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Fingerprint spectrum of isodon lanceolata and establishment method and application thereof

A technology of hairy tea leaves and fingerprints, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve high sensitivity, good separation and identification, and improve the effect of quality evaluation system

Pending Publication Date: 2021-06-25
HANGZHOU HUQINGYUTANG PHARM CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report on the fingerprint and its establishment method of the chrysanthemum in the prior art. Therefore, it is of great application significance to develop a fingerprint that can comprehensively evaluate the quality of the chrysanthemum.

Method used

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  • Fingerprint spectrum of isodon lanceolata and establishment method and application thereof
  • Fingerprint spectrum of isodon lanceolata and establishment method and application thereof
  • Fingerprint spectrum of isodon lanceolata and establishment method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0047] The establishment of embodiment 1 fingerprint

[0048] (1) Preparation of the test solution: take 1 g of the powder of the aboveground part of the basilisk, accurately weighed, accurately add 25 mL of 70% methanol aqueous solution containing 0.2% formic acid, weigh, ultrasonically treat for 30 minutes, let cool, and weigh To weigh, make up the lost weight with 70% methanol aqueous solution containing 0.2% formic acid, shake well, filter, and take the subsequent filtrate to obtain the product.

[0049] (2) Carry out sample injection analysis according to the following chromatographic conditions, and record the chromatogram.

[0050] Chromatographic conditions: Kromasil 100-5C18 chromatographic column (250mm×4.6mm, 5μm); methanol (A)-0.1% glacial acetic acid aqueous solution (B) gradient elution, the elution program is shown in Table 1, the running time is 60min; the volume flow rate is 1.0mL / min; detection wavelength 239nm; column temperature 30°C; injection volume 5 μ...

Embodiment 2

[0056] Embodiment 2 precision test

[0057] Get the above-ground part powder of the fragrant tea vegetable (my023) sample, prepare the test solution according to the method, and measure according to the above chromatographic conditions, continuously inject the sample for 6 times, and the obtained chromatogram is processed by the similarity software, and the similarity of the result is 0.999~1.000. Taking chromatographic peak 11 (Oridonin A) as the reference peak, the relative retention time RSD values ​​of the other 11 chromatographic peaks were between 0.02% and 0.28%, which indicated that the precision of the method was good.

Embodiment 3

[0058] Embodiment 3 repeatability test

[0059] Take 6 powders of the above-ground part of the same batch of chrysanthemum (my023) samples, prepare No. 1 to No. 6 test solutions according to the method, and respectively inject and measure according to the above chromatographic conditions, and the obtained chromatograms are input by similarity software. Processing, the result similarity is 0.999-1.000. Taking chromatographic peak 11 (oridonin A) as the reference peak, the relative retention time RSD values ​​of the other 11 chromatographic peaks were between 0.09% and 0.99%, indicating that the method had good repeatability.

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Abstract

The invention discloses a fingerprint spectrum of isodon lanceolata and an establishment method and application thereof, the method comprises preparation of a test sample and high performance liquid chromatography detection conditions, and the high performance liquid chromatography detection conditions are as follows: a chromatographic column is a Kromasil 100-5C18 chromatographic column (250mm*4.6mm, 5[mu]m); a mobile phase is a methanol -0.1% glacial acetic acid aqueous solution; a gradient elution mode is adopted: 0 min, 10 min, 26 min and 60 min, and methanol is 20%, 35%, 37% and 70%; the sample size is 3-10 [mu]L; and the detection wavelength is 239 nm, the flow velocity is 1 mL / min, and the column temperature is 20-35 DEG C. According to the isodon lanceolatus medicinal material fingerprint spectrum established by the invention, 12 common peaks are determined, through the abundant chromatographic peak information and the evaluation of the similarity of the common peaks, the overall evaluation of the quality of the medicinal material is facilitated, the uniformity and stability of the internal quality of the medicinal material are ensured, the quality of the isodon lanceolatus medicinal material can be effectively monitored, and the authenticity of the isodon lanceolatus medicinal material can be effectively identified.

Description

[0001] This divisional application is based on the divisional application of the original Chinese patent application with the application number 201711191092.6, the application date is November 24, 2017, and the invention title is "Fingerprint Spectrum of a Fragrant Tea Vegetable and Its Establishment Method and Application" Apply. technical field [0002] The invention relates to the technical field of fingerprints of traditional Chinese medicinal materials, in particular to a fingerprint of Chinese herbal tea and its establishment method and application. Background technique [0003] Rabdosia japonica (Burm.f.) Hara is a plant of the genus Latiabae, mainly produced in Jiangsu, Henan, southern Shanxi, southern Shaanxi, southern Gansu, and northern Sichuan. The folks are called Silonggan, Shansuzi, and Mengyisa (Henan). Studies have shown that tea leaves mainly contain diterpenoids, phenolic acids and flavonoids, which are mainly anti-tumor, anti-bacterial and anti-inflamma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/86
CPCG01N2030/027G01N30/8686
Inventor 陈炜陈碧莲韩俊琦毛思浩
Owner HANGZHOU HUQINGYUTANG PHARM CO LTD
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