Method for preparing xylooligosaccharide by combining lactic acid with xylanase hydrolysis

A technology of xylooligosaccharide and xylanase, which is applied in the field of biomass degradation and conversion, can solve the problems of low selectivity of xylooligosaccharide and insufficient hydrolysis of raw material xylan, so as to overcome many inhibitors and save The effect of high cost and purity

Pending Publication Date: 2021-06-01
NANJING FORESTRY UNIV
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Problems solved by technology

[0003] It can be seen that the methods for preparing xylooligosaccharides in the prior art generally have

Method used

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  • Method for preparing xylooligosaccharide by combining lactic acid with xylanase hydrolysis

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[0023] The invention provides a preparation method of xylooligosaccharides, comprising the following steps: mixing agricultural and forestry wastes with a lactic acid solution with a volume concentration of 0-10%, performing a catalytic reaction, and filtering to obtain a treatment liquid containing xylooligosaccharides and filter residue; mixing the filter residue with a xylanase solution for enzymolysis to obtain an enzymolysis solution containing xylooligosaccharides.

[0024] The present invention is not particularly limited to the type of agricultural and forestry wastes, and any agricultural and forestry wastes containing xylan-type hemicellulose known in the art can be used, preferably agricultural and forestry wastes containing a large amount of xylan-type hemicellulose, such as Corn stalks, wheat stalks, corn cobs, etc. In some embodiments of the present invention, corn cobs are selected. In the present invention, it is preferable to grind the agricultural and forestr...

Embodiment 1

[0032] Lactic acid catalysis: Accurately weigh corn cobs with an absolute dry weight of 1g, add a lactic acid solution with a volume concentration of 2% according to a solid-liquid ratio of 1g:10ml, heat at 160°C for 40min, cool, and perform solid-liquid separation. A treatment solution containing xylo-oligosaccharides is obtained; the filter residue is washed with deionized water until neutral to obtain a solid residue, which is then air-dried naturally.

[0033] Enzymatic hydrolysis of xylan: Take the filter residue with an absolute dry weight of 0.06g in a 10ml centrifuge tube, add 36U of xylanase, adjust the pH value to 4.8 with sodium citrate buffer, shake well and place it in a constant temperature shaker at 50°C After 24 hours of enzymolysis, boil in a boiling water bath for 10 minutes to inactivate the enzyme, cool to room temperature, centrifuge, and obtain an enzymolysis solution containing xylooligosaccharides from the supernatant.

[0034]Use high-performance liqui...

Embodiment 2

[0042] The difference from Example 1 is that the corncobs are not subjected to lactic acid catalytic treatment, but are directly subjected to xylan enzymatic hydrolysis, and the rest of the enzymatic hydrolysis conditions are the same as in Example 1.

[0043] As a result, the yields of xylobiose, xylotriose, xylotetraose, xylopentaose and xylohexaose in the enzymolysis solution were 11.1%, 1.2%, 1.6%, 0.4% and 0.2%, respectively. The yield of xylose was 14.5%.

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Abstract

The invention provides a method for preparing xylooligosaccharide by combining lactic acid with xylanase hydrolysis, and belongs to the technical field of biomass degradation and conversion. The method comprises the following steps: mixing forestry and agricultural residues with a lactic acid solution with the volume concentration of 0-10%, carrying out a catalytic reaction, filtering to obtain a treatment solution containing xylooligosaccharide and filter residues, mixing the filter residues with a xylanase solution, and carrying out enzymolysis to obtain an enzymatic hydrolysate containing xylooligosaccharide. According to the method disclosed by the invention, a dilute acid is combined with an enzyme hydrolysis method, so that xylan in treating fluid and solid residues after catalysis of agricultural and forestry wastes can be fully degraded into xylooligosaccharide, and the yield of xylooligosaccharide can reach 70%.

Description

technical field [0001] The invention belongs to the technical field of biomass degradation and transformation, and in particular relates to a method for preparing xylooligosaccharides by hydrolyzing lactic acid combined with xylanase. Background technique [0002] my country produces a large amount of agricultural and forestry waste every year, for example, there are about 10 million tons of corn cobs alone. A variety of agricultural and forestry wastes contain a large amount of xylan-type hemicellulose, such as wheat straw, corn straw, and corn cobs. Corncob contains about 35% hemicellulose, 35% cellulose and 15% lignin, and the main component of hemicellulose is xylan, which is one of the best raw materials for producing xylooligosaccharides. At present, the commonly used preparation methods of xylooligosaccharides include enzymatic hydrolysis, alkali method, autohydrolysis method and acid method. For example, Yoon et al. (LWT-Food Science and Technology, 2006, 39(4): 38...

Claims

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Application Information

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IPC IPC(8): C12P19/00C12P19/04C12P19/12C12P19/14
CPCC12P19/00C12P19/04C12P19/12C12P19/14
Inventor 张军华杨倩倩徐勇应文俊文沛瑶
Owner NANJING FORESTRY UNIV
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