A method for collagen purification

A technology of collagen and purification methods, applied in peptide preparation methods, chemical instruments and methods, animal/human proteins, etc., can solve the problems of unsatisfactory collagen yield and purity, low risk, etc., and achieve improved yield and purity, increase the amount of precipitation, the effect of low cost

Active Publication Date: 2022-03-11
浙江崇山生物制品有限公司
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Problems solved by technology

[0009] Chinese patent application 201610893949.8 provides an efficient and simple collagen ultrafiltration purification method, which mainly includes the following steps: dilute the dissolved collagen solution; turn on the delivery pump, and the collagen solution is purified through the purification system. Purification can produce purified collagen. This purification method is safe, effective, low-risk, simple, energy-saving, high production efficiency, does not pollute the environment, and is suitable for industrial production. However, the yield and purity of the obtained collagen are low. can not meet the requirements

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  • A method for collagen purification

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Embodiment 1

[0050] Embodiment 1 A kind of collagen purification method

[0051] Specifically include the following steps:

[0052] (1) Take the crude collagen extract (210L, containing about 400g of collagen), dilute it with water, add acetic acid to adjust the pH value to 2.5, add diatomaceous earth (2.1kg) and biological flocculant ( 0.7kg), stirred for 30min and adsorbed, followed by plate and frame filtration to obtain mixed solution A;

[0053] (2) Add an equal volume to the mixed solution A and the volume ratio is 5:1, the mass fraction is 15% ammonium chloride and the mass fraction is 30% ammonium sulfate mixed solution to carry out salting out for 2h to obtain solid B, and the solid B After reconstitution, the obtained reconstituted solution is first filtered with a 5-micron filter membrane, the obtained filtrate a is then filtered with a 3-micron filter membrane, and the obtained filtrate b is then filtered with a 0.45-micron filter membrane to obtain a mixture C;

[0054] (3) ...

Embodiment 2

[0056] Embodiment 2 A kind of collagen purification method

[0057] Specifically include the following steps:

[0058] (1) Take the crude collagen extract (210L, containing about 400g of collagen), dilute it with water, add acetic acid to adjust the pH value to 3.5, add diatomaceous earth (8.4kg) and biological flocculant ( 1.4kg), plate and frame filtration was carried out after adsorption, and then 0.1 μm membrane filtration was carried out to obtain the mixed solution A;

[0059] (2) Add an equal volume of ammonium chloride with a volume ratio of 10:1 and a mixed solution of ammonium sulfate with a mass fraction of 25% and 40% ammonium sulfate in the mixed solution A for salting out to obtain a solid B, and redissolve the solid B Finally, the obtained complex solution is first filtered with a 5-micron filter membrane, the obtained filtrate a is then filtered with a 3-micron filter membrane, and the obtained filtrate b is then filtered with a 0.45-micron filter membrane to ...

Embodiment 3

[0062] Embodiment 3 A kind of collagen purification method

[0063] Specifically include the following steps:

[0064] (1) Take the crude collagen extract (210L, containing about 400g of collagen), dilute it with water, add acetic acid to adjust the pH value to 3.0, add diatomaceous earth (6.3kg) and biological flocculant ( 1.26kg), after adsorption, perform plate and frame filtration, and then perform 0.1 μm membrane filtration to obtain mixed solution A;

[0065] (2) adding volume ratio to mixed solution A is that 8:1 mass fraction is 20% ammonium chloride and mass fraction is the ammonium sulfate mixed solution of 35% to obtain solid B after salting out, after solid B is redissolved, The obtained complex solution is first filtered with a 5-micron filter membrane, the obtained filtrate a is then filtered with a 3-micron filter membrane, and the obtained filtrate b is then filtered with a 0.45-micron filter membrane to obtain a mixed solution C;

[0066] (3) The mixture C w...

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Abstract

The invention provides a collagen purification method and relates to the technical field of protein processing. The present invention adopts diatomite + biological flocculant to precipitate components such as insolubles and non-acid-soluble proteins in the solution, and then removes them by plate and frame filtration, which is not only easy to operate, but also can save subsequent ultrafiltration time and improve The yield and the purity of collagen protein; use mass fraction in the salting-out process and be the mixture of the ammonium chloride of 15-25% and the ammonium sulfate of 30-40% in mass fraction, and control the volume ratio of the two to be 5-10: 1. It can significantly increase the amount of collagen precipitation, thereby increasing the yield of collagen; finally, the mixed solution is desalted by using ultrafiltration membrane combined with tubular ultrafiltration membrane, which can remove all the salt in the salting out process , to ensure the purity of the obtained collagen, thereby avoiding the decrease in stability caused by the salt contained in the collagen.

Description

technical field [0001] The invention relates to the technical field of protein processing, in particular to a collagen purification method. Background technique [0002] Collagen, a natural biopolymer produced in animal fibroblasts, accounts for one-third of the total protein in mammals. Structurally, collagen molecules exist in the extracellular matrix in a supramolecular structure. The most common structural feature of collagen is the triple helix structure, which is composed of three α-polypeptide chains, and the polypeptide chains are intertwined in a right-handed helical configuration to form a superhelical structure, which makes the collagen molecule very stable. Due to a series of characteristics in its structure and composition, collagen has good properties such as repair, biocompatibility, mechanical elasticity, and low immune prototype. Collagen and related derivative products are widely used in food, beauty, medicine, biology, etc. It has a wide range of applica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/78C07K1/34C07K1/30C07K1/36
CPCC07K14/78
Inventor 张智武陈新亮彭新生周艳芳
Owner 浙江崇山生物制品有限公司
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