lotus cell water extract, which was packaged and stored at -5°C.

[0059] It was determined that the extract contained 0.06% of total flavonoids, 0.006% of chlorogenic acid, 0.001% of syringin, 0.007% of 1,5-dicaffeoylquinic acid, and IC of DPPH free radical scavenging experiment 50 = 24.2 μl / mL, IC of ABTS free radical scavenging experiment 50 = 38.3 μL / mL.

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Abstract

The invention discloses a method for extracting functional active substances of Tianshan snow lotus cells and application thereof, and belongs to the technical field of extraction of functional active ingredients of plants. The method for extracting the functional active substances of Tianshan snow lotus cells in the present invention includes the following steps: taking fresh Tianshan snow lotus cells, washing with pure water, and vacuuming the surface water; adding pure water to the washed Tianshan snow lotus cells, and breaking the wall to obtain an extract ; Use filter membranes with different pore sizes in turn to filter the extract to obtain a filtrate, which is stored in separate packages. The extraction method disclosed by the invention has the advantages of simple operation, high extraction efficiency, high yield of active components, and improved industrial production efficiency.

Description

technical field [0001] The invention relates to the technical field of extraction of plant functional active components, in particular to a method for extracting functional active substances of Tianshan snow lotus cells and application thereof. Background technique [0002] Tianshan snow lotus is a rare and precious Chinese herbal medicine commonly used by folks in the alpine regions of western my country. It is a plant of the Asteraceae family. Mountains, the Pamirs and the Altai Mountains. Snow lotus has a long history of application, especially for medicinal purposes. It mainly contains flavonoids, sesquiterpene lactones, coumarin, organic acids, polysaccharides, etc. It has antioxidant, anti-rheumatic, analgesic, cardiovascular system regulation, anti-cancer and other biological activities . Due to indiscriminate mining in recent years, the number of wild Tianshan snow lotus has been greatly reduced, and its use has been restricted. [0003] Tianshan snow lotus cultiva...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/99A61Q19/00A61Q17/04A61Q19/08A23L33/105
Inventor 刘汉石金银兵黄利
Owner DALIAN PRACTICAL BIOTECH
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