Double-layer micro-fluidic chip and kit and method for detecting novel coronavirus

Abstract

The invention belongs to the technical field of virus detection, and relates to a double-layer micro-fluidic chip, a kit and a method for virus nucleic acid detection. The double-layer micro-fluidic chip comprises a sample adding layer and a detection layer, and the sample adding layer is provided with at least two sample adding ports; the detection layer is provided with a detection cavity and a sample outlet; the detection cavity is connected with the sample adding ports through a sample injection micro-channel; and the sample outlet is connected with the detection cavity through a sample outlet micro-channel. The kit provided by the invention comprises the double-layer micro-fluidic chip, a detection solution containing RdRP gene and E gene fluorescent probes, a nano material solution, a coronavirus standard solution and a cation solution. The closed double-layer micro-fluidic chip detection system is adopted, different articles or reagents are added from different sample adding ports, and pollution interference caused by external nucleic acid can be reduced. According to the kit, a nanotechnology and a microfluidic technology are combined, the detection result can be obtained within one hour, and the kit is high in specificity and sensitivity.

Description

technical field [0001] The invention belongs to the technical field of virus detection, and relates to a double-layer microfluidic chip, a kit and a method for virus nucleic acid detection. Background technique [0002] Coronaviruses are a large family of viruses known to cause colds as well as more serious illnesses such as Middle East Respiratory Syndrome (MERS) and Severe Acute Respiratory Syndrome (SARS). The novel coronavirus is a new strain of coronavirus that has never been found in humans before. The diagnosis of the new coronavirus needs to be tested by nucleic acid, and it can only be confirmed if it is positive. At present, the detection of nucleic acid by real-time fluorescent quantitative PCR method shows a high false negative rate, which will make many infected patients mistakenly believe that they have not yet been infected, thereby causing a wider range of human-to-human infection. Therefore, in order to better control the novel coronavirus epidemic, it is ...

AI Technical Summary

Problems solved by technology

[0004] Existing novel coronavirus detection kits have problems such as low detection accuracy, high false positive and false negative rates, and the detection process is time-consuming, which cannot meet the needs of rapid detection

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