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Application of iodo-hydrazide and iodo-hydrazide polyacid derivative in preparation of anti-CVB3 virus drugs

An iodohydrazide and acid derivative technology, applied in the field of biomedicine, can solve problems such as remote practical application, and achieve the effects of inhibiting cytopathic effect, enhancing survival rate, and promising clinical application.

Active Publication Date: 2022-05-10
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many researchers have found many compounds that can inhibit CVB3 activity in vivo and in vitro, but these are basically still in the initial stage of laboratory experiments, and they are still far away from the actual clinical application

Method used

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  • Application of iodo-hydrazide and iodo-hydrazide polyacid derivative in preparation of anti-CVB3 virus drugs
  • Application of iodo-hydrazide and iodo-hydrazide polyacid derivative in preparation of anti-CVB3 virus drugs
  • Application of iodo-hydrazide and iodo-hydrazide polyacid derivative in preparation of anti-CVB3 virus drugs

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1, Detection of Iodohydrazide and Iodohydrazide Polyacid Derivatives Inhibiting CVB3 Antiviral Activity

[0031] 1. Test content:

[0032] Anti-CVB3 activity analysis of compounds: In this invention, the anti-CVB3 activity of a series of iodohydrazides and iodohydrazide polyacid derivatives with new structures will be evaluated by combining cytopathic effect analysis and MTT determination of cell survival rate detection methods.

[0033] 2. Test method:

[0034] 2.1 Toxicity of compounds to host Hep-2 cells

[0035] Hep-2 cells were plated in 96-well plates at 37°C, 5% CO 2 After the monolayer was grown in the incubator, the cell culture solution was discarded, and the cell maintenance solution containing different concentrations of the test compound was added to continue the culture. After 48 hours, the cytotoxicity was visually observed under the microscope and recorded respectively, and the cell survival rate was determined by the MTT method. SPSS 11.5 sof...

Embodiment 2

[0047] Embodiment 2, the inhibitory action test of compound to CVB3 progeny virus output

[0048] 1. Test content

[0049] After CVB3 infected Hep-2 cells, the inhibitory effect of compound L2 and POM-2 on the production of CVB3 progeny virus was detected.

[0050] 2. Test method

[0051] Hep-2 cells in the logarithmic growth phase were plated on 24-well plates, and the cells were infected with 100TCID50 CVB3 after the monolayer was overgrown. After incubation at 37°C for 1.5h, the virus solution was removed, washed with PBS three times, and the solution containing 25μg / mL L2 and POM-2 was added. Cell maintenance fluid. After 48 hours, the cells and supernatant culture fluid were collected, and after freezing and thawing three times at -20°C and 37°C, the titer of CVB3 virus was determined by the TCID50 method.

[0052] 3. Test results

[0053] Such as image 3 As shown, compared with the virus control group, the Hep-2 cells treated with 25 μg / mL L2 and POM-2 had signific...

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Abstract

The invention provides an application of iodo-hydrazide and an iodo-hydrazide polyacid derivative in preparation of an anti-CVB3 virus drug, and the iodo-hydrazide comprises at least one of p-iodo-hydrazide and 2, 3, 5-iodo-hydrazide; the iodinated hydrazide polyacid derivative comprises POM-0, POM-1 and POM-2, and the structural formula of the iodinated hydrazide polyacid derivative is shown in the specification; the application finds that the iodo-hydrazide and the iodo-hydrazide polyacid derivative have the activity of resisting the CVB3 virus. The cytopathic effect caused by the CVB3 virus can be inhibited, the survival rate of infected cells can be enhanced, the replication and proliferation of the CVB3 virus in the cells can be inhibited, and the yield of progeny viruses can be reduced. Therefore, the compound has the potential to prepare specific treatment medicines for resisting CVB3 infection, and has a great clinical application prospect.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of iodohydrazide and iodohydrazide polyacid derivatives in the preparation of anti-CVB3 virus drugs. Background technique [0002] Coxsackievirus (Coxsaekievirus) is a member of the genus Enterovirus of the family Picornaviridae, and its infection can cause a variety of diseases, such as hand, foot and mouth disease, aseptic meningitis, encephalitis, myocarditis, epidemic Myositis pain, herpetic angina, etc. There are 29 serotypes of CV reported, which can be divided into two groups, A and B, according to their pathogenic characteristics and cell sensitivity to suckling mice, namely CVA (CVA1-22, 24) and CVB ( CVB1-6). CVBs infection is the most common, among which CVB3 is the most pathogenic type among the six CVB serotypes, and is the main cause of viral myocarditis. At present, there is no specific drug for Coxsackie virus infection, and there is no targ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/166A61K33/24A61P31/14
CPCA61K31/166A61K33/24A61P31/14Y02A50/30
Inventor 魏艳红王龙胜胡达胡康洪李涵泺郭超董宇嘉
Owner HUBEI UNIV OF TECH
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