Application of Ponesimod compound in preparation of anti-EV71 virus drugs

A compound and virus technology, applied in antiviral agents, pharmaceutical formulations, medical preparations containing active ingredients, etc., to achieve great clinical application prospects, inhibit replication and proliferation, and enhance survival rates

Active Publication Date: 2021-07-23
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, ACT-128800 is rarely reported for antiviral therapy

Method used

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  • Application of Ponesimod compound in preparation of anti-EV71 virus drugs
  • Application of Ponesimod compound in preparation of anti-EV71 virus drugs
  • Application of Ponesimod compound in preparation of anti-EV71 virus drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] In this example, the above compounds perform anti-EV71 activity study experiments. Experiments are as follows: The materials and operation methods used in the art and the method of operation are well known in the art.

[0027] 1. Test content:

[0028] Compound anti-EV71 activity analysis: The present invention will bind to cellular lesions effect analysis and MTT assay cell survival detection method, and evaluate ACT-128800 anti-EV71 activity.

[0029] 2. Test method:

[0030] 2.1.1 Toxicity of compounds for host RD cells

[0031] RD cell flooring 96-well plate, at 37 ° C, 5% CO 2 After the incubator is filled with a single layer, the decycle culture liquid is discarded, and the cellular maintenance liquid of different concentrations of test compounds continues to culture, and the microscope test is recorded after 48 hours, and its cytotoxicity is recorded, and the cell survival rate is determined. SPSS11.5 software Computational drugs for half poisoning concentrations of ...

Embodiment 2

[0043] In this embodiment, the ACT-128800 has a deep study of anti-EV71 activity, and the inhibitory effect of the compound is carried out for the Yield of EV71 sub-virus. The test is as follows:

[0044] 1, test content

[0045] After the EV71 infection Rd cells, the compound was suppressed to the yield of EV71 sub-viral production.

[0046] 2, test method

[0047] For several long-term RD cell flooring 24-well plate, 100Tcid after full single layer 50The EV71 infected cells were incubated at 37 ° C for 1.5 h after 1.5 h, and PBS was washed three times, and the cell maintenance liquid containing 50 μm concentration was added.After 48 hours, the cells and supernatants were collected, -20 ° C and 37 ° C, TCID, TCID 50 Method was measured by EV71 viral titer.

[0048] 3, test results

[0049] like image 3 As shown, the compound treated Rd cells relative to the viral control group, and the viral titer has a significant decrease, and the virus is lowered by more than 6.5 Log.

[0050]...

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PUM

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Abstract

The invention discloses an application of a Ponesimod compound in preparation of anti-EV71 (Enterovirus 71) virus drugs. Through anti-EV71 activity research experiments of ACT-128800, the compound ACT-128800 can inhibit cytopathic effects (CPE) generated by EV71 on host cells RD, enhance the cell survival rate and reduce the progeny virus yield, and has the potential to be applied to preparation of anti-EV71 virus drugs.

Description

Technical field [0001] The present invention relates to the field of antiviral drugs, and more particularly to the application of POidence in preparing anti-EV71 viral drugs. Background technique [0002] Intestinal virus 71 (EV71) is a member of the small RNAviridae intestinal virus (ENTEROVIRUS), which is one of the most important pathogens of infant hand and foot disease, sometimes accompanied by severe central nervous system complications. Including aseptic meningitis, encephalitis, spinal cordprisefitis, neuroprosis failure, etc., and even lead to death. Since the first report in 1974, EV71 infectious diseases have repeatedly burst into popularity in the world, especially in the Asia-Pacific region, especially in my country. In view of the great harm of the spread of hand and mouth diseases, my country's government has listed hand and foot and mouth diseases in 2008, and formulated a series of relevant laws and regulations and strict control of hand and foot. Popularization ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/426A61P31/14
CPCA61K31/426A61P31/14Y02A50/30
Inventor 魏艳红胡康洪李涵洛胡达刘会会
Owner HUBEI UNIV OF TECH
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