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Application of Bephenium Hydroxynaphthoate in preparation of anti-EV71 medicine

A technology of drugs and preparations, which is applied in the application field of preparing anti-EV71 drugs to achieve the effects of enhancing survival rate, inhibiting replication and proliferation, and having great clinical application prospects.

Active Publication Date: 2021-08-20
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Bephenium Hydroxynaphthoate is rarely reported for antiviral therapy

Method used

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  • Application of Bephenium Hydroxynaphthoate in preparation of anti-EV71 medicine
  • Application of Bephenium Hydroxynaphthoate in preparation of anti-EV71 medicine
  • Application of Bephenium Hydroxynaphthoate in preparation of anti-EV71 medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] In this embodiment, Bephenium Hydroxynaphthoate is used as an anti-EV71 virus, and it is found that Bephenium Hydroxynaphthoate has an obvious anti-EV71 virus effect. This shows that the compound has the potential to prepare a specific therapeutic drug against EV71 infection, and has great clinical application prospects.

[0025] In this example, the research experiment on the anti-EV71 activity of the above-mentioned compounds is carried out, and the experimental conditions are as follows: In the following, unless otherwise specified, the materials and operation methods used in the present invention are well known in the art.

[0026] 1. Test content:

[0027] Anti-EV71 activity analysis of compounds: In this example, the anti-EV71 activity of Bephenium Hydroxynaphthoate will be evaluated by combining cytopathic effect analysis and MTT assay cell survival rate detection method.

[0028] 2. Test method:

[0029] 2.1.1 Toxicity of compounds to host RD cells

[0030] P...

Embodiment 2

[0043]In this embodiment, Bephenium Hydroxynaphthoate has carried out an in-depth study on the anti-EV71 activity, and implemented the inhibitory effect test of the compound on the production of EV71 progeny virus. The test conditions are as follows:

[0044] 1. Test content

[0045] After EV71 infected RD cells, the inhibitory effect of the compound on the production of EV71 progeny virus was detected.

[0046] 2. Test method

[0047] RD cells in the logarithmic growth phase were plated in 24-well plates, and 100TCID after the monolayer was overgrown 50 EV71 infected cells, incubated at 37°C for 1.5h, removed the virus solution, washed three times with PBS, and added cell maintenance solution containing 3.125μM concentration. After 48 hours, the cells and supernatant were collected and lysed by freezing and thawing three times at -20°C and 37°C. TCID 50 Methods The titer of EV71 virus was determined.

[0048] 3. Test results

[0049] Such as image 3 As shown, compared...

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Abstract

The invention discloses an application of Bephenium Hydroxynaphthoate in the preparation of an anti-EV71 (Enterovirus 71) medicine. Anti-EV71 activity research experiments of the Bephenium Hydroxynaphthoate shows that the compound Bephenium Hydroxynaphthoate inhibits the cytopathic effect (CPE) generated by EV71 on a host cell line RD, the cell survival rate is increased, the progeny virus yield is reduced, and the compound Bephenium Hydroxynaphthoate has the potential to be applied to preparation of the anti-EV71 virus medicine.

Description

technical field [0001] The invention relates to the technical field of antiviral drugs, in particular to the application of Bephenium Hydroxynaphthoate in the preparation of anti-EV71 drugs. Background technique [0002] Enterovirus 71 (EV71) is a member of the genus Enterovirus in the Picornaviridae family, and is one of the most important pathogens causing hand, foot and mouth disease in infants, sometimes accompanied by severe central nervous system complications. Including aseptic meningitis, encephalitis, poliomyelitis-like paralysis, nervous cardiopulmonary failure, etc., and even death. Since the first report in 1974, EV71 infectious disease has broken out and spread many times around the world, especially in the Asia-Pacific region, especially in my country. In view of the great harm that the spread of hand, foot and mouth disease has brought to the lives and health of our people, the Chinese government has listed hand, foot and mouth disease as a Class C infectious...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/205A61P31/14
CPCA61K31/205A61P31/14Y02A50/30
Inventor 魏艳红刘会会胡达胡康洪李涵洛
Owner HUBEI UNIV OF TECH
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