A kind of African swine fever virus rapid detection test strip and its detection method

Abstract

The invention discloses a test paper strip for rapid detection of African swine fever virus, which comprises a support plate. A sample pad, a reagent pad, a detection area and a water absorption pad are sequentially adhered on the surface of the support plate from a test end to a water absorption end. The usage method of described test strip: collect the sample to be tested and add phosphate buffer saline or water to mix; Drop into the sample pad of the test strip; Place it horizontally for 5-10 minutes, add chlorine dropwise at the detection line and the control line Copper chloride ethanol solution, observe the result. The test strip of the present invention can directly use pig whole blood and body fluid detection in the whole process of detecting African swine fever, and has the advantages of convenience, rapidity, economy, sensitivity and specificity, and is especially suitable for on-site detection of African swine fever virus, epidemic diseases, etc. It has practical application significance for scientific investigation and pig international trade quarantine.

Description

technical field [0001] The invention belongs to the technical fields of immune analysis and animal infection diagnosis, and specifically relates to a test strip for rapid detection of African swine fever virus and a detection method thereof. Background technique [0002] African swine fever is an acute, hemorrhagic and severe infectious disease caused by African swine fever virus (English name: African swine fever virus, referred to as ASFV) infecting domestic pigs and various wild boars (such as African wild boars, European wild boars, etc.). The World Organization for Animal Health lists it as a legally notifiable animal disease, and this disease is also a type of animal epidemic that my country focuses on preventing. It is characterized by a short course of disease, the most acute and acute infection mortality rate is as high as 100%, clinical manifestations are fever (up to 40-42 ℃), rapid heartbeat, dyspnea, partial cough, serous or mucous purulence in eyes and nose Se...

AI Technical Summary

Problems solved by technology

[0004] In order to solve the defect that most of the detection of African swine fever virus in the prior art can only rely on laboratory detection, the present invention provides a rapid detection test strip of African swine fever virus and its detection method, the purpose of which is to have a safer and more reliable , rapid advantages, solve the problem that other existing methods need special reagents and equipment when detecting ASFV, and the problem that needs to be tested in the laboratory

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