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A kind of polypeptide fragment d and its application

A drug and inflammatory bowel disease technology, applied in the field of polypeptide fragment D and its application, can solve the problems of reducing the colonic histopathological score of IBD mice, easy to produce immunogenicity, and difficult to become a drug, so as to reduce the colonic histopathological Scoring, improvement of colonic pathological morphology, effect of small molecular weight

Active Publication Date: 2022-07-26
SHANGHAI LONGXIN BIOMEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the shortcomings of MIMP, which has an improving effect on inflammatory bowel disease in the prior art, which is prone to immunogenicity and difficult to become a drug, the purpose of the present invention is to provide a polypeptide fragment D (abbreviated as MP-D) and its application. The polypeptide fragment D of the present invention can significantly improve the colonic pathological morphology of IBD mice, reduce the colonic histopathological score and colonic interferon-γ expression level of IBD mice

Method used

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  • A kind of polypeptide fragment d and its application
  • A kind of polypeptide fragment d and its application
  • A kind of polypeptide fragment d and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Experiment on the effect of MP-D intervention on DSS-induced inflammatory bowel disease in mice

[0030] The sequence of the polypeptide fragment D (MP-D) used in this example is the amino acid sequence shown in SEQ ID No. 1, when the 9th amino acid Xaa is Gln, the 20th amino acid Xaa is Arg, and the 25th amino acid Xaa is Asn The sequence of THTVGSYFQVQNGYVGAFSRALGNNEYAMNS.

[0031] 1. Experimental method

[0032] 1.1 Establishment of a mouse model of acute inflammatory bowel disease

[0033] When a certain concentration of DSS solution is administered to mice, an acute inflammatory bowel disease model characterized by diarrhea, blood in the stool, ulcers, and granulocyte infiltration can be induced. The experimental grouping followed the principle of randomization, and the mice were randomly divided into stratified groups according to the weight of the mice. Forty healthy male C57BL6 mice were divided into four groups of 10 mice each:

[0034] Blank con...

Embodiment 2

[0060] The reagents, materials, equipment, and experimental methods used in this example are all the same as those in Example 1, except that the sequence of the polypeptide fragment D (MP-D) used in this example is the amino acid shown in SEQ ID No.1 The 9th amino acid Xaa of the sequence is Tyr, the 20th amino acid Xaa is Glu, and the 25th amino acid Xaa is the sequence when Thr is THTVGSYFYVQNGYVGAFSEALGNTEYAMNS.

Embodiment 3

[0062] The reagents, materials, equipment, and experimental methods used in this example are all the same as those in Example 1, except that the sequence of the polypeptide fragment D (MP-D) used in this example is the amino acid shown in SEQ ID No.1 The 9th amino acid Xaa of the sequence is Gly, the 20th amino acid Xaa is Ser, and the 25th amino acid Xaa is Pro, namely THTVGSYFGVQNGYVGAFSSALGNPEYAMNS.

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Abstract

The invention belongs to the technical field of biomedicine, and specifically relates to a polypeptide fragment D and an application thereof. The polypeptide fragment D has the amino acid sequence shown in SEQ ID No. 1, wherein the 9th amino acid Xaa is Tyr, Val, Gly, Ser, GIn or absent, amino acid Xaa at position 20 is Ser, GIn, Glu, Tyr, Arg or absent, and amino acid Xaa at position 25 is Asn, Thr, Ser, Pro, Leu or absent. MP-D significantly improved the colon pathological morphology of the IBD mouse model, decreased the colonic histopathological score and the expression of colonic interferon-γ, and had the ability to interfere with the occurrence of inflammatory bowel disease in mice.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to a polypeptide fragment D and its application. Background technique [0002] Inflammatory bowel disease (IBD) is an idiopathic chronic intestinal inflammatory disease. The lesions are mainly located in the colorectal segment, and involve the mucosa and muscularis mucosae. The severe ones include liver and gallbladder, muscle skin and coagulation. In terms of complications, 20-30% of patients with recurrent disease may be transformed into colorectal cancer, which is a very serious intestinal inflammatory disease, which can be divided into ulcerative colitis (UC) and Crohn's disease. (Crohn's disease, CD) two categories. At present, it is believed that IBD is an intestinal inflammatory reaction caused by abnormal innate immunity and acquired immunity of the intestinal mucosa under the interaction of multiple factors such as environment, heredity, infection and immuni...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/335A61K38/16A61P1/00A61P1/04A61P29/00A23L33/18
CPCA61K38/16A61P1/00A61P1/04A61P29/00C07K14/335A61K38/00A23L33/18C07K14/4702
Inventor 张彩华常英
Owner SHANGHAI LONGXIN BIOMEDICAL TECH CO LTD
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