A method for collagen purification

A technology of collagen and purification methods, applied in peptide preparation methods, chemical instruments and methods, animal/human proteins, etc., can solve the problems of unsatisfactory collagen yield and purity, low risk, etc., and achieve improved yield and purity, increase the amount of precipitation, the effect of low cost
CN113105541BActive Publication Date: 2022-03-11浙江崇山生物制品有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
浙江崇山生物制品有限公司
Publication Date
2022-03-11

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Abstract

The invention provides a collagen purification method and relates to the technical field of protein processing. The present invention adopts diatomite + biological flocculant to precipitate components such as insolubles and non-acid-soluble proteins in the solution, and then removes them by plate and frame filtration, which is not only easy to operate, but also can save subsequent ultrafiltration time and improve The yield and the purity of collagen protein; use mass fraction in the salting-out process and be the mixture of the ammonium chloride of 15-25% and the ammonium sulfate of 30-40% in mass fraction, and control the volume ratio of the two to be 5-10: 1. It can significantly increase the amount of collagen precipitation, thereby increasing the yield of collagen; finally, the mixed solution is desalted by using ultrafiltration membrane combined with tubular ultrafiltration membrane, which can remove all the salt in the salting out process , to ensure the purity of the obtained collagen, thereby avoiding the decrease in stability caused by the salt contained in the collagen.
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Description

technical field

[0001] The invention relates to the technical field of protein processing, in particular to a collagen purification method. Background technique

[0002] Collagen, a natural biopolymer produced in animal fibroblasts, accounts for one-third of the total protein in mammals. Structurally, collagen molecules exist in the extracellular matrix in a supramolecular structure. The most common structural feature of collagen is the triple helix structure, which is composed of three α-polypeptide chains, and the polypeptide chains are intertwined in a right-handed helical configuration to form a superhelical structure, which makes the collagen molecule very stable. Due to a series of characteristics in its structure and composition, collagen has good properties such as repair, biocompatibility, mechanical elasticity, and low immune prototype. Collagen and related derivative products are widely used in food, beauty, medicine, biology, etc. It has a wide range of applica...

Claims

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