A non-enzymatic electrochemical sensor for the simultaneous detection of glucose and uric acid

A sensor and electrochemical technology, applied in the field of non-enzymatic electrochemical sensors, can solve the problems of simultaneous detection of glucose and other problems, achieve excellent catalytic ability, wide application prospects, and overcome the effect of easy deactivation

Active Publication Date: 2021-09-28
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that this type of non-enzymatic uric acid electrochemical sensor can achieve simultaneous detection with ascorbic acid, dopamine, epinephrine, nitrite ions, etc., but cannot achieve simultaneous detection with glucose

Method used

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  • A non-enzymatic electrochemical sensor for the simultaneous detection of glucose and uric acid
  • A non-enzymatic electrochemical sensor for the simultaneous detection of glucose and uric acid
  • A non-enzymatic electrochemical sensor for the simultaneous detection of glucose and uric acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] (1) Ultrasonic the ITO conductive glass with a cleaning agent for 30 minutes, then clean it with distilled water, then use acetone, ethanol, and distilled water to sonicate for 5 minutes, then soak the ITO conductive glass in sodium hydroxide solution for 8 hours, and wash it with deionized water Blow dry after rinsing;

[0054] (2) 2.4×10 -2 mol / L sodium citrate solution and 2.4×10 -2 Take 0.5mL of each mol / L tetrachloroauric acid solution, set the volume to 50mL, slowly add 0.1mol / L sodium borohydride solution dropwise under magnetic stirring, stop the dropwise addition when the solution turns from colorless to pink, and prepare A gold nanoparticle solution with an average particle diameter of 158nm was obtained;

[0055] (3) Get the gold nanoparticle solution in step (2), 30% stock gel solution, 1.0mol / L 3-hydroxyaminomethane hydrochloric acid buffer (pH=8.8), 5% sodium lauryl sulfate solution, 5% ammonium persulfate solution and tetramethylethylenediamine solutio...

Embodiment 2

[0062] The preparation method of the sensor based on gold nanoparticle gradient polyacrylamide gel modification described in this embodiment is different from Example 1 in that: in step (3), gold nanoparticle solution, 30% stock gel solution , 2.0mol / L 3-hydroxyaminomethane hydrochloric acid buffer solution (pH=8.8), 15% sodium lauryl sulfate solution, 15% ammonium persulfate solution and tetramethylethylenediamine solution according to volume ratio 4.6: 2.7 : 2.5: 0.1: 0.1: 0.006 mixed to prepare gel solution I; in step (5), gold nanoparticle solution, 30% stock gel solution, 1.5mol / L 3-hydroxyaminomethane hydrochloric acid buffer (pH =6.8), 15% sodium lauryl sulfate solution, 15% ammonium persulfate solution and tetramethylethylenediamine solution are mixed according to volume ratio 4.1: 1: 0.75: 0.06: 0.06: 0.006 to obtain a gel solution Ⅱ; The sensor based on gradient polyacrylamide gel modification of gold nanoparticles was prepared.

[0063] The 30% reserve gel solution...

Embodiment 3

[0065] The preparation method of the sensor based on gold nanoparticle gradient polyacrylamide gel modification described in this embodiment is different from Example 1 in that: in step (3), gold nanoparticle solution, 30% stock gel solution , 1.5mol / L 3-hydroxyaminomethane hydrochloric acid buffer solution (pH=8.8), 10% sodium lauryl sulfate solution, 10% ammonium persulfate solution and tetramethylethylenediamine solution according to volume ratio 2.3:5 : 2.5: 0.1: 0.1: 0.004 mixed to prepare gel solution I; in step (5), gold nanoparticle solution, 30% stock gel solution, 1mol / L 3-hydroxyaminomethane hydrochloric acid buffer (pH = 6.8), 10% sodium lauryl sulfate solution, 10% ammonium persulfate solution and tetramethylethylenediamine solution are mixed according to volume ratio 1.4: 0.33: 0.25: 0.02: 0.02: 0.02 to obtain gel solution II ; A gold nanoparticle-based gradient polyacrylamide gel-modified sensor was prepared.

[0066] The 30% reserve gel solution was purchased ...

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Abstract

The invention discloses an electrochemical sensor capable of simultaneously detecting glucose and uric acid, which belongs to the technical field of detection. The invention makes the surface of the sensor electrode rich in -OH groups through the pretreatment of the sensor electrode, so as to improve the cleaning and activation ability of the electrode. Among the modified materials for sensor electrodes, gold nanoparticles have a large specific surface area, excellent electrical conductivity and catalytic ability, which can enhance the catalytic ability to glucose or uric acid, and the gradient polyacrylamide gel can realize the enrichment of the analyte glucose or uric acid The role of separation can not only realize the quantitative detection of a single component, but also realize the simultaneous detection of two components, which solves the technical problem that commercial sensor test paper cannot be used universally, provides more ideas for medical diagnostic methods, and has a wide range of applications. Application prospects.

Description

technical field [0001] The invention belongs to the technical field of electrochemical sensors, in particular to a non-enzyme electrochemical sensor that can be used to simultaneously detect glucose and uric acid. Background technique [0002] With the development of society and economy, people's living standards and dietary structure changes, the prevalence of diabetes and hyperuricemia is increasing year by year, and showing a younger trend, has become the world's largest and second largest metabolic chronic disease , and China is one of the countries with the largest number of patients and the highest incidence rate, which brings a certain burden and impact on social economy and personal life. [0003] Diabetes is a metabolic disease characterized by high blood sugar. Long-term high blood sugar can cause chronic damage and dysfunction of the eyes, kidneys, heart, blood vessels, and nerves. Therefore, as the only standard for clinically diagnosing diabetes, the detection ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/36G01N27/327G01N27/48
CPCG01N27/3278G01N27/36G01N27/48
Inventor 张帼威封顺石海珠陈诚张华菊
Owner SOUTHWEST JIAOTONG UNIV
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