Composition capable of regulating micro-ecological balance of skin and application of composition

A technology of ecological balance and composition, which is applied in skin care preparations, medical preparations containing active ingredients, and cosmetic preparations, etc., can solve problems such as hindering the wound healing process, and achieve the improvement of human skin barrier and repair of sensitive skin. muscle effect

Pending Publication Date: 2021-04-30
曾万祥
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although the mechanism of action of the skin microbiome on wound healing has not been fully elucidated and is still controversial, earlier studies have suggested that the skin microbiota can lead to changes in the composition and overall virulence of the wound microbiome through the formation of biofilms, thereby hindering wound healing. wound healing process

Method used

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  • Composition capable of regulating micro-ecological balance of skin and application of composition
  • Composition capable of regulating micro-ecological balance of skin and application of composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A composition for regulating skin micro-ecological balance, prepared according to the following method:

[0037] (1) Take each raw material according to Table 1, and pass through a 40-mesh sieve after air-drying, mixing and pulverizing;

[0038] (2) Prepare the seed liquids of Bacillus subtilis ATCC6633 and Lactobacillus acidophilus ATCC4356 respectively, the concentration of each seed liquid bacterial liquid is 1 billion CFU / mL, mix according to the weight of Table 1, make mixed bacterial liquid; Then inoculate and mix Bacterial liquid; put into a fermentation bag, seal it, and cultivate it at a constant temperature of 30°C for 10 days;

[0039] (3) Sterilize the fermented material, transfer it to the chromatographic column, pack the column, and compact it; then use 50% volume concentration of butanediol aqueous solution to elute, and the elution flow rate is controlled at 0.5BV / H;

[0040] (4) Collect 1BV of the eluate, centrifuge, take the supernatant, freeze and st...

Embodiment 2

[0044] Measure the minimum inhibitory concentration (MIC value) of the 1#-8# composition in Table 1 of Example 1 by plate coating method (to mold, yeast) and dilution culture method (to bacterium), and the results are shown in Table 2.

[0045] Table 2 The MIC value (ppm) of each test group

[0046] test group Lactobacillus Bifidobacteria Staphylococcus aureus Candida albicans 1# 15000 15000 600 1000 2# 20000 15000 200 450 3# 10000 10000 550 800 4# 60000 45000 3000 5000 5# 50000 60000 1000 6000 6# 80000 40000 2000 7000 7# 90000 35000 5000 5500 8# 65000 85000 6500 4500

[0047] As can be seen from Table 2, the test group 1#-3# is compared with the test group 4#-6#, and the composition compounded with Gentiana yunnanensis, chicory, and Fangji is harmful to the skin (Staphylococcus aureus, Candida albicans bacteria) was significantly lower than when used alone, while the inhibition of skin probi...

Embodiment 3

[0049] Volunteers were selected according to the subject selection criteria recorded in the "Safety and Technical Specifications for Cosmetics" (2015 Edition) human skin patch test, and divided into 8 groups, 30 person-times in each group. Select a standard spot tester and put about 0.020-0.025 g of the composition for adjusting the skin micro-ecological balance prepared in Example 1 Test Group 1-8 into the small chamber of the spot tester with a closed patch test method. Stick it on the subject's arm for internal testing, remove the spot tester after 24 hours, and observe the skin reaction at 0.5 hour, 24 hours, and 48 hours. Patch Test Skin Reaction Grading Standards” to record the results. When the number of grade 1 skin adverse reactions among the 30 subjects is more than 3, or the number of grade 2 skin adverse reactions is more than 1, or any one case of grade 3 or above skin adverse reactions occurs, it is judged The test substance has adverse skin reactions to humans....

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Abstract

The invention discloses a composition capable of regulating micro-ecological balance of skin and the application of the composition. The composition capable of regulating the micro-ecological balance of the skin is prepared from gentiana riparia, cichorium intybus and stephania tetrandra through a biological fermentation technology and a column chromatography separation technology. Experiments prove that the cosmetics added with the composition can obviously improve the human skin barrier and achieve the effect of repairing sensitive skin. The compound can be used as an active additive to be applied to cosmetics, and the cosmetics with the function of adjusting micro-ecological balance are prepared.

Description

technical field [0001] The invention relates to the field of cosmetics, in particular to a composition capable of regulating skin micro-ecological balance and its application. Background technique [0002] The skin microbiome is a dynamically variable and complex ecosystem, so it is also called the skin microecology. According to different classification methods, it can be divided into transient microorganisms and resident microorganisms; symbiotic microorganisms and pathogenic microorganisms, as well as conditional pathogenic microorganisms under certain circumstances. [0003] Transient microorganisms generally refer to microorganisms that only stay on the skin for a few hours or days and originate from the external environment. Resident microorganisms usually refer to a relatively fixed group of microorganisms that have the ability to self-recover after being affected by external factors (cleaning and exposure to air pollution, etc.). Resident microorganisms are general...

Claims

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Application Information

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IPC IPC(8): A61K8/9789A61Q19/00
CPCA61K8/9789A61Q19/00A61Q19/005A61K2800/85A61K2800/84A61K2800/5922Y02A50/30
Inventor 曾万祥
Owner 曾万祥
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