Cudrania tricuspidata tissue culture seedling subculture method

A technology of subculture and tissue culture seedlings, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems that Zhemu cannot be artificially propagated on a large scale, so as to reduce the risk of component uncertainty and facilitate control , the effect of high survival rate

Pending Publication Date: 2021-06-11
苏州回文生物种业科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the invention is to solve the defect that Zhemu cannot be artificially propagated on a large scale in the prior art, utilize tissue culture technology to carry out the problem of Zhemu multiplication and multiplication, and rapidly propagate the Zhemu of excellent varieties

Method used

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  • Cudrania tricuspidata tissue culture seedling subculture method
  • Cudrania tricuspidata tissue culture seedling subculture method
  • Cudrania tricuspidata tissue culture seedling subculture method

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Embodiment Construction

[0023] One, carry out subculture (method A) of Zhe wood tissue culture seedling as follows:

[0024] The selection of explant: select robust Zhemu biennial seedling, use the stem section of band axillary bud as explant;

[0025] Disinfection of explants: first rinse the explants with tap water for 5-8min, rinse the surface; cut the stem into 2-3cm segments, put them in a container and rinse them again; soak them in 70% alcohol for 1min, transfer Go to the ultra-clean workbench, then soak it with 3% sodium hypochlorite solution for 10 minutes, and finally soak it with 0.15% sterile citric acid water for 3 times, dry it and inoculate it on the starting medium.

[0026] Start-up culture: inoculate the treated explants on the start-up medium (medium ZS01) on the ultra-clean workbench, and insert one explant stem segment into each test tube. After 28 days of cultivation, the sterile vaccine can be obtained. Temperature: 25±3°C; light intensity: 1500±300lux; light culture 14h / dark...

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Abstract

The invention belongs to the field of plant cultivation, relates to a plant tissue culture method, in particular to a cudrania tricuspidata tissue culture seedling subculture method, and belongs to the technical field of biology. The method comprises the following steps of 1) selecting explants: selecting robust cudrania tricuspidata seedlings, and taking stem segments with axillary buds as the explants; 2) disinfecting the explants: disinfecting the explants with a disinfectant, airing the disinfected explants, and inoculating the aired explants onto a starting culture medium; 3) performing starting culture: inoculating the treated explants onto the starting culture medium on an ultra-clean workbench, inoculating one explant stem segment into each test tube, and performing culture for 28 days to obtain aseptic seedlings; and 4) performing proliferation / subculture: taking the terminal buds, the base bud clusters and the stem segments of the aseptic seedlings for subculture and proliferation. According to the method, the proliferation coefficient can reach 1: (4-4.5), and the plant height is about 3.5 cm; and the survival rate and the qualification rate are higher than 98%.

Description

technical field [0001] The invention belongs to the field of plant cultivation, and relates to a method for plant tissue culture, in particular to a method for subculture of eucalyptus tissue culture seedlings, and belongs to the field of biotechnology. Background technique [0002] Cudrania tricuspidata (scientific name: Cudrania tricuspidata), also known as mulberry tree, belongs to the Moraceae plant. It is widely distributed in East China, Central South, Southwest to southern Hebei. It is a famous and precious wood in the history of our country, and it is recorded in most historical documents. Its wood is fine, smooth and heavy, hard material, high compressive strength, high corrosion resistance and abrasion resistance; bast fiber is a high-quality papermaking raw material; Used as medicine, it has the effects of expelling wind and dampness, promoting blood circulation and promoting menstruation, relieving cough and reducing phlegm, and can treat rheumatic joint pain, ja...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张文杰尹翠翠冷江明郭俊清宋斌
Owner 苏州回文生物种业科技有限公司
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