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A kind of inactivated vaccine of goose astrovirus and preparation method thereof

An astrovirus and inactivated vaccine technology, applied in the biological field, can solve the problems of large economic losses, little research on goose astrovirus vaccines, and no commercialization, and achieve good immune performance, prevention of virus infection, and strong virulence. Effect

Active Publication Date: 2022-06-28
NANYANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The lack of suitable proliferative cell lines in vitro has resulted in relatively few attenuated or inactivated vaccines that can prevent and control avian Astrovirus infection, and there are no commercial goose Astrovirus vaccine products
[0003] The research and development of a variety of vaccines have been reported in the prior art. For example, Rong Xuelu et al. have developed and verified the immune effect of the recombinant Capsid protein subunit vaccine of goose Astrovirus. The research results show that the immune effect of the recombinant Capsid protein subunit vaccine of goose Astrovirus is Good, can effectively prevent the infection of goose astrovirus; patent document CN110699331A reports a preparation method and application of a goose astrovirus attenuated virus, which adopts a method of combining high temperature treatment and low temperature incubation to attenuate the virus for the preparation of vaccines It can effectively prevent goose gout caused by goose astrovirus. However, there are still few researches on goose astrovirus vaccines. Based on the current spread of goose astrovirus in farms, the economic losses caused are increasing. Therefore, There is an urgent need to develop safe and effective vaccines, yolk antibodies and drugs for the prevention and treatment of goose Astrovirus, in order to effectively control the Goose Astrovirus epidemic and avoid economic losses in the breeding industry

Method used

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  • A kind of inactivated vaccine of goose astrovirus and preparation method thereof
  • A kind of inactivated vaccine of goose astrovirus and preparation method thereof
  • A kind of inactivated vaccine of goose astrovirus and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Isolation and identification of goose astrovirus

[0026] 1. Collection of disease materials

[0027] The diseased material samples were collected from the diseased farm in Nanyang, Henan Province. The diseased goose species included: Taizhou goose and Langde goose. The dead goslings showed joint swelling and white free urate deposition. The necropsy showed that white urate deposits were deposited in the heart, Liver and kidney and other parts, some cases also have urate deposition in the subcutaneous and gallbladder.

[0028] Referring to the method in Zhang Qingshui's paper (Zhang Qingshui. Isolation and identification of new renal pathogenic goose astroviruses and breeding of attenuated strains [D]. China Agricultural University, 2019.), the possible existence of the gosling samples was analyzed. Goose parvovirus (GPV), goose hemorrhagic polyoma virus (GHPV), duck reovirus (DRV), Tembusu virus (Tembusu) and goose astrovirus (GoAstV) and other viral genes ...

Embodiment 2

[0046] Example 2: Preparation of goose astrovirus inactivated vaccine

[0047] 1. Preparation of Inactivated Vaccines

[0048] Step 1, take the 15th-20th generation goose astrovirus N-GoAstV-190123 virus seed and dilute it with diluent, and dilute it to ELD 50 5×10 5.00 / mL, inoculate 8-10-day-old SPF goose embryos via the chorioallantoic membrane route, 0.2ml per embryo, and continue to incubate at 37°C after inoculation without turning the eggs;

[0049] Step 2: After inoculation of SPF goose embryos, light the eggs 3 times a day, discard the goose embryos that died before 24 hours, and take out the goose embryos that died after 24 hours at any time, until 72 hours, take out all the live embryos with the air chamber facing upwards. Stand upright and cool at 4°C for 12 hours;

[0050] Step 3: Take out the goose embryos that have been cooled for 12 hours. After surface disinfection, suck the goose embryo fluid. Mix the embryo fluid of every 20 goose embryos into a group. Wh...

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Abstract

The invention relates to an inactivated vaccine of goose astrovirus and a preparation method thereof. A goose astrovirus N-GoAstV-190123 strain isolated and obtained with strong virulence and good immune performance is used as an antigen to prepare the virus strain After immunization with an inactivated vaccine, it can produce higher neutralizing antibodies, and can achieve full protection for geese.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, mainly relates to the production of vaccines, and in particular relates to an inactivated goose astrovirus vaccine and a preparation method thereof. Background technique: [0002] In recent years, a fatal infectious disease with gout as the main symptom has broken out in goose farms in Shandong, Jiangsu, Anhui and other regions. Several scholars have reported related cases. Research has found that the pathogen causing the outbreak is Goose Astrovirus. Especially since 2017, goose goose disease has spread rapidly across the country, which has brought huge economic losses to my country's goose industry and seriously threatened the development of my country's goose industry. Goose astrovirus is a member of the avian astrovirus genus, which belongs to RNA virus. Goose astrovirus infection manifests as diarrhea, arthritis and growth disorders, and serious fatal diseases such as hepatitis, cholecystitis ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/12A61P31/14C12N7/00C12R1/93
CPCA61K39/12A61P31/14C12N7/00A61K2039/5252A61K2039/552C12N2770/12034C12N2770/12021
Inventor 姚伦广冀君刘阳坤许鑫胡小敏
Owner NANYANG NORMAL UNIV
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