Method for regulating and controlling proliferation and differentiation of inner ear stem cells by Rps14 and Foxg1 and application of method

Abstract

The invention relates to the technical field of biology, in particular to a method for regulating and controlling proliferation and differentiation of inner ear stem cells by Rps14 and Foxg1 and application of the method. The method comprises the following steps: firstly, regulating and controlling proliferation and differentiation of Lgr5+ inner ear stem cells by an Rps14 gene: 1, culturing OC-1 cells; 2, knocking out the Rps14 gene from the siRNA; 3, carrying out isolated culture of Lgr5+ progenitor cells and Rps14 gene knockout; and 4, carrying out the biological effect of the Rps14 gene knockout Lgr5+ progenitor cells; and secondly, detecting the influence of knockout of the Foxg1 in the Lgr5+ progenitor cells on hair cell proliferation. According to the method, an Lgr5-EGFP-CreERT2 mouse and a Foxg1-floxp mouse are hybridized, and the Foxg1 is conditionally knocked out from the Lgr5+ progenitor cells, so that the fact that the cochlea of a newborn mouse can be induced to generate extra IPCs by knocking out the Foxg1 from the Lgr5+ progenitor cells is found. Rps14 genes are knocked out through OC-1 cells by utilizing different siRNAs, and the transfection effect is detected through RT-qPCR; and after the Lgr5+ inner ear stem cells are subjected to isolated culture and transfection, proliferation and differentiation are detected, and a balling differentiation experiment on the Lgr5+ stem cells shows that knock-down of the Rps14 gene can inhibit proliferation of the stem cells and has no influence on differentiation.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for regulating the proliferation and differentiation of inner ear stem cells by Rps14 and Foxg1 and its application. Background technique [0002] With the development and aggravation of adverse factors such as population aging, noise, virus infection, ototoxic drug abuse, and environmental pollution, the number of deaf and hearing-impaired people is gradually increasing. Deafness has become a global health problem affecting society, politics and economy, and sensorineural deafness accounts for about 63% of deaf patients. However, there is currently no clinically effective inner ear-targeted drug available for the treatment of sensorineural hearing loss. Since mammalian cochlear hair cells cannot regenerate, how to repair and regenerate hair cells after injury, so as to fundamentally treat sensorineural deafness, has become the focus of research in the field of hearing in r...

AI Technical Summary

Problems solved by technology

[0007] In order to solve the deficiencies mentioned in the above-mentioned background technology, the object of the present invention is to provide a method for regulating the proliferation and differentiation of inner ear stem cells by Rps14 and Foxg1 and its application, which solves the defect that the inner ear stem cells cannot be separated accurately and efficiently

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