Low-temperature preservation method of pathogenic strain

Abstract

The invention relates to a low-temperature preservation method of pathogenic bacteria, and belongs to the technical field of pathogenic bacteria preservation. The low-temperature preservation method of yersinia provided by the invention comprises the following steps: mixing a low-temperature protective agent with a bacterial suspension, uniformly oscillating, and preserving at-70 DEG C to-90 DEG C. The low-temperature protective agent comprises glycerol, sucrose, L-carnitine and a PBS (Phosphate Buffer Solution) buffer, and the molar ratio of glycerol to sucrose to L-carnitine is (2-9): (0.2-0.9): (0.5-2). In the low-temperature preservation method of the pathogenic bacteria, the single protective agent glycerol, sucrose and L-carnitine are mixed to serve as the composite protective agent, and the preservation effect is obviously superior to that of the single protective agent, so that the low-temperature protective agent provided by the invention has a relatively high survival rate on the low-temperature preserved yersinia pestis, and meanwhile, the low-temperature preservation method of the pathogenic strain provided by the invention not only can reduce the toxicity of the system, but also has a relatively good preservation effect, so that the yersinia pestis can be effectively preserved.

Description

technical field [0001] The invention belongs to the technical field of preservation of pathogenic bacteria, and in particular relates to a low-temperature preservation method of pathogenic bacteria. Background technique [0002] Microbial bacteria (virus) species preservation methods can be generally divided into four categories: subculture preservation method, dry preservation method, freeze-drying method and cryopreservation method. Cryopreservation is a reliable method for long-term preservation of most microorganisms, the morphological and physiological characteristics are more stable after cryopreservation, no infrastructure is required (except for -80°C refrigerator or liquid nitrogen (except -196°C)), so it is better to study in the laboratory It is the most commonly used method, which has a long storage time, a wide range of applications, and a low variation rate of biological information. [0003] Yersinia pestis (Yersinia pestis, referred to as Yersinia pestis) is...

AI Technical Summary

Problems solved by technology

At present, semi-solid preservation is widely used in the laboratory preservation of strains. This method has a short preservation time and requires periodic su

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