Application of FoxM1 in diagnosis and treatment of intrauterine adhesion diseases

A technology of intrauterine adhesions and foxm1, applied in the field of biomedicine, can solve problems affecting endometrial fibrosis and other issues

Inactive Publication Date: 2021-06-04
NANJING DRUM TOWER HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So, whether there is a relationship between FoxM1 and intrauterine adhesions, and whether it affects endometrial fibrosis has not been reported so far

Method used

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  • Application of FoxM1 in diagnosis and treatment of intrauterine adhesion diseases
  • Application of FoxM1 in diagnosis and treatment of intrauterine adhesion diseases
  • Application of FoxM1 in diagnosis and treatment of intrauterine adhesion diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Expression of FoxM1 in endometrial tissues of patients with normal and severe intrauterine adhesions

[0018] 1. Materials, reagents, equipment

[0019] 1.1 Source of human endometrial tissue

[0020] Collect 24 cases of normal endometrial tissue and 24 cases of endometrial tissue from patients with severe intrauterine adhesions. When obtaining endometrial tissue, according to the hormone level, ensure that the endometrial tissue acquisition stage is unified into the late proliferation stage. All participants signed informed consent, which was approved by the Ethics Committee of Nanjing Drum Tower Hospital.

[0021] 1.2 Main reagents

[0022] TRNzol (Tiangen), chloroform, isopropanol, absolute ethanol, DEPC water, enzyme-free water, reverse transcription reagent (Yase), SYBRGreen (Yase), xylene, 95%, 80% and 75% ethanol , 3% hydrogen peroxide, citric acid repair solution, FoxM1 antibody (abcam), DAB chromogenic solution, hematoxylin, ammonia water, neutral ...

Embodiment 2

[0034] Example 2 TGFβ1 induces changes in FoxM1 expression in endometrial epithelial cell fibrosis

[0035] 1. Materials, reagents, equipment

[0036] 1.1 Main reagents

[0037] Type I collagenase (Sigma), hyaluronidase (Sigma), DNAase (Roche), PBS, epithelial cell culture medium (Gibco), FBS serum (Gibco), antibiotics, TGFβ1 (PeproTech), FoxM1 antibody (abcam), Electrophoresis solution, transfer solution, skimmed milk (bio-rad), TBST

[0038] 1.2 Main instruments

[0039] Filter screen, centrifuge, cell incubator, shaker, PVDF nitrocellulose membrane, double vertical electrophoresis tank, gel imaging analysis system

[0040] 1.3 Main method

[0041] 1.3.1 Isolation and culture of primary human endometrial epithelial cells

[0042] Clean the fresh intima tissue with antibiotic-containing PBS until no blood clots are visible to the naked eye, cut the tissue in a 60mm culture dish until there are no visible lumps, add the prepared tissue digestion solution, blow and mix wel...

Embodiment 3

[0047] Example 3 The diagnostic value of FoxM1 to intrauterine adhesions

[0048] 1. Statistical processing

[0049] Graphpad prism software was used for statistical analysis, and the data conforming to the normal distribution were linearly correlated with pearson correlation analysis, and P<0.05 was considered statistically significant.

[0050] 2. Results

[0051] There is a correlation between the expression level of FoxM1 in the endometrial tissue of patients with severe IUA and the thickness of the endometrium, r=0.5756, P=0.0079; the ROC curve of FoxM1 in the diagnosis of intrauterine adhesions shows that FoxM1 has diagnostic value for the diagnosis of intrauterine adhesions: The AUC was 0.8351, the standard error of the area was 0.06288, and the 95% CI of the area was 0.7130-0.9572 ( image 3 ).

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Abstract

The invention discloses application of FoxM1 in preparation of a reagent for diagnosing and treating intrauterine adhesion diseases. The invention relates to application of FoxM1 as a detection target in preparation of an intrauterine adhesion diagnostic reagent. The invention relates to application of FoxM1 in preparation of a medicine for treating diseases caused by uterine fibrosis. Experiments find that expression of FoxM1 in endometrial tissues of an intrauterine adhesion patient is remarkably reduced, particularly, reduction of epithelial cells is most remarkable, and the expression level of the FoxM1 has certain positive correlation with the endometrial thickness of the intrauterine adhesion patient. The ROC diagram shows that the FoxM1 has a good diagnostic value for intrauterine adhesion. An in-vitro result shows that the expression level of the FoxM1 is also obviously reduced in the TGF beta1-induced epithelial cell fibrosis. Therefore, FoxM1 can play an important role in detection and treatment of intrauterine adhesion, and can be used for clinical diagnosis and treatment of intrauterine adhesion.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to the application of a Forkhead family transcription factor FoxM1 in the diagnosis and treatment of intrauterine adhesions. Background technique [0002] Intrauterine adhesion (IUA) is the damage of the endometrial basal layer caused by various factors, and the functional repair of the endometrium is impaired, and the adhesion of the inner wall of the uterus results in partial or complete occlusion of the uterine cavity. The clinical manifestations are abnormal menstruation (reduced menstrual flow or amenorrhea), infertility or recurrent miscarriage, which is one of the most common causes of uterine infertility. The pathogenesis of IUA is unclear, and abortion, infection and iatrogenic injury are common causes. In recent years, with the increase of hysteroscopy and painless abortion, the incidence of IUA in my country is on the rise. Histologically, the endometriu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883G01N33/68A61K45/00A61P15/08
CPCA61K45/00A61P15/08C12Q1/6883C12Q2600/136C12Q2600/158G01N33/6893G01N2333/4703G01N2800/367
Inventor 胡娅莉赵光锋周振华李若天戴建武
Owner NANJING DRUM TOWER HOSPITAL
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