Multifunctional nano template based on aggregation-induced emission as well as preparation method and application of multifunctional nano template

An aggregation-induced luminescence, multi-functional technology, applied in the field of multi-functional nano-templates and their preparation, can solve the problems of impact, limited tissue penetration depth, etc., to increase tissue penetration, enhance ROS generation, and achieve photodiagnostic effects. Effect

Active Publication Date: 2021-05-28
SHENZHEN UNIV
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Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a multifunctional nano-template based on aggregation-induced luminescence and its preparation method and application in view of the above-mentioned defects of the prior art. It is reported that the PDT effect of AIE molecules is easily affected by the endogenous GSH of tumor cells, and the tissue penetration depth is limited.

Method used

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  • Multifunctional nano template based on aggregation-induced emission as well as preparation method and application of multifunctional nano template
  • Multifunctional nano template based on aggregation-induced emission as well as preparation method and application of multifunctional nano template
  • Multifunctional nano template based on aggregation-induced emission as well as preparation method and application of multifunctional nano template

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Embodiment 1

[0062] in N 2 Under protection, 0.415g MeOTT and 0.175g 1,3-indandione were added to a mixed solution of 10mL dry ethanol and 10mL chloroform, heated to 45°C in a 50mL round bottom flask and stirred at reflux for 12h. After the reaction was complete, the solvent was distilled off under reduced pressure to obtain a crude product. Using ether / dichloromethane (1:1) as an eluent, the obtained crude product was separated and purified by silica gel column chromatography to obtain 0.33 g of red solid product MeOTTI. Yield 60%.

Embodiment 2

[0064] 1mg MeOTTI molecular solid powder and 15mg DSPE-PEG 2000 -SH (commercial) was added to 1 mL THF, sonicated for 5 minutes and allowed to stand for 30 minutes. The above mixed solution was quickly injected into 9 mL of water, followed by ultrasonication with an ultrasonic probe for 2 minutes. The mixed solution was put into a dialysis bag (MWCO 8000-14000Da) for dialysis for 24 hours, and the water was changed every 4 hours. After centrifugal concentration, MeOTTI@DSPE-PEG nanoparticles (MeOTTI NPs) were finally obtained.

Embodiment 3

[0066] 1mg MeOTTI molecular solid powder, 15mg DSPE-PEG 2000 -SH (commercial) and 6 μL NaYF 4 :Yb 3+ ,Er 3+ (5 mg / mL in THF, commercially available) was added to 1 mL THF, sonicated for 5 minutes and allowed to stand for 30 minutes. The above mixed solution was quickly injected into 9 mL of water, followed by ultrasonication with an ultrasonic probe for 2 minutes. Put the mixed solution into a dialysis bag (MWCO8000-14000Da) and dialyze for 24 hours, changing the water every 4 hours. MeOTTI,UCNPs@DSPE-PEG nanoparticles (MUNPs) were finally obtained by centrifugal concentration.

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Abstract

The invention discloses a multifunctional nano template based on aggregation-induced emission and a preparation method and application thereof, and the preparation method comprises the following steps: carrying out reflux stirring on a mixed solution of dimethoxytriphenylamine thiophene and 1,3-indanedione to obtain MeOTTI; carrying out self-assembly on MeOTTI, DSPE-PEG2000-SH and NaYF4:Yb<3+> and Er<3+> (upconversion nanoparticles and UCNPs), and carrying out surface oxidation by using KMnO4, so as to obtain MeOTTI,UCNPs-coated DSPE-PEG-coated MnO2 nanoparticles. According to the nano template prepared by the method, on the basis of an I-type photodynamic photosensitizer with AIE property, endogenous glutathione is consumed by utilizing a MnO2 shell to retain active oxygen, and up-conversion nanoparticles are introduced to increase the tissue penetrability of light, so that a great-depth light diagnosis and treatment effect is realized.

Description

technical field [0001] The invention relates to the technical field of biomaterials, in particular to a multifunctional nano-template based on aggregation-induced luminescence and its preparation method and application. Background technique [0002] Phototherapeutics has been proven to be an effective strategy for personalized and precise cancer treatment due to its advantages of non-invasiveness and photocontrollability. In recent years, the development of phototherapeutic agents has received extensive attention, and small organic fluorophores with photosensitive properties stand out due to their good biocompatibility and biodegradability, as well as easy-to-tailor structure and optical properties. However, traditional fluorophores with planar conformation have defects such as aggregation-caused quenching (ACQ), small Stokes shift, and poor photostability due to their inherent defects. limits its practical application. In 2001, the team of Academician Tang Benzhong propos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/85C09K11/06C09K11/02C07D333/22A61K41/00A61K49/00A61K49/18A61P35/00
CPCC09K11/7773C09K11/06C09K11/02C07D333/22A61K41/0057A61K49/0002A61K49/186A61K49/0054A61K49/0021A61P35/00C09K2211/1007C09K2211/1014C09K2211/1092C09K2211/1011Y02E10/549
Inventor 王东王媛玮李有梅张志军唐本忠
Owner SHENZHEN UNIV
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