Cudrania tricuspidata tissue culture seedling subculture method
A technology of subculture and tissue culture seedlings, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems that Zhemu cannot be artificially propagated on a large scale, so as to reduce the risk of component uncertainty and facilitate control , the effect of high survival rate
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[0023] One, carry out subculture (method A) of Zhe wood tissue culture seedling as follows:
[0024] The selection of explant: select robust Zhemu biennial seedling, use the stem section of band axillary bud as explant;
[0025] Disinfection of explants: first rinse the explants with tap water for 5-8min, rinse the surface; cut the stem into 2-3cm segments, put them in a container and rinse them again; soak them in 70% alcohol for 1min, transfer Go to the ultra-clean workbench, then soak it with 3% sodium hypochlorite solution for 10 minutes, and finally soak it with 0.15% sterile citric acid water for 3 times, dry it and inoculate it on the starting medium.
[0026] Start-up culture: inoculate the treated explants on the start-up medium (medium ZS01) on the ultra-clean workbench, and insert one explant stem segment into each test tube. After 28 days of cultivation, the sterile vaccine can be obtained. Temperature: 25±3°C; light intensity: 1500±300lux; light culture 14h / dark...
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